Restriction map for
pF1KB6680
(Linear) (Six-base) MAPSORT of: KIBB6680.seq Check: 9918 from: 1 to: 1368
KIBB6680.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
Acc65I G'GTAC_C
Cuts at: 0 419 1368
Size: 419 949
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 555 739 876 1262 1368
Size: 143 412 184 137 386 106
Fragments arranged by size:
412 386 184 143 137 106
AlwNI CAG_nnn'CTG
Cuts at: 0 993 1368
Size: 993 375
AvaI C'yCGr_G
Cuts at: 0 53 524 1228 1368
Size: 53 471 704 140
Fragments arranged by size:
704 471 140 53
BanI G'GyrC_C
Cuts at: 0 419 1368
Size: 419 949
BanII G_rGCy'C
Cuts at: 0 488 1100 1133 1368
Size: 488 612 33 235
Fragments arranged by size:
612 488 235 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1209 1243 1368
Size: 1209 34 125
Fragments arranged by size:
1209 125 34
BglI GCCn_nnn'nGGC
Cuts at: 0 556 1249 1368
Size: 556 693 119
Fragments arranged by size:
693 556 119
BlpI GC'TnA_GC
Cuts at: 0 1018 1368
Size: 1018 350
Bme1580I G_kGCm'C
Cuts at: 0 375 1368
Size: 375 993
BmtI G_CTAG'C
Cuts at: 0 712 1368
Size: 712 656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 942 1368
Size: 942 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 418 556 697 1015 1057 1132 1216
Size: 397 21 138 141 318 42 75 84
Cuts at: 1216 1368
Size: 152
Fragments arranged by size:
397 318 152 141 138 84 75 42
21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 1368
Size: 562 806
BsaI GGTCTCn'nnnn_
Cuts at: 0 301 1368
Size: 301 1067
BsaAI yAC'GTr
Cuts at: 0 370 1368
Size: 370 998
BsaBI GATnn'nnATC
Cuts at: 0 685 1368
Size: 685 683
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 236 266 1368
Size: 236 30 1102
Fragments arranged by size:
1102 236 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 185 289 394 718 751 919 1368
Size: 185 104 105 324 33 168 449
Fragments arranged by size:
449 324 185 168 105 104 33
BseYI C'CCAG_C
Cuts at: 0 73 435 1013 1030 1118 1368
Size: 73 362 578 17 88 250
Fragments arranged by size:
578 362 250 88 73 17
BsiHKAI G_wGCw'C
Cuts at: 0 488 1133 1368
Size: 488 645 235
Fragments arranged by size:
645 488 235
BsmI GAATG_Cn'
Cuts at: 0 454 1368
Size: 454 914
BsmBI CGTCTCn'nnnn_
Cuts at: 0 969 1368
Size: 969 399
Bsp1286I G_dGCh'C
Cuts at: 0 375 488 1100 1133 1368
Size: 375 113 612 33 235
Fragments arranged by size:
612 375 235 113 33
BsrDI GCAATG_nn'
Cuts at: 0 315 1368
Size: 315 1053
BsrFI r'CCGG_y
Cuts at: 0 766 1368
Size: 766 602
BstYI r'GATC_y
Cuts at: 0 407 1088 1368
Size: 407 681 280
Fragments arranged by size:
681 407 280
BtgI C'CryG_G
Cuts at: 0 851 1368
Size: 851 517
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 302 1368
Size: 302 1066
DraIII CAC_nnn'GTG
Cuts at: 0 802 1368
Size: 802 566
EaeI y'GGCC_r
Cuts at: 0 503 662 764 768 1049 1368
Size: 503 159 102 4 281 319
Fragments arranged by size:
503 319 281 159 102 4
EarI CTCTTCn'nnn_
Cuts at: 0 162 476 728 1281 1316 1368
Size: 162 314 252 553 35 52
Fragments arranged by size:
553 314 252 162 52 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 871 1368
Size: 871 497
EcoICRI GAG'CTC
Cuts at: 0 486 1131 1368
Size: 486 645 237
Fragments arranged by size:
645 486 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 397 418 555 556 697 739 876
Size: 143 254 21 137 1 141 42 137
Cuts at: 876 1015 1057 1132 1216 1262 1368
Size: 139 42 75 84 46 106
Fragments arranged by size:
254 143 141 139 137 137 106 84
75 46 42 42 21 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 379 604 700 1228 1368
Size: 361 18 225 96 528 140
Fragments arranged by size:
528 361 225 140 96 18
EcoO109I rG'GnC_Cy
Cuts at: 0 693 1368
Size: 693 675
FseI GG_CCGG'CC
Cuts at: 0 770 1368
Size: 770 598
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 994 1026 1134 1166 1368
Size: 994 32 108 32 202
Fragments arranged by size:
994 202 108 32 32
KpnI G_GTAC'C
Cuts at: 0 423 1368
Size: 423 945
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 953 1368
Size: 953 415
MscI TGG'CCA
Cuts at: 0 505 664 1368
Size: 505 159 704
Fragments arranged by size:
704 505 159
MslI CAynn'nnrTG
Cuts at: 0 937 1313 1368
Size: 937 376 55
Fragments arranged by size:
937 376 55
MspA1I CmG'CkG
Cuts at: 0 762 1080 1122 1368
Size: 762 318 42 246
Fragments arranged by size:
762 318 246 42
NaeI GCC'GGC
Cuts at: 0 768 1368
Size: 768 600
NcoI C'CATG_G
Cuts at: 0 851 1368
Size: 851 517
NgoMIV G'CCGG_C
Cuts at: 0 766 1368
Size: 766 602
NheI G'CTAG_C
Cuts at: 0 708 1368
Size: 708 660
PfoI T'CCnGG_A
Cuts at: 0 488 533 644 1368
Size: 488 45 111 724
Fragments arranged by size:
724 488 111 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 871 903 1368
Size: 871 32 465
Fragments arranged by size:
871 465 32
PspXI vC'TCGA_Gb
Cuts at: 0 1228 1368
Size: 1228 140
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1147 1179 1368
Size: 1147 32 189
Fragments arranged by size:
1147 189 32
PstI C_TGCA'G
Cuts at: 0 1010 1085 1368
Size: 1010 75 283
Fragments arranged by size:
1010 283 75
PvuII CAG'CTG
Cuts at: 0 762 1080 1122 1368
Size: 762 318 42 246
Fragments arranged by size:
762 318 246 42
SacI G_AGCT'C
Cuts at: 0 488 1133 1368
Size: 488 645 235
Fragments arranged by size:
645 488 235
SapI GCTCTTCn'nnn_
Cuts at: 0 476 1368
Size: 476 892
SfcI C'TryA_G
Cuts at: 0 456 1006 1081 1368
Size: 456 550 75 287
Fragments arranged by size:
550 456 287 75
SmaI CCC'GGG
Cuts at: 0 526 1368
Size: 526 842
SmlI C'TyrA_G
Cuts at: 0 577 1228 1368
Size: 577 651 140
Fragments arranged by size:
651 577 140
StuI AGG'CCT
Cuts at: 0 883 1368
Size: 883 485
StyI C'CwwG_G
Cuts at: 0 851 1368
Size: 851 517
TatI w'GTAC_w
Cuts at: 0 1163 1368
Size: 1163 205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 672 1099 1368
Size: 393 279 427 269
Fragments arranged by size:
427 393 279 269
XhoI C'TCGA_G
Cuts at: 0 1228 1368
Size: 1228 140
XmaI C'CCGG_G
Cuts at: 0 524 1368
Size: 524 844
XmnI GAAnn'nnTTC
Cuts at: 0 748 1368
Size: 748 620
Enzymes that do cut:
Acc65I AcuI AlwNI AvaI BanI BanII BcgI BglI
BlpI Bme1580I BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BsrDI BsrFI BstYI BtgI BtgZI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FseI Hin4I KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI
PfoI PpiI PspXI PsrI PstI PvuII SacI SapI
SfcI SmaI SmlI StuI StyI TatI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BmgBI BmrI Bpu10I BsaHI
BsaWI BsgI BsiEI BsiWI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DrdI EagI EcoRI
EcoRV FalI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI NarI NdeI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PsiI PspOMI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I
XbaI ZraI