(Linear) (Six-base) MAPSORT of: KIBB6723.seq  Check: 7095  from: 1  to: 273



KIBB6723.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

Bme1580I G_kGCm'C

Cuts at:      0     135     273 
   Size:        135     138
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12     273 
   Size:         12     261
 
BseYI C'CCAG_C

Cuts at:      0     177     273 
   Size:        177      96
 
BsiHKAI G_wGCw'C

Cuts at:      0     172     273 
   Size:        172     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     135     172     273 
   Size:        135      37     101

  Fragments arranged by size: 

                 135     101      37
 
BsrDI GCAATG_nn'

Cuts at:      0      35     110     273 
   Size:         35      75     163

  Fragments arranged by size: 

                 163      75      35
 
BtgI C'CryG_G

Cuts at:      0      11     273 
   Size:         11     262
 
BtsI GCAGTG_nn'

Cuts at:      0     121     273 
   Size:        121     152
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     197     273 
   Size:        197      76
 
MspA1I CmG'CkG

Cuts at:      0      13     273 
   Size:         13     260
 
SacII CC_GC'GG

Cuts at:      0      14     273 
   Size:         14     259
 
TatI w'GTAC_w

Cuts at:      0     248     273 
   Size:        248      25
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     233     273 
   Size:        233      40
 

 Enzymes that do cut:

 Bme1580I     BsaI    BseYI  BsiHKAI Bsp1286I    BsrDI     BtgI     BtsI
     MmeI   MspA1I    SacII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI