Restriction map for
pF1KB6675
(Linear) (Six-base) MAPSORT of: KIBB6675.seq Check: 3903 from: 1 to: 1281
KIBB6675.orf
With 182 enzymes: SgfI *
March 22, 2013 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 676 1281
Size: 676 605
AleI CACnn'nnGTG
Cuts at: 0 119 1281
Size: 119 1162
AlwNI CAG_nnn'CTG
Cuts at: 0 939 1281
Size: 939 342
ApaLI G'TGCA_C
Cuts at: 0 255 537 1281
Size: 255 282 744
Fragments arranged by size:
744 282 255
AvaI C'yCGr_G
Cuts at: 0 393 731 972 1108 1281
Size: 393 338 241 136 173
Fragments arranged by size:
393 338 241 173 136
BanI G'GyrC_C
Cuts at: 0 10 79 288 648 736 1079 1281
Size: 10 69 209 360 88 343 202
Fragments arranged by size:
360 343 209 202 88 69 10
BanII G_rGCy'C
Cuts at: 0 197 275 394 613 1281
Size: 197 78 119 219 668
Fragments arranged by size:
668 219 197 119 78
BbeI G_GCGC'C
Cuts at: 0 292 1281
Size: 292 989
BglI GCCn_nnn'nGGC
Cuts at: 0 88 101 452 1204 1281
Size: 88 13 351 752 77
Fragments arranged by size:
752 351 88 77 13
Bme1580I G_kGCm'C
Cuts at: 0 259 448 541 1281
Size: 259 189 93 740
Fragments arranged by size:
740 259 189 93
BmgBI CAC'GTC
Cuts at: 0 225 1281
Size: 225 1056
BmrI ACTGGGnnnn_n'
Cuts at: 0 365 1281
Size: 365 916
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 408 916 1252 1281
Size: 408 508 336 29
Fragments arranged by size:
508 408 336 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 638 1281
Size: 259 379 643
Fragments arranged by size:
643 379 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 1281
Size: 970 311
BsaAI yAC'GTr
Cuts at: 0 458 480 1281
Size: 458 22 801
Fragments arranged by size:
801 458 22
BsaHI Gr'CG_yC
Cuts at: 0 289 311 554 877 1196 1281
Size: 289 22 243 323 319 85
Fragments arranged by size:
323 319 289 243 85 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1105 1135 1234 1264 1281
Size: 1105 30 99 30 17
Fragments arranged by size:
1105 99 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 454 580 1048 1281
Size: 454 126 468 233
Fragments arranged by size:
468 454 233 126
BseYI C'CCAG_C
Cuts at: 0 161 545 628 716 1020 1095 1166 1281
Size: 161 384 83 88 304 75 71 115
Fragments arranged by size:
384 304 161 115 88 83 75 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 1272 1281
Size: 483 789 9
Fragments arranged by size:
789 483 9
BsiEI CG_ry'CG
Cuts at: 0 21 239 1281
Size: 21 218 1042
Fragments arranged by size:
1042 218 21
BsiHKAI G_wGCw'C
Cuts at: 0 259 541 1115 1281
Size: 259 282 574 166
Fragments arranged by size:
574 282 259 166
BsmBI CGTCTCn'nnnn_
Cuts at: 0 343 1281
Size: 343 938
Bsp1286I G_dGCh'C
Cuts at: 0 197 259 275 394 448 541 613 1115
Size: 197 62 16 119 54 93 72 502
Cuts at: 1115 1281
Size: 166
Fragments arranged by size:
502 197 166 119 93 72 62 54
16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 676 1281
Size: 676 605
BsrFI r'CCGG_y
Cuts at: 0 16 860 1281
Size: 16 844 421
Fragments arranged by size:
844 421 16
BsrGI T'GTAC_A
Cuts at: 0 107 1281
Size: 107 1174
BssSI C'ACGA_G
Cuts at: 0 400 1237 1281
Size: 400 837 44
Fragments arranged by size:
837 400 44
BstAPI GCAn_nnn'nTGC
Cuts at: 0 137 254 263 506 1281
Size: 137 117 9 243 775
Fragments arranged by size:
775 243 137 117 9
BstEII G'GTnAC_C
Cuts at: 0 693 726 1281
Size: 693 33 555
Fragments arranged by size:
693 555 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 169 480 681 705 1281
Size: 169 311 201 24 576
Fragments arranged by size:
576 311 201 169 24
BstYI r'GATC_y
Cuts at: 0 569 1281
Size: 569 712
Bsu36I CC'TnA_GG
Cuts at: 0 644 1281
Size: 644 637
BtgI C'CryG_G
Cuts at: 0 26 722 923 1281
Size: 26 696 201 358
Fragments arranged by size:
696 358 201 26
BtsI GCAGTG_nn'
Cuts at: 0 1260 1262 1281
Size: 1260 2 19
Fragments arranged by size:
1260 19 2
DraIII CAC_nnn'GTG
Cuts at: 0 263 434 506 1281
Size: 263 171 72 775
Fragments arranged by size:
775 263 171 72
EaeI y'GGCC_r
Cuts at: 0 18 576 862 1205 1281
Size: 18 558 286 343 76
Fragments arranged by size:
558 343 286 76 18
EagI C'GGCC_G
Cuts at: 0 18 1281
Size: 18 1263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 638 1281
Size: 259 379 643
Fragments arranged by size:
643 379 259
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 816 1281
Size: 148 668 465
Fragments arranged by size:
668 465 148
EcoO109I rG'GnC_Cy
Cuts at: 0 981 1281
Size: 981 300
HaeII r_GCGC'y
Cuts at: 0 292 363 525 1187 1281
Size: 292 71 162 662 94
Fragments arranged by size:
662 292 162 94 71
HindIII A'AGCT_T
Cuts at: 0 1045 1281
Size: 1045 236
KasI G'GCGC_C
Cuts at: 0 288 1281
Size: 288 993
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 488 562 1281
Size: 245 243 74 719
Fragments arranged by size:
719 245 243 74
MslI CAynn'nnrTG
Cuts at: 0 119 703 922 1281
Size: 119 584 219 359
Fragments arranged by size:
584 359 219 119
MspA1I CmG'CkG
Cuts at: 0 119 323 830 1281
Size: 119 204 507 451
Fragments arranged by size:
507 451 204 119
NaeI GCC'GGC
Cuts at: 0 862 1281
Size: 862 419
NarI GG'CG_CC
Cuts at: 0 289 1281
Size: 289 992
NcoI C'CATG_G
Cuts at: 0 26 1281
Size: 26 1255
NgoMIV G'CCGG_C
Cuts at: 0 860 1281
Size: 860 421
NspI r_CATG'y
Cuts at: 0 95 285 1281
Size: 95 190 996
Fragments arranged by size:
996 190 95
PflMI CCAn_nnn'nTGG
Cuts at: 0 723 1281
Size: 723 558
PmlI CAC'GTG
Cuts at: 0 480 1281
Size: 480 801
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 650 682 1281
Size: 650 32 599
Fragments arranged by size:
650 599 32
PvuII CAG'CTG
Cuts at: 0 830 1281
Size: 830 451
SfcI C'TryA_G
Cuts at: 0 1005 1281
Size: 1005 276
SfoI GGC'GCC
Cuts at: 0 290 1281
Size: 290 991
SmlI C'TyrA_G
Cuts at: 0 985 1281
Size: 985 296
SphI G_CATG'C
Cuts at: 0 95 1281
Size: 95 1186
StuI AGG'CCT
Cuts at: 0 104 1001 1138 1281
Size: 104 897 137 143
Fragments arranged by size:
897 143 137 104
StyI C'CwwG_G
Cuts at: 0 26 72 83 1281
Size: 26 46 11 1198
Fragments arranged by size:
1198 46 26 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 649 1189 1281
Size: 253 396 540 92
Fragments arranged by size:
540 396 253 92
TatI w'GTAC_w
Cuts at: 0 107 1281
Size: 107 1174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1105 1137 1234 1266 1281
Size: 1105 32 97 32 15
Fragments arranged by size:
1105 97 32 32 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 910 1281
Size: 910 371
Enzymes that do cut:
AarI AleI AlwNI ApaLI AvaI BanI BanII BbeI
BglI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI Eco57MI
EcoNI EcoO109I HaeII HindIII KasI MmeI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PflMI PmlI PpiI
PvuII SfcI SfoI SmlI SphI StuI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmtI Bpu10I BsaI BsaBI
BsaWI BsiWI BsmI BspEI BspHI BsrBI BsrDI BssHII
BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HpaI KpnI MfeI MluI MscI NdeI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI