(Linear) (Six-base) MAPSORT of: KIBB6733.seq Check: 6371 from: 1 to: 285 KIBB6733.orf With 182 enzymes: SgfI * April 24, 2009 13:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 225 285 Size: 225 60 AccI GT'mk_AC Cuts at: 0 281 285 Size: 281 4 AseI AT'TA_AT Cuts at: 0 53 285 Size: 53 232 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 203 285 Size: 109 94 82 Fragments arranged by size: 109 94 82 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 225 285 Size: 225 60 EaeI y'GGCC_r Cuts at: 0 2 285 Size: 2 283 MfeI C'AATT_G Cuts at: 0 248 285 Size: 248 37 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 206 285 Size: 206 79 StyI C'CwwG_G Cuts at: 0 17 285 Size: 17 268 Enzymes that do cut: AarI AccI AseI BsgI BspMI EaeI MfeI MmeI StyI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI