Restriction map for
pF1KB6687
(Linear) (Six-base) MAPSORT of: KIBB6687.seq Check: 1969 from: 1 to: 1281
KIBB6687.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AclI AA'CG_TT
Cuts at: 0 614 1281
Size: 614 667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1281
Size: 1004 277
AhdI GACnn_n'nnGTC
Cuts at: 0 624 1281
Size: 624 657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 360 1281
Size: 360 921
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 717 749 1281
Size: 717 32 532
Fragments arranged by size:
717 532 32
AlwNI CAG_nnn'CTG
Cuts at: 0 405 1258 1281
Size: 405 853 23
Fragments arranged by size:
853 405 23
ApoI r'AATT_y
Cuts at: 0 81 298 739 1281
Size: 81 217 441 542
Fragments arranged by size:
542 441 217 81
AvaI C'yCGr_G
Cuts at: 0 580 1281
Size: 580 701
BanII G_rGCy'C
Cuts at: 0 108 329 1281
Size: 108 221 952
Fragments arranged by size:
952 221 108
BbsI GAAGACnn'nnnn_
Cuts at: 0 1190 1281
Size: 1190 91
BclI T'GATC_A
Cuts at: 0 653 693 1148 1281
Size: 653 40 455 133
Fragments arranged by size:
653 455 133 40
BfrBI ATG'CAT
Cuts at: 0 916 1281
Size: 916 365
BglI GCCn_nnn'nGGC
Cuts at: 0 205 877 1281
Size: 205 672 404
Fragments arranged by size:
672 404 205
BlpI GC'TnA_GC
Cuts at: 0 169 1281
Size: 169 1112
BmrI ACTGGGnnnn_n'
Cuts at: 0 455 1281
Size: 455 826
Bpu10I CC'TnA_GC
Cuts at: 0 99 1281
Size: 99 1182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1281
Size: 92 1189
BsaBI GATnn'nnATC
Cuts at: 0 338 1281
Size: 338 943
BsaHI Gr'CG_yC
Cuts at: 0 547 1281
Size: 547 734
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1105 1281
Size: 1105 176
BseYI C'CCAG_C
Cuts at: 0 840 1258 1281
Size: 840 418 23
Fragments arranged by size:
840 418 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1160 1281
Size: 1160 121
BsiHKAI G_wGCw'C
Cuts at: 0 108 329 579 1281
Size: 108 221 250 702
Fragments arranged by size:
702 250 221 108
Bsp1286I G_dGCh'C
Cuts at: 0 108 329 579 1281
Size: 108 221 250 702
Fragments arranged by size:
702 250 221 108
BspHI T'CATG_A
Cuts at: 0 372 743 1281
Size: 372 371 538
Fragments arranged by size:
538 372 371
BstXI CCAn_nnnn'nTGG
Cuts at: 0 903 1281
Size: 903 378
BstYI r'GATC_y
Cuts at: 0 926 1007 1212 1281
Size: 926 81 205 69
Fragments arranged by size:
926 205 81 69
BtsI GCAGTG_nn'
Cuts at: 0 1151 1281
Size: 1151 130
DraI TTT'AAA
Cuts at: 0 86 1281
Size: 86 1195
EaeI y'GGCC_r
Cuts at: 0 515 1281
Size: 515 766
EarI CTCTTCn'nnn_
Cuts at: 0 826 1043 1281
Size: 826 217 238
Fragments arranged by size:
826 238 217
EcoICRI GAG'CTC
Cuts at: 0 106 327 1281
Size: 106 221 954
Fragments arranged by size:
954 221 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1281
Size: 1004 277
EcoRI G'AATT_C
Cuts at: 0 739 1281
Size: 739 542
EcoRV GAT'ATC
Cuts at: 0 76 1281
Size: 76 1205
FspI TGC'GCA
Cuts at: 0 123 1281
Size: 123 1158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 610 638 642 670 877 909 1281
Size: 610 28 4 28 207 32 372
Fragments arranged by size:
610 372 207 32 28 28 4
HincII GTy'rAC
Cuts at: 0 618 1208 1281
Size: 618 590 73
Fragments arranged by size:
618 590 73
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 972 1240 1281
Size: 972 268 41
Fragments arranged by size:
972 268 41
MscI TGG'CCA
Cuts at: 0 517 1281
Size: 517 764
MslI CAynn'nnrTG
Cuts at: 0 319 1281
Size: 319 962
MspA1I CmG'CkG
Cuts at: 0 844 1258 1281
Size: 844 414 23
Fragments arranged by size:
844 414 23
NsiI A_TGCA'T
Cuts at: 0 918 1281
Size: 918 363
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 206 238 1281
Size: 206 32 1043
Fragments arranged by size:
1043 206 32
PshAI GACnn'nnGTC
Cuts at: 0 509 1241 1281
Size: 509 732 40
Fragments arranged by size:
732 509 40
PstI C_TGCA'G
Cuts at: 0 528 1281
Size: 528 753
PvuII CAG'CTG
Cuts at: 0 844 1258 1281
Size: 844 414 23
Fragments arranged by size:
844 414 23
SacI G_AGCT'C
Cuts at: 0 108 329 1281
Size: 108 221 952
Fragments arranged by size:
952 221 108
SfcI C'TryA_G
Cuts at: 0 92 210 524 570 932 1275 1281
Size: 92 118 314 46 362 343 6
Fragments arranged by size:
362 343 314 118 92 46 6
SmlI C'TyrA_G
Cuts at: 0 107 1281
Size: 107 1174
StyI C'CwwG_G
Cuts at: 0 591 1281
Size: 591 690
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 499 623 1281
Size: 499 124 658
Fragments arranged by size:
658 499 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 992 1024 1281
Size: 992 32 257
Fragments arranged by size:
992 257 32
XbaI T'CTAG_A
Cuts at: 0 1245 1281
Size: 1245 36
XmnI GAAnn'nnTTC
Cuts at: 0 271 299 820 1281
Size: 271 28 521 461
Fragments arranged by size:
521 461 271 28
Enzymes that do cut:
AclI AcuI AhdI AlfI AloI AlwNI ApoI AvaI
BanII BbsI BclI BfrBI BglI BlpI BmrI Bpu10I
BpuEI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BstXI BstYI BtsI DraI EaeI EarI EcoICRI
Eco57MI EcoRI EcoRV FspI Hin4I HincII MmeI MscI
MslI MspA1I NsiI PpiI PshAI PstI PvuII SacI
SfcI SmlI StyI TaqII TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AleI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BglII Bme1580I
BmgBI BmtI BplI BpmI BsaI BsaAI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoNI EcoO109I FalI FseI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XcmI XhoI XmaI ZraI