Restriction map for
pF1KB6686
(Linear) (Six-base) MAPSORT of: KIBB6686.seq Check: 5706 from: 1 to: 1374
KIBB6686.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 0 922 1374
Size: 922 452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 552 1374
Size: 442 110 822
Fragments arranged by size:
822 442 110
AflIII A'CryG_T
Cuts at: 0 1186 1374
Size: 1186 188
ApoI r'AATT_y
Cuts at: 0 745 1374
Size: 745 629
AvaI C'yCGr_G
Cuts at: 0 1225 1374
Size: 1225 149
BanI G'GyrC_C
Cuts at: 0 416 1374
Size: 416 958
BanII G_rGCy'C
Cuts at: 0 22 433 485 653 971 1130 1374
Size: 22 411 52 168 318 159 244
Fragments arranged by size:
411 318 244 168 159 52 22
BbvCI CC'TCA_GC
Cuts at: 0 875 1263 1374
Size: 875 388 111
Fragments arranged by size:
875 388 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1206 1240 1374
Size: 1206 34 134
Fragments arranged by size:
1206 134 34
BglI GCCn_nnn'nGGC
Cuts at: 0 553 1374
Size: 553 821
BlpI GC'TnA_GC
Cuts at: 0 1015 1120 1374
Size: 1015 105 254
Fragments arranged by size:
1015 254 105
BmgBI CAC'GTC
Cuts at: 0 1189 1374
Size: 1189 185
BmtI G_CTAG'C
Cuts at: 0 440 709 1374
Size: 440 269 665
Fragments arranged by size:
665 440 269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 394 415 553 694 1012 1054 1213
Size: 376 18 21 138 141 318 42 159
Cuts at: 1213 1374
Size: 161
Fragments arranged by size:
376 318 161 159 141 138 42 21
18
Bpu10I CC'TnA_GC
Cuts at: 0 443 875 970 1263 1374
Size: 443 432 95 293 111
Fragments arranged by size:
443 432 293 111 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1374
Size: 559 815
BsaBI GATnn'nnATC
Cuts at: 0 682 1374
Size: 682 692
BsaHI Gr'CG_yC
Cuts at: 0 1238 1374
Size: 1238 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 128 158 1374
Size: 128 30 1216
Fragments arranged by size:
1216 128 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 12 86 286 391 748 1374
Size: 12 74 200 105 357 626
Fragments arranged by size:
626 357 200 105 74 12
BseYI C'CCAG_C
Cuts at: 0 66 124 432 1010 1115 1374
Size: 66 58 308 578 105 259
Fragments arranged by size:
578 308 259 105 66 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 1374
Size: 920 454
BsiEI CG_ry'CG
Cuts at: 0 339 1374
Size: 339 1035
BsiHKAI G_wGCw'C
Cuts at: 0 22 485 653 971 1130 1374
Size: 22 463 168 318 159 244
Fragments arranged by size:
463 318 244 168 159 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1045 1374
Size: 1045 329
Bsp1286I G_dGCh'C
Cuts at: 0 22 433 485 653 971 1130 1374
Size: 22 411 52 168 318 159 244
Fragments arranged by size:
411 318 244 168 159 52 22
BsrBI CCG'CTC
Cuts at: 0 447 1374
Size: 447 927
BsrDI GCAATG_nn'
Cuts at: 0 312 1374
Size: 312 1062
BssHII G'CGCG_C
Cuts at: 0 366 1374
Size: 366 1008
BstYI r'GATC_y
Cuts at: 0 404 494 1085 1374
Size: 404 90 591 289
Fragments arranged by size:
591 404 289 90
BtgI C'CryG_G
Cuts at: 0 78 848 1374
Size: 78 770 526
Fragments arranged by size:
770 526 78
BtsI GCAGTG_nn'
Cuts at: 0 929 1140 1374
Size: 929 211 234
Fragments arranged by size:
929 234 211
EaeI y'GGCC_r
Cuts at: 0 765 1374
Size: 765 609
EarI CTCTTCn'nnn_
Cuts at: 0 473 695 725 1374
Size: 473 222 30 649
Fragments arranged by size:
649 473 222 30
EcoICRI GAG'CTC
Cuts at: 0 20 483 651 969 1128 1374
Size: 20 463 168 318 159 246
Fragments arranged by size:
463 318 246 168 159 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 394 415 442 552 553 694 1012
Size: 376 18 21 27 110 1 141 318
Cuts at: 1012 1054 1213 1374
Size: 42 159 161
Fragments arranged by size:
376 318 161 159 141 110 42 27
21 18 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 358 376 1225 1374
Size: 358 18 849 149
Fragments arranged by size:
849 358 149 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 991 1023 1374
Size: 991 32 351
Fragments arranged by size:
991 351 32
HincII GTy'rAC
Cuts at: 0 270 774 1374
Size: 270 504 600
Fragments arranged by size:
600 504 270
MslI CAynn'nnrTG
Cuts at: 0 934 1374
Size: 934 440
MspA1I CmG'CkG
Cuts at: 0 66 124 1014 1077 1119 1374
Size: 66 58 890 63 42 255
Fragments arranged by size:
890 255 66 63 58 42
NcoI C'CATG_G
Cuts at: 0 848 1374
Size: 848 526
NheI G'CTAG_C
Cuts at: 0 436 705 1374
Size: 436 269 669
Fragments arranged by size:
669 436 269
PflMI CCAn_nnn'nTGG
Cuts at: 0 609 990 1374
Size: 609 381 384
Fragments arranged by size:
609 384 381
PfoI T'CCnGG_A
Cuts at: 0 485 530 1374
Size: 485 45 844
Fragments arranged by size:
844 485 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 868 900 1374
Size: 868 32 474
Fragments arranged by size:
868 474 32
PspXI vC'TCGA_Gb
Cuts at: 0 1225 1374
Size: 1225 149
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1374
Size: 1144 32 198
Fragments arranged by size:
1144 198 32
PstI C_TGCA'G
Cuts at: 0 17 1007 1076 1082 1374
Size: 17 990 69 6 292
Fragments arranged by size:
990 292 69 17 6
PvuII CAG'CTG
Cuts at: 0 66 124 1014 1077 1119 1374
Size: 66 58 890 63 42 255
Fragments arranged by size:
890 255 66 63 58 42
SacI G_AGCT'C
Cuts at: 0 22 485 653 971 1130 1374
Size: 22 463 168 318 159 244
Fragments arranged by size:
463 318 244 168 159 22
SapI GCTCTTCn'nnn_
Cuts at: 0 473 695 1374
Size: 473 222 679
Fragments arranged by size:
679 473 222
SexAI A'CCwGG_T
Cuts at: 0 755 1374
Size: 755 619
SfcI C'TryA_G
Cuts at: 0 13 453 1003 1072 1078 1374
Size: 13 440 550 69 6 296
Fragments arranged by size:
550 440 296 69 13 6
SmlI C'TyrA_G
Cuts at: 0 574 1225 1374
Size: 574 651 149
Fragments arranged by size:
651 574 149
StuI AGG'CCT
Cuts at: 0 989 1371 1374
Size: 989 382 3
Fragments arranged by size:
989 382 3
StyI C'CwwG_G
Cuts at: 0 204 228 848 984 1374
Size: 204 24 620 136 390
Fragments arranged by size:
620 390 204 136 24
TatI w'GTAC_w
Cuts at: 0 1160 1374
Size: 1160 214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 128 160 1374
Size: 128 32 1214
Fragments arranged by size:
1214 128 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 390 555 1374
Size: 390 165 819
Fragments arranged by size:
819 390 165
XhoI C'TCGA_G
Cuts at: 0 1225 1374
Size: 1225 149
Enzymes that do cut:
AccI AcuI AflIII ApoI AvaI BanI BanII BbvCI
BcgI BglI BlpI BmgBI BmtI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BsrBI BsrDI BssHII BstYI BtgI BtsI
EaeI EarI EcoICRI Eco57MI EcoNI Hin4I HincII MslI
MspA1I NcoI NheI PflMI PfoI PpiI PspXI PsrI
PstI PvuII SacI SapI SexAI SfcI SmlI StuI
StyI TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BciVI BclI
BfrBI BglII Bme1580I BmrI BplI BsaI BsaAI BsaWI
BsiWI BsmI BspEI BspHI BspMI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XbaI XmaI XmnI ZraI