(Linear) (Six-base) MAPSORT of: KIBB6718.seq  Check: 9687  from: 1  to: 402



KIBB6718.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     402 
   Size:        212     190
 
AflIII A'CryG_T

Cuts at:      0      47     402 
   Size:         47     355
 
ApaLI G'TGCA_C

Cuts at:      0     186     402 
   Size:        186     216
 
ApoI r'AATT_y

Cuts at:      0      68     402 
   Size:         68     334
 
Bme1580I G_kGCm'C

Cuts at:      0     190     402 
   Size:        190     212
 
BmtI G_CTAG'C

Cuts at:      0      31     402 
   Size:         31     371
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     402 
   Size:        241     161
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      88     402 
   Size:         88     314
 
BsaAI yAC'GTr

Cuts at:      0      48     402 
   Size:         48     354
 
BsiHKAI G_wGCw'C

Cuts at:      0     190     402 
   Size:        190     212
 
Bsp1286I G_dGCh'C

Cuts at:      0     190     402 
   Size:        190     212
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     362     402 
   Size:        362      40
 
BsrGI T'GTAC_A

Cuts at:      0     165     402 
   Size:        165     237
 
BtsI GCAGTG_nn'

Cuts at:      0     103     402 
   Size:        103     299
 
DraIII CAC_nnn'GTG

Cuts at:      0     386     402 
   Size:        386      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     241     402 
   Size:        212      29     161

  Fragments arranged by size: 

                 212     161      29
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     382     402 
   Size:        382      20
 
NheI G'CTAG_C

Cuts at:      0      27     402 
   Size:         27     375
 
StyI C'CwwG_G

Cuts at:      0      98     402 
   Size:         98     304
 
TatI w'GTAC_w

Cuts at:      0     165     402 
   Size:        165     237
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      54      86     402 
   Size:         54      32     316

  Fragments arranged by size: 

                 316      54      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      81     402 
   Size:         81     321
 
XmnI GAAnn'nnTTC

Cuts at:      0     200     402 
   Size:        200     202
 

 Enzymes that do cut:

     AcuI   AflIII    ApaLI     ApoI Bme1580I     BmtI     BpmI     BsaI
    BsaAI  BsiHKAI Bsp1286I    BspMI    BsrGI     BtsI   DraIII  Eco57MI
     FalI     NheI     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BplI   Bpu10I    BpuEI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     ZraI