Restriction map for
pF1KB6694
(Linear) (Six-base) MAPSORT of: KIBB6694.seq Check: 2454 from: 1 to: 1386
KIBB6694.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 0 758 1386
Size: 758 628
AclI AA'CG_TT
Cuts at: 0 851 971 1386
Size: 851 120 415
Fragments arranged by size:
851 415 120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 1386
Size: 1144 242
AflIII A'CryG_T
Cuts at: 0 42 1386
Size: 42 1344
ApoI r'AATT_y
Cuts at: 0 121 235 364 1174 1386
Size: 121 114 129 810 212
Fragments arranged by size:
810 212 129 121 114
AvaI C'yCGr_G
Cuts at: 0 398 1386
Size: 398 988
BanII G_rGCy'C
Cuts at: 0 157 1386
Size: 157 1229
BglI GCCn_nnn'nGGC
Cuts at: 0 415 1386
Size: 415 971
BlpI GC'TnA_GC
Cuts at: 0 194 1118 1386
Size: 194 924 268
Fragments arranged by size:
924 268 194
BmrI ACTGGGnnnn_n'
Cuts at: 0 436 1386
Size: 436 950
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 920 1260 1386
Size: 920 340 126
Fragments arranged by size:
920 340 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 733 827 1358 1386
Size: 625 108 94 531 28
Fragments arranged by size:
625 531 108 94 28
Bpu10I CC'TnA_GC
Cuts at: 0 1025 1386
Size: 1025 361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1386
Size: 706 680
BsaI GGTCTCn'nnnn_
Cuts at: 0 235 1386
Size: 235 1151
BsaAI yAC'GTr
Cuts at: 0 45 1386
Size: 45 1341
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 825 855 1386
Size: 825 30 531
Fragments arranged by size:
825 531 30
Bsp1286I G_dGCh'C
Cuts at: 0 157 1386
Size: 157 1229
BsrDI GCAATG_nn'
Cuts at: 0 669 1100 1386
Size: 669 431 286
Fragments arranged by size:
669 431 286
BsrFI r'CCGG_y
Cuts at: 0 1063 1386
Size: 1063 323
BstEII G'GTnAC_C
Cuts at: 0 831 1350 1386
Size: 831 519 36
Fragments arranged by size:
831 519 36
BtsI GCAGTG_nn'
Cuts at: 0 1019 1325 1386
Size: 1019 306 61
Fragments arranged by size:
1019 306 61
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 651 666 686 701 750 785 1386
Size: 651 15 20 15 49 35 601
Fragments arranged by size:
651 601 49 35 20 15 15
DraIII CAC_nnn'GTG
Cuts at: 0 430 1386
Size: 430 956
DrdI GACnn_nn'nnGTC
Cuts at: 0 334 1386
Size: 334 1052
EaeI y'GGCC_r
Cuts at: 0 72 792 1386
Size: 72 720 594
Fragments arranged by size:
720 594 72
EarI CTCTTCn'nnn_
Cuts at: 0 487 911 1386
Size: 487 424 475
Fragments arranged by size:
487 475 424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 733 827 1144 1358 1386
Size: 625 108 94 317 214 28
Fragments arranged by size:
625 317 214 108 94 28
EcoRV GAT'ATC
Cuts at: 0 222 1386
Size: 222 1164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 174 1386
Size: 174 1212
HaeII r_GCGC'y
Cuts at: 0 1064 1386
Size: 1064 322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 278 310 629 661 1386
Size: 278 32 319 32 725
Fragments arranged by size:
725 319 278 32 32
HincII GTy'rAC
Cuts at: 0 456 849 1386
Size: 456 393 537
Fragments arranged by size:
537 456 393
HindIII A'AGCT_T
Cuts at: 0 890 1386
Size: 890 496
HpaI GTT'AAC
Cuts at: 0 456 1386
Size: 456 930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 745 1386
Size: 565 180 641
Fragments arranged by size:
641 565 180
MscI TGG'CCA
Cuts at: 0 74 1386
Size: 74 1312
MspA1I CmG'CkG
Cuts at: 0 1012 1386
Size: 1012 374
NaeI GCC'GGC
Cuts at: 0 1065 1386
Size: 1065 321
NgoMIV G'CCGG_C
Cuts at: 0 1063 1386
Size: 1063 323
NspI r_CATG'y
Cuts at: 0 409 603 624 1109 1386
Size: 409 194 21 485 277
Fragments arranged by size:
485 409 277 194 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 580 1386
Size: 580 806
PfoI T'CCnGG_A
Cuts at: 0 749 1386
Size: 749 637
PvuII CAG'CTG
Cuts at: 0 1012 1386
Size: 1012 374
SapI GCTCTTCn'nnn_
Cuts at: 0 911 1386
Size: 911 475
ScaI AGT'ACT
Cuts at: 0 253 1386
Size: 253 1133
SfcI C'TryA_G
Cuts at: 0 483 1386
Size: 483 903
SmlI C'TyrA_G
Cuts at: 0 685 1386
Size: 685 701
SphI G_CATG'C
Cuts at: 0 409 1109 1386
Size: 409 700 277
Fragments arranged by size:
700 409 277
StyI C'CwwG_G
Cuts at: 0 625 1386
Size: 625 761
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 418 1294 1386
Size: 418 876 92
Fragments arranged by size:
876 418 92
TatI w'GTAC_w
Cuts at: 0 251 1386
Size: 251 1135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 825 857 1386
Size: 825 32 529
Fragments arranged by size:
825 529 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 581 1206 1386
Size: 581 625 180
Fragments arranged by size:
625 581 180
XmnI GAAnn'nnTTC
Cuts at: 0 905 1386
Size: 905 481
Enzymes that do cut:
AccI AclI AcuI AflIII ApoI AvaI BanII BglI
BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaXI Bsp1286I BsrDI BsrFI BstEII BtsI CspCI DraIII
DrdI EaeI EarI Eco57MI EcoRV FalI HaeII Hin4I
HincII HindIII HpaI MmeI MscI MspA1I NaeI NgoMIV
NspI PflMI PfoI PvuII SapI ScaI SfcI SmlI
SphI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII Bme1580I BmgBI BmtI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI DraI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI FseI FspI FspAI KasI KpnI MfeI MluI
MslI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI ZraI