Restriction map for pF1KB6691
 (Linear) (Six-base) MAPSORT of: KIBB6691.seq  Check: 1983  from: 1  to: 1284



KIBB6691.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     507    1284 
   Size:        507     777
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     335     431     907    1003    1284 
   Size:         31     304      96     476      96     281

  Fragments arranged by size: 

                 476     304     281      96      96      31
 
AleI CACnn'nnGTG

Cuts at:      0     620    1284 
   Size:        620     664
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1027    1059    1284 
   Size:       1027      32     225

  Fragments arranged by size: 

                1027     225      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     915    1284 
   Size:        915     369
 
ApaI G_GGCC'C

Cuts at:      0     656    1284 
   Size:        656     628
 
AvaI C'yCGr_G

Cuts at:      0     160    1284 
   Size:        160    1124
 
BamHI G'GATC_C

Cuts at:      0      99    1284 
   Size:         99    1185
 
BanII G_rGCy'C

Cuts at:      0     656     704    1284 
   Size:        656      48     580

  Fragments arranged by size: 

                 656     580      48
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     318    1284 
   Size:        318     966
 
BbvCI CC'TCA_GC

Cuts at:      0     757    1284 
   Size:        757     527
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1002    1036    1284 
   Size:       1002      34     248

  Fragments arranged by size: 

                1002     248      34
 
Bme1580I G_kGCm'C

Cuts at:      0     656    1284 
   Size:        656     628
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     590    1284 
   Size:        590     694
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     103     718    1284 
   Size:        103     615     566

  Fragments arranged by size: 

                 615     566     103
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     406    1284 
   Size:        278     128     878

  Fragments arranged by size: 

                 878     278     128
 
Bpu10I CC'TnA_GC

Cuts at:      0     757    1284 
   Size:        757     527
 
BsaHI Gr'CG_yC

Cuts at:      0     137    1265    1284 
   Size:        137    1128      19

  Fragments arranged by size: 

                1128     137      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     712     754    1284 
   Size:        712      42     530

  Fragments arranged by size: 

                 712     530      42
 
BsiEI CG_ry'CG

Cuts at:      0     150    1284 
   Size:        150    1134
 
BsiHKAI G_wGCw'C

Cuts at:      0     348     704     992    1284 
   Size:        348     356     288     292

  Fragments arranged by size: 

                 356     348     292     288
 
BsmI GAATG_Cn'

Cuts at:      0     262    1264    1284 
   Size:        262    1002      20

  Fragments arranged by size: 

                1002     262      20
 
Bsp1286I G_dGCh'C

Cuts at:      0     348     656     704     992    1284 
   Size:        348     308      48     288     292

  Fragments arranged by size: 

                 348     308     292     288      48
 
BspHI T'CATG_A

Cuts at:      0     461    1284 
   Size:        461     823
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     507     900    1284 
   Size:        507     393     384

  Fragments arranged by size: 

                 507     393     384
 
BsrBI CCG'CTC

Cuts at:      0      95    1284 
   Size:         95    1189
 
BsrDI GCAATG_nn'

Cuts at:      0    1172    1284 
   Size:       1172     112
 
BssHII G'CGCG_C

Cuts at:      0      54    1284 
   Size:         54    1230
 
BstYI r'GATC_y

Cuts at:      0      99    1284 
   Size:         99    1185
 
Bsu36I CC'TnA_GG

Cuts at:      0     430    1284 
   Size:        430     854
 
BtgI C'CryG_G

Cuts at:      0    1001    1061    1284 
   Size:       1001      60     223

  Fragments arranged by size: 

                1001     223      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     195    1284 
   Size:        195    1089
 
DraIII CAC_nnn'GTG

Cuts at:      0     119    1284 
   Size:        119    1165
 
EaeI y'GGCC_r

Cuts at:      0     238     917    1284 
   Size:        238     679     367

  Fragments arranged by size: 

                 679     367     238
 
EarI CTCTTCn'nnn_

Cuts at:      0      94    1284 
   Size:         94    1190
 
EcoICRI GAG'CTC

Cuts at:      0     702    1284 
   Size:        702     582
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     278     335     406     431     907    1003    1284 
   Size:         31     247      57      71      25     476      96     281

  Fragments arranged by size: 

                 476     281     247      96      71      57      31      25
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1030    1284 
   Size:       1030     254
 
EcoO109I rG'GnC_Cy

Cuts at:      0     426     653    1284 
   Size:        426     227     631

  Fragments arranged by size: 

                 631     426     227
 
EcoRV GAT'ATC

Cuts at:      0     289     966    1284 
   Size:        289     677     318

  Fragments arranged by size: 

                 677     318     289
 
HaeII r_GCGC'y

Cuts at:      0     747    1284 
   Size:        747     537
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     373     405    1284 
   Size:        373      32     879

  Fragments arranged by size: 

                 879     373      32
 
HindIII A'AGCT_T

Cuts at:      0     233     364    1284 
   Size:        233     131     920

  Fragments arranged by size: 

                 920     233     131
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1010    1284 
   Size:       1010     274
 
MscI TGG'CCA

Cuts at:      0     240     919    1284 
   Size:        240     679     365

  Fragments arranged by size: 

                 679     365     240
 
MslI CAynn'nnrTG

Cuts at:      0     620     688    1284 
   Size:        620      68     596

  Fragments arranged by size: 

                 620     596      68
 
MspA1I CmG'CkG

Cuts at:      0       9      69     152     492     620     756     981    1284 
   Size:          9      60      83     340     128     136     225     303

  Fragments arranged by size: 

                 340     303     225     136     128      83      60       9
            
 
NcoI C'CATG_G

Cuts at:      0    1001    1061    1284 
   Size:       1001      60     223

  Fragments arranged by size: 

                1001     223      60
 
NspI r_CATG'y

Cuts at:      0    1155    1284 
   Size:       1155     129
 
PspOMI G'GGCC_C

Cuts at:      0     652    1284 
   Size:        652     632
 
PstI C_TGCA'G

Cuts at:      0    1139    1284 
   Size:       1139     145
 
PvuII CAG'CTG

Cuts at:      0     492     620     756     981    1284 
   Size:        492     128     136     225     303

  Fragments arranged by size: 

                 492     303     225     136     128
 
SacI G_AGCT'C

Cuts at:      0     704    1284 
   Size:        704     580
 
ScaI AGT'ACT

Cuts at:      0     769    1284 
   Size:        769     515
 
SfcI C'TryA_G

Cuts at:      0     210    1135    1284 
   Size:        210     925     149

  Fragments arranged by size: 

                 925     210     149
 
SspI AAT'ATT

Cuts at:      0     396    1284 
   Size:        396     888
 
StyI C'CwwG_G

Cuts at:      0     104    1001    1061    1284 
   Size:        104     897      60     223

  Fragments arranged by size: 

                 897     223     104      60
 
TatI w'GTAC_w

Cuts at:      0     767    1284 
   Size:        767     517
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     714    1284 
   Size:        714     570
 
XmnI GAAnn'nnTTC

Cuts at:      0     956    1284 
   Size:        956     328
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AloI    AlwNI     ApaI     AvaI    BamHI
    BanII     BbsI    BbvCI     BcgI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BsaHI    BseRI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI   BssHII    BstYI   Bsu36I     BtgI    BtgZI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
    HaeII    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I     NcoI
     NspI   PspOMI     PstI    PvuII     SacI     ScaI     SfcI     SspI
     StyI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI     BbeI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI    BsiWI    BsmBI    BspEI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtsI     ClaI
    CspCI     DraI     DrdI     EagI     EciI    EcoRI     FalI     FseI
     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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