(Linear) (Six-base) MAPSORT of: KIBB6722.seq  Check: 6309  from: 1  to: 402



KIBB6722.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AfeI AGC'GCT

Cuts at:      0     205     402 
   Size:        205     197
 
AlwNI CAG_nnn'CTG

Cuts at:      0      12     255     267     402 
   Size:         12     243      12     135

  Fragments arranged by size: 

                 243     135      12      12
 
BanII G_rGCy'C

Cuts at:      0      84     242     355     402 
   Size:         84     158     113      47

  Fragments arranged by size: 

                 158     113      84      47
 
Bpu10I CC'TnA_GC

Cuts at:      0      83     162     402 
   Size:         83      79     240

  Fragments arranged by size: 

                 240      83      79
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     402 
   Size:         82     320
 
BsaWI w'CCGG_w

Cuts at:      0     143     402 
   Size:        143     259
 
BseYI C'CCAG_C

Cuts at:      0     177     402 
   Size:        177     225
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     268     402 
   Size:        245      23     134

  Fragments arranged by size: 

                 245     134      23
 
BsiHKAI G_wGCw'C

Cuts at:      0     242     402 
   Size:        242     160
 
Bsp1286I G_dGCh'C

Cuts at:      0      84     242     355     402 
   Size:         84     158     113      47

  Fragments arranged by size: 

                 158     113      84      47
 
BsrBI CCG'CTC

Cuts at:      0     378     402 
   Size:        378      24
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     258     402 
   Size:        258     144
 
BtsI GCAGTG_nn'

Cuts at:      0      75     402 
   Size:         75     327
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     378     402 
   Size:        378      24
 
EcoICRI GAG'CTC

Cuts at:      0     240     402 
   Size:        240     162
 
EcoO109I rG'GnC_Cy

Cuts at:      0      59     323     402 
   Size:         59     264      79

  Fragments arranged by size: 

                 264      79      59
 
HaeII r_GCGC'y

Cuts at:      0     207     402 
   Size:        207     195
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     249     281     402 
   Size:        249      32     121

  Fragments arranged by size: 

                 249     121      32
 
HindIII A'AGCT_T

Cuts at:      0     159     402 
   Size:        159     243
 
MspA1I CmG'CkG

Cuts at:      0     255     402 
   Size:        255     147
 
PasI CC'CwG_GG

Cuts at:      0     300     402 
   Size:        300     102
 
PpuMI rG'GwC_Cy

Cuts at:      0      59     402 
   Size:         59     343
 
PstI C_TGCA'G

Cuts at:      0     310     402 
   Size:        310      92
 
PvuII CAG'CTG

Cuts at:      0     255     402 
   Size:        255     147
 
SacI G_AGCT'C

Cuts at:      0     242     402 
   Size:        242     160
 
SfcI C'TryA_G

Cuts at:      0     306     402 
   Size:        306      96
 
SmlI C'TyrA_G

Cuts at:      0      97     402 
   Size:         97     305
 
StyI C'CwwG_G

Cuts at:      0      63     125     402 
   Size:         63      62     277

  Fragments arranged by size: 

                 277      63      62
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     234     402 
   Size:        234     168
 
TatI w'GTAC_w

Cuts at:      0     101     402 
   Size:        101     301
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      70     239     402 
   Size:         70     169     163

  Fragments arranged by size: 

                 169     163      70
 

 Enzymes that do cut:

     AfeI    AlwNI    BanII   Bpu10I    BpuEI    BsaWI    BseYI     BsgI
  BsiHKAI Bsp1286I    BsrBI   BstAPI     BtsI     DrdI  EcoICRI EcoO109I
    HaeII    Hin4I  HindIII   MspA1I     PasI    PpuMI     PstI    PvuII
     SacI     SfcI     SmlI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI