(Linear) (Six-base) MAPSORT of: KIBB6727.seq  Check: 3742  from: 1  to: 276



KIBB6727.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AhdI GACnn_n'nnGTC

Cuts at:      0      99     276 
   Size:         99     177
 
AlwNI CAG_nnn'CTG

Cuts at:      0     105     276 
   Size:        105     171
 
BamHI G'GATC_C

Cuts at:      0     134     276 
   Size:        134     142
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     141     175     276 
   Size:        141      34     101

  Fragments arranged by size: 

                 141     101      34
 
BlpI GC'TnA_GC

Cuts at:      0     166     276 
   Size:        166     110
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     218     276 
   Size:        218      58
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      43     276 
   Size:         43     233
 
BsaHI Gr'CG_yC

Cuts at:      0       4     276 
   Size:          4     272
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     107     137     276 
   Size:        107      30     139

  Fragments arranged by size: 

                 139     107      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31     241     276 
   Size:         31     210      35

  Fragments arranged by size: 

                 210      35      31
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      11     276 
   Size:         11     265
 
BsrFI r'CCGG_y

Cuts at:      0     179     276 
   Size:        179      97
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     176     276 
   Size:        176     100
 
BstYI r'GATC_y

Cuts at:      0     134     276 
   Size:        134     142
 
BtgI C'CryG_G

Cuts at:      0      47     276 
   Size:         47     229
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     111     276 
   Size:        111     165
 
EaeI y'GGCC_r

Cuts at:      0      12      50     276 
   Size:         12      38     226

  Fragments arranged by size: 

                 226      38      12
 
EarI CTCTTCn'nnn_

Cuts at:      0     145     218     276 
   Size:        145      73      58

  Fragments arranged by size: 

                 145      73      58
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     241     276 
   Size:        241      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      85     107     117     139     276 
   Size:         85      22      10      22     137

  Fragments arranged by size: 

                 137      85      22      22      10
 
MscI TGG'CCA

Cuts at:      0      14      52     276 
   Size:         14      38     224

  Fragments arranged by size: 

                 224      38      14
 
NcoI C'CATG_G

Cuts at:      0      47     276 
   Size:         47     229
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      21     276 
   Size:         21     255
 
PstI C_TGCA'G

Cuts at:      0     110     276 
   Size:        110     166
 
SfcI C'TryA_G

Cuts at:      0     106     276 
   Size:        106     170
 
StuI AGG'CCT

Cuts at:      0     119     276 
   Size:        119     157
 
StyI C'CwwG_G

Cuts at:      0      47      53     276 
   Size:         47       6     223

  Fragments arranged by size: 

                 223      47       6
 

 Enzymes that do cut:

     AhdI    AlwNI    BamHI     BcgI     BlpI     BmrI     BsaI    BsaHI
    BsaXI    BseRI    BsmBI    BsrFI   BstAPI    BstYI     BtgI    BtgZI
     EaeI     EarI     EciI    Hin4I     MscI     NcoI    PflMI     PstI
     SfcI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI