(Linear) (Six-base) MAPSORT of: KIBB6731.seq  Check: 9485  from: 1  to: 285



KIBB6731.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:      0     230     285 
   Size:        230      55
 
AflIII A'CryG_T

Cuts at:      0     158     285 
   Size:        158     127
 
AlwNI CAG_nnn'CTG

Cuts at:      0     142     285 
   Size:        142     143
 
BanII G_rGCy'C

Cuts at:      0     193     285 
   Size:        193      92
 
Bpu10I CC'TnA_GC

Cuts at:      0      67     285 
   Size:         67     218
 
BsaAI yAC'GTr

Cuts at:      0     207     285 
   Size:        207      78
 
BsaBI GATnn'nnATC

Cuts at:      0     101     285 
   Size:        101     184
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     285 
   Size:        229      56
 
Bsp1286I G_dGCh'C

Cuts at:      0     193     285 
   Size:        193      92
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     130     285 
   Size:        130     155
 
EarI CTCTTCn'nnn_

Cuts at:      0      10     285 
   Size:         10     275
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     195     285 
   Size:        195      90
 
HincII GTy'rAC

Cuts at:      0     231     285 
   Size:        231      54
 
MspA1I CmG'CkG

Cuts at:      0     213     285 
   Size:        213      72
 
NspI r_CATG'y

Cuts at:      0     162     285 
   Size:        162     123
 
PciI A'CATG_T

Cuts at:      0     158     285 
   Size:        158     127
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      93     285 
   Size:         93     192
 
PstI C_TGCA'G

Cuts at:      0     275     285 
   Size:        275      10
 
PvuII CAG'CTG

Cuts at:      0     213     285 
   Size:        213      72
 
SalI G'TCGA_C

Cuts at:      0     229     285 
   Size:        229      56
 
SapI GCTCTTCn'nnn_

Cuts at:      0      10     285 
   Size:         10     275
 
SfcI C'TryA_G

Cuts at:      0     244     271     285 
   Size:        244      27      14

  Fragments arranged by size: 

                 244      27      14
 
XmnI GAAnn'nnTTC

Cuts at:      0     178     285 
   Size:        178     107
 

 Enzymes that do cut:

     AccI   AflIII    AlwNI    BanII   Bpu10I    BsaAI    BsaBI     BsgI
 Bsp1286I     DrdI     EarI     FalI   HincII   MspA1I     NspI     PciI
    PflMI     PstI    PvuII     SalI     SapI     SfcI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI