(Linear) (Six-base) MAPSORT of: KIBB6691.seq Check: 1983 from: 1 to: 1284 KIBB6691.orf With 182 enzymes: SgfI * October 7, 2009 14:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 507 1284 Size: 507 777 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 335 431 907 1003 1284 Size: 31 304 96 476 96 281 Fragments arranged by size: 476 304 281 96 96 31 AleI CACnn'nnGTG Cuts at: 0 620 1284 Size: 620 664 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1027 1059 1284 Size: 1027 32 225 Fragments arranged by size: 1027 225 32 AlwNI CAG_nnn'CTG Cuts at: 0 915 1284 Size: 915 369 ApaI G_GGCC'C Cuts at: 0 656 1284 Size: 656 628 AvaI C'yCGr_G Cuts at: 0 160 1284 Size: 160 1124 BamHI G'GATC_C Cuts at: 0 99 1284 Size: 99 1185 BanII G_rGCy'C Cuts at: 0 656 704 1284 Size: 656 48 580 Fragments arranged by size: 656 580 48 BbsI GAAGACnn'nnnn_ Cuts at: 0 318 1284 Size: 318 966 BbvCI CC'TCA_GC Cuts at: 0 757 1284 Size: 757 527 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1002 1036 1284 Size: 1002 34 248 Fragments arranged by size: 1002 248 34 Bme1580I G_kGCm'C Cuts at: 0 656 1284 Size: 656 628 BmrI ACTGGGnnnn_n' Cuts at: 0 590 1284 Size: 590 694 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 103 718 1284 Size: 103 615 566 Fragments arranged by size: 615 566 103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 278 406 1284 Size: 278 128 878 Fragments arranged by size: 878 278 128 Bpu10I CC'TnA_GC Cuts at: 0 757 1284 Size: 757 527 BsaHI Gr'CG_yC Cuts at: 0 137 1265 1284 Size: 137 1128 19 Fragments arranged by size: 1128 137 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 712 754 1284 Size: 712 42 530 Fragments arranged by size: 712 530 42 BsiEI CG_ry'CG Cuts at: 0 150 1284 Size: 150 1134 BsiHKAI G_wGCw'C Cuts at: 0 348 704 992 1284 Size: 348 356 288 292 Fragments arranged by size: 356 348 292 288 BsmI GAATG_Cn' Cuts at: 0 262 1264 1284 Size: 262 1002 20 Fragments arranged by size: 1002 262 20 Bsp1286I G_dGCh'C Cuts at: 0 348 656 704 992 1284 Size: 348 308 48 288 292 Fragments arranged by size: 348 308 292 288 48 BspHI T'CATG_A Cuts at: 0 461 1284 Size: 461 823 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 507 900 1284 Size: 507 393 384 Fragments arranged by size: 507 393 384 BsrBI CCG'CTC Cuts at: 0 95 1284 Size: 95 1189 BsrDI GCAATG_nn' Cuts at: 0 1172 1284 Size: 1172 112 BssHII G'CGCG_C Cuts at: 0 54 1284 Size: 54 1230 BstYI r'GATC_y Cuts at: 0 99 1284 Size: 99 1185 Bsu36I CC'TnA_GG Cuts at: 0 430 1284 Size: 430 854 BtgI C'CryG_G Cuts at: 0 1001 1061 1284 Size: 1001 60 223 Fragments arranged by size: 1001 223 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 195 1284 Size: 195 1089 DraIII CAC_nnn'GTG Cuts at: 0 119 1284 Size: 119 1165 EaeI y'GGCC_r Cuts at: 0 238 917 1284 Size: 238 679 367 Fragments arranged by size: 679 367 238 EarI CTCTTCn'nnn_ Cuts at: 0 94 1284 Size: 94 1190 EcoICRI GAG'CTC Cuts at: 0 702 1284 Size: 702 582 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 278 335 406 431 907 1003 1284 Size: 31 247 57 71 25 476 96 281 Fragments arranged by size: 476 281 247 96 71 57 31 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 1030 1284 Size: 1030 254 EcoO109I rG'GnC_Cy Cuts at: 0 426 653 1284 Size: 426 227 631 Fragments arranged by size: 631 426 227 EcoRV GAT'ATC Cuts at: 0 289 966 1284 Size: 289 677 318 Fragments arranged by size: 677 318 289 HaeII r_GCGC'y Cuts at: 0 747 1284 Size: 747 537 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 373 405 1284 Size: 373 32 879 Fragments arranged by size: 879 373 32 HindIII A'AGCT_T Cuts at: 0 233 364 1284 Size: 233 131 920 Fragments arranged by size: 920 233 131 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1010 1284 Size: 1010 274 MscI TGG'CCA Cuts at: 0 240 919 1284 Size: 240 679 365 Fragments arranged by size: 679 365 240 MslI CAynn'nnrTG Cuts at: 0 620 688 1284 Size: 620 68 596 Fragments arranged by size: 620 596 68 MspA1I CmG'CkG Cuts at: 0 9 69 152 492 620 756 981 1284 Size: 9 60 83 340 128 136 225 303 Fragments arranged by size: 340 303 225 136 128 83 60 9 NcoI C'CATG_G Cuts at: 0 1001 1061 1284 Size: 1001 60 223 Fragments arranged by size: 1001 223 60 NspI r_CATG'y Cuts at: 0 1155 1284 Size: 1155 129 PspOMI G'GGCC_C Cuts at: 0 652 1284 Size: 652 632 PstI C_TGCA'G Cuts at: 0 1139 1284 Size: 1139 145 PvuII CAG'CTG Cuts at: 0 492 620 756 981 1284 Size: 492 128 136 225 303 Fragments arranged by size: 492 303 225 136 128 SacI G_AGCT'C Cuts at: 0 704 1284 Size: 704 580 ScaI AGT'ACT Cuts at: 0 769 1284 Size: 769 515 SfcI C'TryA_G Cuts at: 0 210 1135 1284 Size: 210 925 149 Fragments arranged by size: 925 210 149 SspI AAT'ATT Cuts at: 0 396 1284 Size: 396 888 StyI C'CwwG_G Cuts at: 0 104 1001 1061 1284 Size: 104 897 60 223 Fragments arranged by size: 897 223 104 60 TatI w'GTAC_w Cuts at: 0 767 1284 Size: 767 517 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 714 1284 Size: 714 570 XmnI GAAnn'nnTTC Cuts at: 0 956 1284 Size: 956 328 Enzymes that do cut: AarI AcuI AleI AloI AlwNI ApaI AvaI BamHI BanII BbsI BbvCI BcgI Bme1580I BmrI BplI BpmI Bpu10I BsaHI BseRI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssHII BstYI Bsu36I BtgI BtgZI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI NspI PspOMI PstI PvuII SacI ScaI SfcI SspI StyI TatI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiWI BsmBI BspEI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI DraI DrdI EagI EciI EcoRI FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI