Restriction map for
pF1KB6691
(Linear) (Six-base) MAPSORT of: KIBB6691.seq Check: 1983 from: 1 to: 1284
KIBB6691.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 507 1284
Size: 507 777
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 335 431 907 1003 1284
Size: 31 304 96 476 96 281
Fragments arranged by size:
476 304 281 96 96 31
AleI CACnn'nnGTG
Cuts at: 0 620 1284
Size: 620 664
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1027 1059 1284
Size: 1027 32 225
Fragments arranged by size:
1027 225 32
AlwNI CAG_nnn'CTG
Cuts at: 0 915 1284
Size: 915 369
ApaI G_GGCC'C
Cuts at: 0 656 1284
Size: 656 628
AvaI C'yCGr_G
Cuts at: 0 160 1284
Size: 160 1124
BamHI G'GATC_C
Cuts at: 0 99 1284
Size: 99 1185
BanII G_rGCy'C
Cuts at: 0 656 704 1284
Size: 656 48 580
Fragments arranged by size:
656 580 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 318 1284
Size: 318 966
BbvCI CC'TCA_GC
Cuts at: 0 757 1284
Size: 757 527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1002 1036 1284
Size: 1002 34 248
Fragments arranged by size:
1002 248 34
Bme1580I G_kGCm'C
Cuts at: 0 656 1284
Size: 656 628
BmrI ACTGGGnnnn_n'
Cuts at: 0 590 1284
Size: 590 694
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 718 1284
Size: 103 615 566
Fragments arranged by size:
615 566 103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 406 1284
Size: 278 128 878
Fragments arranged by size:
878 278 128
Bpu10I CC'TnA_GC
Cuts at: 0 757 1284
Size: 757 527
BsaHI Gr'CG_yC
Cuts at: 0 137 1265 1284
Size: 137 1128 19
Fragments arranged by size:
1128 137 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 712 754 1284
Size: 712 42 530
Fragments arranged by size:
712 530 42
BsiEI CG_ry'CG
Cuts at: 0 150 1284
Size: 150 1134
BsiHKAI G_wGCw'C
Cuts at: 0 348 704 992 1284
Size: 348 356 288 292
Fragments arranged by size:
356 348 292 288
BsmI GAATG_Cn'
Cuts at: 0 262 1264 1284
Size: 262 1002 20
Fragments arranged by size:
1002 262 20
Bsp1286I G_dGCh'C
Cuts at: 0 348 656 704 992 1284
Size: 348 308 48 288 292
Fragments arranged by size:
348 308 292 288 48
BspHI T'CATG_A
Cuts at: 0 461 1284
Size: 461 823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 507 900 1284
Size: 507 393 384
Fragments arranged by size:
507 393 384
BsrBI CCG'CTC
Cuts at: 0 95 1284
Size: 95 1189
BsrDI GCAATG_nn'
Cuts at: 0 1172 1284
Size: 1172 112
BssHII G'CGCG_C
Cuts at: 0 54 1284
Size: 54 1230
BstYI r'GATC_y
Cuts at: 0 99 1284
Size: 99 1185
Bsu36I CC'TnA_GG
Cuts at: 0 430 1284
Size: 430 854
BtgI C'CryG_G
Cuts at: 0 1001 1061 1284
Size: 1001 60 223
Fragments arranged by size:
1001 223 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 195 1284
Size: 195 1089
DraIII CAC_nnn'GTG
Cuts at: 0 119 1284
Size: 119 1165
EaeI y'GGCC_r
Cuts at: 0 238 917 1284
Size: 238 679 367
Fragments arranged by size:
679 367 238
EarI CTCTTCn'nnn_
Cuts at: 0 94 1284
Size: 94 1190
EcoICRI GAG'CTC
Cuts at: 0 702 1284
Size: 702 582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 278 335 406 431 907 1003 1284
Size: 31 247 57 71 25 476 96 281
Fragments arranged by size:
476 281 247 96 71 57 31 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1030 1284
Size: 1030 254
EcoO109I rG'GnC_Cy
Cuts at: 0 426 653 1284
Size: 426 227 631
Fragments arranged by size:
631 426 227
EcoRV GAT'ATC
Cuts at: 0 289 966 1284
Size: 289 677 318
Fragments arranged by size:
677 318 289
HaeII r_GCGC'y
Cuts at: 0 747 1284
Size: 747 537
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 1284
Size: 373 32 879
Fragments arranged by size:
879 373 32
HindIII A'AGCT_T
Cuts at: 0 233 364 1284
Size: 233 131 920
Fragments arranged by size:
920 233 131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 1284
Size: 1010 274
MscI TGG'CCA
Cuts at: 0 240 919 1284
Size: 240 679 365
Fragments arranged by size:
679 365 240
MslI CAynn'nnrTG
Cuts at: 0 620 688 1284
Size: 620 68 596
Fragments arranged by size:
620 596 68
MspA1I CmG'CkG
Cuts at: 0 9 69 152 492 620 756 981 1284
Size: 9 60 83 340 128 136 225 303
Fragments arranged by size:
340 303 225 136 128 83 60 9
NcoI C'CATG_G
Cuts at: 0 1001 1061 1284
Size: 1001 60 223
Fragments arranged by size:
1001 223 60
NspI r_CATG'y
Cuts at: 0 1155 1284
Size: 1155 129
PspOMI G'GGCC_C
Cuts at: 0 652 1284
Size: 652 632
PstI C_TGCA'G
Cuts at: 0 1139 1284
Size: 1139 145
PvuII CAG'CTG
Cuts at: 0 492 620 756 981 1284
Size: 492 128 136 225 303
Fragments arranged by size:
492 303 225 136 128
SacI G_AGCT'C
Cuts at: 0 704 1284
Size: 704 580
ScaI AGT'ACT
Cuts at: 0 769 1284
Size: 769 515
SfcI C'TryA_G
Cuts at: 0 210 1135 1284
Size: 210 925 149
Fragments arranged by size:
925 210 149
SspI AAT'ATT
Cuts at: 0 396 1284
Size: 396 888
StyI C'CwwG_G
Cuts at: 0 104 1001 1061 1284
Size: 104 897 60 223
Fragments arranged by size:
897 223 104 60
TatI w'GTAC_w
Cuts at: 0 767 1284
Size: 767 517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 714 1284
Size: 714 570
XmnI GAAnn'nnTTC
Cuts at: 0 956 1284
Size: 956 328
Enzymes that do cut:
AarI AcuI AleI AloI AlwNI ApaI AvaI BamHI
BanII BbsI BbvCI BcgI Bme1580I BmrI BplI BpmI
Bpu10I BsaHI BseRI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BssHII BstYI Bsu36I BtgI BtgZI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI
NspI PspOMI PstI PvuII SacI ScaI SfcI SspI
StyI TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BanI BbeI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BseYI BsgI BsiWI BsmBI BspEI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtsI ClaI
CspCI DraI DrdI EagI EciI EcoRI FalI FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI