Restriction map for
pF1KB6698
(Linear) (Six-base) MAPSORT of: KIBB6698.seq Check: 4524 from: 1 to: 1335
KIBB6698.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 1335
Size: 721 614
AccI GT'mk_AC
Cuts at: 0 338 1335
Size: 338 997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 483 664 1102 1335
Size: 237 246 181 438 233
Fragments arranged by size:
438 246 237 233 181
AflIII A'CryG_T
Cuts at: 0 143 1335
Size: 143 1192
AhdI GACnn_n'nnGTC
Cuts at: 0 1254 1335
Size: 1254 81
AleI CACnn'nnGTG
Cuts at: 0 535 1335
Size: 535 800
AlwNI CAG_nnn'CTG
Cuts at: 0 1128 1335
Size: 1128 207
ApaLI G'TGCA_C
Cuts at: 0 13 1335
Size: 13 1322
AvaI C'yCGr_G
Cuts at: 0 182 726 859 1335
Size: 182 544 133 476
Fragments arranged by size:
544 476 182 133
BamHI G'GATC_C
Cuts at: 0 1031 1335
Size: 1031 304
BanI G'GyrC_C
Cuts at: 0 49 211 442 771 855 1335
Size: 49 162 231 329 84 480
Fragments arranged by size:
480 329 231 162 84 49
BanII G_rGCy'C
Cuts at: 0 545 866 1335
Size: 545 321 469
Fragments arranged by size:
545 469 321
BbeI G_GCGC'C
Cuts at: 0 53 1335
Size: 53 1282
BbsI GAAGACnn'nnnn_
Cuts at: 0 1054 1335
Size: 1054 281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 61 1002 1036 1335
Size: 27 34 941 34 299
Fragments arranged by size:
941 299 34 34 27
BglI GCCn_nnn'nGGC
Cuts at: 0 35 1335
Size: 35 1300
BglII A'GATC_T
Cuts at: 0 248 1335
Size: 248 1087
Bme1580I G_kGCm'C
Cuts at: 0 17 445 776 860 1335
Size: 17 428 331 84 475
Fragments arranged by size:
475 428 331 84 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 1335
Size: 309 1026
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 223 373 1335
Size: 151 72 150 962
Fragments arranged by size:
962 151 150 72
BsaHI Gr'CG_yC
Cuts at: 0 50 1280 1335
Size: 50 1230 55
Fragments arranged by size:
1230 55 50
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1324 1327 1330 1335
Size: 1324 3 3 5
Fragments arranged by size:
1324 5 3 3
BseYI C'CCAG_C
Cuts at: 0 869 1335
Size: 869 466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1018 1168 1335
Size: 772 246 150 167
Fragments arranged by size:
772 246 167 150
BsiEI CG_ry'CG
Cuts at: 0 924 1055 1289 1335
Size: 924 131 234 46
Fragments arranged by size:
924 234 131 46
BsiHKAI G_wGCw'C
Cuts at: 0 17 866 1335
Size: 17 849 469
Fragments arranged by size:
849 469 17
Bsp1286I G_dGCh'C
Cuts at: 0 17 445 545 776 860 866 1335
Size: 17 428 100 231 84 6 469
Fragments arranged by size:
469 428 231 100 84 17 6
BspHI T'CATG_A
Cuts at: 0 488 1335
Size: 488 847
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 721 755 1335
Size: 721 34 580
Fragments arranged by size:
721 580 34
BsrBI CCG'CTC
Cuts at: 0 701 1335
Size: 701 634
BstEII G'GTnAC_C
Cuts at: 0 705 1335
Size: 705 630
BstXI CCAn_nnnn'nTGG
Cuts at: 0 195 1335
Size: 195 1140
BstYI r'GATC_y
Cuts at: 0 198 248 461 1031 1335
Size: 198 50 213 570 304
Fragments arranged by size:
570 304 213 198 50
BtgI C'CryG_G
Cuts at: 0 108 215 918 1074 1283 1335
Size: 108 107 703 156 209 52
Fragments arranged by size:
703 209 156 108 107 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 89 1335
Size: 89 1246
DraIII CAC_nnn'GTG
Cuts at: 0 684 1335
Size: 684 651
EaeI y'GGCC_r
Cuts at: 0 243 276 689 899 921 1052 1091 1115
Size: 243 33 413 210 22 131 39 24
Cuts at: 1115 1157 1286 1335
Size: 42 129 49
Fragments arranged by size:
413 243 210 131 129 49 42 39
33 24 22
EagI C'GGCC_G
Cuts at: 0 921 1052 1286 1335
Size: 921 131 234 49
Fragments arranged by size:
921 234 131 49
EarI CTCTTCn'nnn_
Cuts at: 0 464 1335
Size: 464 871
EcoICRI GAG'CTC
Cuts at: 0 864 1335
Size: 864 471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 223 237 373 483 664 1102 1335
Size: 151 72 14 136 110 181 438 233
Fragments arranged by size:
438 233 181 151 136 110 72 14
EcoO109I rG'GnC_Cy
Cuts at: 0 615 669 1335
Size: 615 54 666
Fragments arranged by size:
666 615 54
FspI TGC'GCA
Cuts at: 0 751 1335
Size: 751 584
HaeII r_GCGC'y
Cuts at: 0 53 850 1335
Size: 53 797 485
Fragments arranged by size:
797 485 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 396 428 1240 1272 1335
Size: 396 32 812 32 63
Fragments arranged by size:
812 396 63 32 32
HincII GTy'rAC
Cuts at: 0 339 1335
Size: 339 996
KasI G'GCGC_C
Cuts at: 0 49 1335
Size: 49 1286
MslI CAynn'nnrTG
Cuts at: 0 193 535 964 1159 1335
Size: 193 342 429 195 176
Fragments arranged by size:
429 342 195 193 176
MspA1I CmG'CkG
Cuts at: 0 129 402 573 735 1076 1335
Size: 129 273 171 162 341 259
Fragments arranged by size:
341 273 259 171 162 129
NarI GG'CG_CC
Cuts at: 0 50 1335
Size: 50 1285
NcoI C'CATG_G
Cuts at: 0 108 215 1335
Size: 108 107 1120
Fragments arranged by size:
1120 108 107
NspI r_CATG'y
Cuts at: 0 963 1335
Size: 963 372
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 414 573 576 1335
Size: 59 355 159 3 759
Fragments arranged by size:
759 355 159 59 3
PfoI T'CCnGG_A
Cuts at: 0 347 433 464 1121 1335
Size: 347 86 31 657 214
Fragments arranged by size:
657 347 214 86 31
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1204 1236 1335
Size: 1204 32 99
Fragments arranged by size:
1204 99 32
PpuMI rG'GwC_Cy
Cuts at: 0 669 1335
Size: 669 666
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1335
Size: 1000 32 303
Fragments arranged by size:
1000 303 32
PvuII CAG'CTG
Cuts at: 0 129 402 573 735 1335
Size: 129 273 171 162 600
Fragments arranged by size:
600 273 171 162 129
SacI G_AGCT'C
Cuts at: 0 866 1335
Size: 866 469
SacII CC_GC'GG
Cuts at: 0 1077 1335
Size: 1077 258
SalI G'TCGA_C
Cuts at: 0 337 1335
Size: 337 998
SexAI A'CCwGG_T
Cuts at: 0 680 1250 1335
Size: 680 570 85
Fragments arranged by size:
680 570 85
SfoI GGC'GCC
Cuts at: 0 51 1335
Size: 51 1284
SmaI CCC'GGG
Cuts at: 0 728 1335
Size: 728 607
StuI AGG'CCT
Cuts at: 0 1177 1335
Size: 1177 158
StyI C'CwwG_G
Cuts at: 0 108 215 305 518 1335
Size: 108 107 90 213 817
Fragments arranged by size:
817 213 108 107 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 333 531 552 883 1335
Size: 333 198 21 331 452
Fragments arranged by size:
452 333 331 198 21
TatI w'GTAC_w
Cuts at: 0 146 1335
Size: 146 1189
Tth111I GACn'n_nGTC
Cuts at: 0 223 343 1335
Size: 223 120 992
Fragments arranged by size:
992 223 120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 894 972 1335
Size: 894 78 363
Fragments arranged by size:
894 363 78
XmaI C'CCGG_G
Cuts at: 0 726 1335
Size: 726 609
XmnI GAAnn'nnTTC
Cuts at: 0 496 1335
Size: 496 839
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlwNI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BcgI BglI
BglII Bme1580I BmrI BpmI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BstEII BstXI
BstYI BtgI BtgZI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoO109I FspI HaeII Hin4I HincII KasI MslI
MspA1I NarI NcoI NspI PflMI PfoI PpiI PpuMI
PsrI PvuII SacI SacII SalI SexAI SfoI SmaI
StuI StyI TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AlfI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BciVI BclI BfrBI BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI
BsmBI BspEI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EciI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII
HpaI KpnI MfeI MluI MmeI MscI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PshAI PsiI PspOMI PspXI PstI PvuI
RsrII SanDI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TstI XbaI XhoI ZraI