Restriction map for
pF1KB6673
(Linear) (Six-base) MAPSORT of: KIBB6673.seq Check: 3749 from: 1 to: 1281
KIBB6673.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 277 1281
Size: 277 1004
Acc65I G'GTAC_C
Cuts at: 0 455 1281
Size: 455 826
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 1281
Size: 378 903
AfeI AGC'GCT
Cuts at: 0 250 1281
Size: 250 1031
AflIII A'CryG_T
Cuts at: 0 512 1281
Size: 512 769
AlwNI CAG_nnn'CTG
Cuts at: 0 84 270 1281
Size: 84 186 1011
Fragments arranged by size:
1011 186 84
ApaI G_GGCC'C
Cuts at: 0 549 1281
Size: 549 732
AvaI C'yCGr_G
Cuts at: 0 314 356 604 1087 1281
Size: 314 42 248 483 194
Fragments arranged by size:
483 314 248 194 42
BanI G'GyrC_C
Cuts at: 0 321 455 570 1184 1213 1281
Size: 321 134 115 614 29 68
Fragments arranged by size:
614 321 134 115 68 29
BanII G_rGCy'C
Cuts at: 0 549 563 840 1281
Size: 549 14 277 441
Fragments arranged by size:
549 441 277 14
BbeI G_GCGC'C
Cuts at: 0 1217 1281
Size: 1217 64
BglI GCCn_nnn'nGGC
Cuts at: 0 319 1124 1281
Size: 319 805 157
Fragments arranged by size:
805 319 157
BlpI GC'TnA_GC
Cuts at: 0 263 1222 1281
Size: 263 959 59
Fragments arranged by size:
959 263 59
Bme1580I G_kGCm'C
Cuts at: 0 549 575 1281
Size: 549 26 706
Fragments arranged by size:
706 549 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 507 532 1281
Size: 507 25 749
Fragments arranged by size:
749 507 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 727 967 1281
Size: 523 204 240 314
Fragments arranged by size:
523 314 240 204
Bpu10I CC'TnA_GC
Cuts at: 0 1114 1281
Size: 1114 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1281
Size: 169 1112
BsaI GGTCTCn'nnnn_
Cuts at: 0 16 1281
Size: 16 1265
BsaAI yAC'GTr
Cuts at: 0 149 681 843 1176 1281
Size: 149 532 162 333 105
Fragments arranged by size:
532 333 162 149 105
BsaHI Gr'CG_yC
Cuts at: 0 1214 1281
Size: 1214 67
BsaWI w'CCGG_w
Cuts at: 0 877 1281
Size: 877 404
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 499 529 700 730 1281
Size: 499 30 171 30 551
Fragments arranged by size:
551 499 171 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 634 1216 1281
Size: 634 582 65
Fragments arranged by size:
634 582 65
BseYI C'CCAG_C
Cuts at: 0 15 533 645 941 1209 1281
Size: 15 518 112 296 268 72
Fragments arranged by size:
518 296 268 112 72 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 523 703 1281
Size: 400 123 180 578
Fragments arranged by size:
578 400 180 123
BsiEI CG_ry'CG
Cuts at: 0 366 1281
Size: 366 915
BsiHKAI G_wGCw'C
Cuts at: 0 116 134 1281
Size: 116 18 1147
Fragments arranged by size:
1147 116 18
BsmI GAATG_Cn'
Cuts at: 0 809 1281
Size: 809 472
Bsp1286I G_dGCh'C
Cuts at: 0 116 134 549 563 575 840 1281
Size: 116 18 415 14 12 265 441
Fragments arranged by size:
441 415 265 116 18 14 12
BspEI T'CCGG_A
Cuts at: 0 877 1281
Size: 877 404
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 277 1281
Size: 201 76 1004
Fragments arranged by size:
1004 201 76
BsrDI GCAATG_nn'
Cuts at: 0 804 1281
Size: 804 477
BsrFI r'CCGG_y
Cuts at: 0 527 784 1281
Size: 527 257 497
Fragments arranged by size:
527 497 257
BstAPI GCAn_nnn'nTGC
Cuts at: 0 681 1281
Size: 681 600
BstXI CCAn_nnnn'nTGG
Cuts at: 0 945 1281
Size: 945 336
BtgI C'CryG_G
Cuts at: 0 413 563 894 1180 1281
Size: 413 150 331 286 101
Fragments arranged by size:
413 331 286 150 101
DraIII CAC_nnn'GTG
Cuts at: 0 501 979 1281
Size: 501 478 302
Fragments arranged by size:
501 478 302
EaeI y'GGCC_r
Cuts at: 0 156 363 416 970 1281
Size: 156 207 53 554 311
Fragments arranged by size:
554 311 207 156 53
EagI C'GGCC_G
Cuts at: 0 363 1281
Size: 363 918
EarI CTCTTCn'nnn_
Cuts at: 0 1048 1281
Size: 1048 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 1060 1281
Size: 244 816 221
Fragments arranged by size:
816 244 221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 523 727 967 1281
Size: 378 145 204 240 314
Fragments arranged by size:
378 314 240 204 145
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1160 1281
Size: 1160 121
EcoO109I rG'GnC_Cy
Cuts at: 0 545 546 885 1023 1153 1281
Size: 545 1 339 138 130 128
Fragments arranged by size:
545 339 138 130 128 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 360 1281
Size: 360 921
FseI GG_CCGG'CC
Cuts at: 0 531 1281
Size: 531 750
HaeII r_GCGC'y
Cuts at: 0 252 1217 1281
Size: 252 965 64
Fragments arranged by size:
965 252 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 210 242 468 499 500 531 700
Size: 26 184 32 226 31 1 31 169
Cuts at: 700 732 1281
Size: 32 549
Fragments arranged by size:
549 226 184 169 32 32 31 31
26 1
KasI G'GCGC_C
Cuts at: 0 1213 1281
Size: 1213 68
KpnI G_GTAC'C
Cuts at: 0 459 1281
Size: 459 822
MluI A'CGCG_T
Cuts at: 0 512 1281
Size: 512 769
MscI TGG'CCA
Cuts at: 0 418 972 1281
Size: 418 554 309
Fragments arranged by size:
554 418 309
MslI CAynn'nnrTG
Cuts at: 0 418 568 1281
Size: 418 150 713
Fragments arranged by size:
713 418 150
MspA1I CmG'CkG
Cuts at: 0 945 1182 1281
Size: 945 237 99
Fragments arranged by size:
945 237 99
NaeI GCC'GGC
Cuts at: 0 529 786 1281
Size: 529 257 495
Fragments arranged by size:
529 495 257
NarI GG'CG_CC
Cuts at: 0 1214 1281
Size: 1214 67
NcoI C'CATG_G
Cuts at: 0 413 563 1281
Size: 413 150 718
Fragments arranged by size:
718 413 150
NgoMIV G'CCGG_C
Cuts at: 0 527 784 1281
Size: 527 257 497
Fragments arranged by size:
527 497 257
NruI TCG'CGA
Cuts at: 0 1082 1281
Size: 1082 199
PflMI CCAn_nnn'nTGG
Cuts at: 0 777 1281
Size: 777 504
PfoI T'CCnGG_A
Cuts at: 0 914 1281
Size: 914 367
PmlI CAC'GTG
Cuts at: 0 149 681 843 1176 1281
Size: 149 532 162 333 105
Fragments arranged by size:
532 333 162 149 105
PpuMI rG'GwC_Cy
Cuts at: 0 885 1023 1153 1281
Size: 885 138 130 128
Fragments arranged by size:
885 138 130 128
PspOMI G'GGCC_C
Cuts at: 0 545 1281
Size: 545 736
PstI C_TGCA'G
Cuts at: 0 56 203 677 1281
Size: 56 147 474 604
Fragments arranged by size:
604 474 147 56
PvuII CAG'CTG
Cuts at: 0 945 1281
Size: 945 336
SacII CC_GC'GG
Cuts at: 0 1183 1281
Size: 1183 98
SanDI GG'GwC_CC
Cuts at: 0 885 1023 1281
Size: 885 138 258
Fragments arranged by size:
885 258 138
SexAI A'CCwGG_T
Cuts at: 0 590 1281
Size: 590 691
SfcI C'TryA_G
Cuts at: 0 52 199 673 1281
Size: 52 147 474 608
Fragments arranged by size:
608 474 147 52
SfoI GGC'GCC
Cuts at: 0 1215 1281
Size: 1215 66
SmaI CCC'GGG
Cuts at: 0 316 1281
Size: 316 965
SmlI C'TyrA_G
Cuts at: 0 184 1281
Size: 184 1097
StuI AGG'CCT
Cuts at: 0 58 902 1281
Size: 58 844 379
Fragments arranged by size:
844 379 58
StyI C'CwwG_G
Cuts at: 0 153 413 563 1281
Size: 153 260 150 718
Fragments arranged by size:
718 260 153 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 489 955 1281
Size: 489 466 326
Fragments arranged by size:
489 466 326
TatI w'GTAC_w
Cuts at: 0 1061 1281
Size: 1061 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 686 718 1281
Size: 686 32 563
Fragments arranged by size:
686 563 32
XmaI C'CCGG_G
Cuts at: 0 314 1281
Size: 314 967
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AlwNI ApaI AvaI
BanI BanII BbeI BglI BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrDI BsrFI BstAPI BstXI BtgI DraIII EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I FalI FseI HaeII
Hin4I KasI KpnI MluI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacII SanDI SexAI SfcI SfoI
SmaI SmlI StuI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BplI BsaBI BsiWI BsmBI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EcoICRI EcoRI
EcoRV FspI FspAI HincII HindIII HpaI MfeI MmeI
NdeI NheI NotI NsiI NspI PacI PasI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SapI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XcmI XhoI XmnI ZraI