Restriction map for
pF1KB6695
(Linear) (Six-base) MAPSORT of: KIBB6695.seq Check: 5592 from: 1 to: 1287
KIBB6695.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1107 1287
Size: 1107 180
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1010 1287
Size: 361 649 277
Fragments arranged by size:
649 361 277
AhdI GACnn_n'nnGTC
Cuts at: 0 126 642 1287
Size: 126 516 645
Fragments arranged by size:
645 516 126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 458 832 1287
Size: 458 374 455
Fragments arranged by size:
458 455 374
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 1287
Size: 27 1260
AlwNI CAG_nnn'CTG
Cuts at: 0 177 1287
Size: 177 1110
ApaI G_GGCC'C
Cuts at: 0 277 1170 1287
Size: 277 893 117
Fragments arranged by size:
893 277 117
ApaLI G'TGCA_C
Cuts at: 0 410 1287
Size: 410 877
BamHI G'GATC_C
Cuts at: 0 1227 1287
Size: 1227 60
BanI G'GyrC_C
Cuts at: 0 1119 1287
Size: 1119 168
BanII G_rGCy'C
Cuts at: 0 144 277 589 1064 1170 1287
Size: 144 133 312 475 106 117
Fragments arranged by size:
475 312 144 133 117 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 398 1287
Size: 398 889
BbvCI CC'TCA_GC
Cuts at: 0 428 1287
Size: 428 859
BclI T'GATC_A
Cuts at: 0 725 1287
Size: 725 562
BfrBI ATG'CAT
Cuts at: 0 809 1287
Size: 809 478
BglI GCCn_nnn'nGGC
Cuts at: 0 1068 1287
Size: 1068 219
Bme1580I G_kGCm'C
Cuts at: 0 277 414 475 1124 1170 1287
Size: 277 137 61 649 46 117
Fragments arranged by size:
649 277 137 117 61 46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 555 1287
Size: 555 732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 235 272 1287
Size: 76 159 37 1015
Fragments arranged by size:
1015 159 76 37
Bpu10I CC'TnA_GC
Cuts at: 0 428 1012 1287
Size: 428 584 275
Fragments arranged by size:
584 428 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1219 1287
Size: 358 861 68
Fragments arranged by size:
861 358 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 1171 1287
Size: 1171 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 248 278 404 434 1268 1287
Size: 25 223 30 126 30 834 19
Fragments arranged by size:
834 223 126 30 30 25 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 269 444 445 496 1285 1287
Size: 269 175 1 51 789 2
Fragments arranged by size:
789 269 175 51 2 1
BseYI C'CCAG_C
Cuts at: 0 744 1287
Size: 744 543
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 1111 1287
Size: 54 1057 176
Fragments arranged by size:
1057 176 54
BsiHKAI G_wGCw'C
Cuts at: 0 103 414 1046 1287
Size: 103 311 632 241
Fragments arranged by size:
632 311 241 103
Bsp1286I G_dGCh'C
Cuts at: 0 103 144 277 414 475 589 1046 1064
Size: 103 41 133 137 61 114 457 18
Cuts at: 1064 1124 1170 1287
Size: 60 46 117
Fragments arranged by size:
457 137 133 117 114 103 61 60
46 41 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1107 1287
Size: 1107 180
BsrFI r'CCGG_y
Cuts at: 0 1112 1287
Size: 1112 175
BstAPI GCAn_nnn'nTGC
Cuts at: 0 924 1287
Size: 924 363
BstXI CCAn_nnnn'nTGG
Cuts at: 0 552 1287
Size: 552 735
BstYI r'GATC_y
Cuts at: 0 174 1227 1287
Size: 174 1053 60
Fragments arranged by size:
1053 174 60
Bsu36I CC'TnA_GG
Cuts at: 0 365 524 1287
Size: 365 159 763
Fragments arranged by size:
763 365 159
BtgI C'CryG_G
Cuts at: 0 551 1287
Size: 551 736
BtsI GCAGTG_nn'
Cuts at: 0 354 545 1287
Size: 354 191 742
Fragments arranged by size:
742 354 191
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 145 180 250 285 1287
Size: 145 35 70 35 1002
Fragments arranged by size:
1002 145 70 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 58 524 986 1287
Size: 58 466 462 301
Fragments arranged by size:
466 462 301 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 235 272 361 1010 1287
Size: 76 159 37 89 649 277
Fragments arranged by size:
649 277 159 89 76 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 368 1287
Size: 368 919
EcoO109I rG'GnC_Cy
Cuts at: 0 273 274 1166 1287
Size: 273 1 892 121
Fragments arranged by size:
892 273 121 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 571 603 628 660 1268 1287
Size: 112 32 427 32 25 32 608 19
Fragments arranged by size:
608 427 112 32 32 32 25 19
HincII GTy'rAC
Cuts at: 0 131 1287
Size: 131 1156
MslI CAynn'nnrTG
Cuts at: 0 550 832 863 1287
Size: 550 282 31 424
Fragments arranged by size:
550 424 282 31
NcoI C'CATG_G
Cuts at: 0 551 1287
Size: 551 736
NdeI CA'TA_TG
Cuts at: 0 147 1287
Size: 147 1140
NsiI A_TGCA'T
Cuts at: 0 811 1287
Size: 811 476
NspI r_CATG'y
Cuts at: 0 813 927 1142 1287
Size: 813 114 215 145
Fragments arranged by size:
813 215 145 114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1287
Size: 27 1260
PshAI GACnn'nnGTC
Cuts at: 0 224 1287
Size: 224 1063
PspOMI G'GGCC_C
Cuts at: 0 273 1166 1287
Size: 273 893 121
Fragments arranged by size:
893 273 121
SexAI A'CCwGG_T
Cuts at: 0 1103 1287
Size: 1103 184
SfcI C'TryA_G
Cuts at: 0 160 1287
Size: 160 1127
SmlI C'TyrA_G
Cuts at: 0 337 1198 1287
Size: 337 861 89
Fragments arranged by size:
861 337 89
SphI G_CATG'C
Cuts at: 0 813 927 1142 1287
Size: 813 114 215 145
Fragments arranged by size:
813 215 145 114
StuI AGG'CCT
Cuts at: 0 374 1287
Size: 374 913
StyI C'CwwG_G
Cuts at: 0 551 1162 1287
Size: 551 611 125
Fragments arranged by size:
611 551 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 298 397 946 1077 1287
Size: 298 99 549 131 210
Fragments arranged by size:
549 298 210 131 99
TatI w'GTAC_w
Cuts at: 0 1202 1287
Size: 1202 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 248 280 404 436 1287
Size: 80 32 136 32 124 32 851
Fragments arranged by size:
851 136 124 80 32 32 32
XbaI T'CTAG_A
Cuts at: 0 477 1287
Size: 477 810
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 161 247 1287
Size: 161 86 1040
Fragments arranged by size:
1040 161 86
XmnI GAAnn'nnTTC
Cuts at: 0 374 1287
Size: 374 913
Enzymes that do cut:
AarI AcuI AhdI AlfI AloI AlwNI ApaI ApaLI
BamHI BanI BanII BbsI BbvCI BclI BfrBI BglI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstXI
BstYI Bsu36I BtgI BtsI CspCI EarI Eco57MI EcoNI
EcoO109I Hin4I HincII MslI NcoI NdeI NsiI NspI
PpiI PshAI PspOMI SexAI SfcI SmlI SphI StuI
StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BciVI BglII BlpI BmgBI BmrI BmtI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MspA1I NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpuMI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XhoI XmaI ZraI