Restriction map for
pF1KB6671
(Linear) (Six-base) MAPSORT of: KIBB6671.seq Check: 1960 from: 1 to: 1278
KIBB6671.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 0 14 1278
Size: 14 1264
AflIII A'CryG_T
Cuts at: 0 318 749 1278
Size: 318 431 529
Fragments arranged by size:
529 431 318
AleI CACnn'nnGTG
Cuts at: 0 118 1278
Size: 118 1160
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1144 1164 1176 1196 1278
Size: 1144 20 12 20 82
Fragments arranged by size:
1144 82 20 20 12
AlwNI CAG_nnn'CTG
Cuts at: 0 1169 1278
Size: 1169 109
ApaLI G'TGCA_C
Cuts at: 0 844 1278
Size: 844 434
AvaI C'yCGr_G
Cuts at: 0 224 1014 1278
Size: 224 790 264
Fragments arranged by size:
790 264 224
BanI G'GyrC_C
Cuts at: 0 685 883 1278
Size: 685 198 395
Fragments arranged by size:
685 395 198
BbeI G_GCGC'C
Cuts at: 0 887 1278
Size: 887 391
BbsI GAAGACnn'nnnn_
Cuts at: 0 1108 1278
Size: 1108 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 205 224 239 1278
Size: 190 15 19 15 1039
Fragments arranged by size:
1039 190 19 15 15
BglII A'GATC_T
Cuts at: 0 188 1278
Size: 188 1090
Bme1580I G_kGCm'C
Cuts at: 0 690 848 1204 1276 1278
Size: 690 158 356 72 2
Fragments arranged by size:
690 356 158 72 2
BmgBI CAC'GTC
Cuts at: 0 321 1278
Size: 321 957
BmrI ACTGGGnnnn_n'
Cuts at: 0 285 340 1207 1278
Size: 285 55 867 71
Fragments arranged by size:
867 285 71 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 181 1278
Size: 175 6 1097
Fragments arranged by size:
1097 175 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1278
Size: 238 1040
BsaI GGTCTCn'nnnn_
Cuts at: 0 732 801 1278
Size: 732 69 477
Fragments arranged by size:
732 477 69
BsaAI yAC'GTr
Cuts at: 0 219 972 1278
Size: 219 753 306
Fragments arranged by size:
753 306 219
BsaBI GATnn'nnATC
Cuts at: 0 1219 1278
Size: 1219 59
BsaHI Gr'CG_yC
Cuts at: 0 11 884 1278
Size: 11 873 394
Fragments arranged by size:
873 394 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 14 40 44 70 224 229 254 259
Size: 14 26 4 26 154 5 25 5
Cuts at: 259 580 610 1144 1174 1255 1278
Size: 321 30 534 30 81 23
Fragments arranged by size:
534 321 154 81 30 30 26 26
25 23 14 5 5 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 1255 1278
Size: 154 1101 23
Fragments arranged by size:
1101 154 23
BseYI C'CCAG_C
Cuts at: 0 73 82 1278
Size: 73 9 1196
Fragments arranged by size:
1196 73 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 325 812 1278
Size: 199 126 487 466
Fragments arranged by size:
487 466 199 126
BsiHKAI G_wGCw'C
Cuts at: 0 353 461 848 1278
Size: 353 108 387 430
Fragments arranged by size:
430 387 353 108
Bsp1286I G_dGCh'C
Cuts at: 0 353 461 690 848 1204 1276 1278
Size: 353 108 229 158 356 72 2
Fragments arranged by size:
356 353 229 158 108 72 2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 347 783 1278
Size: 347 436 495
Fragments arranged by size:
495 436 347
BsrBI CCG'CTC
Cuts at: 0 5 235 1278
Size: 5 230 1043
Fragments arranged by size:
1043 230 5
BsrDI GCAATG_nn'
Cuts at: 0 1130 1278
Size: 1130 148
BsrFI r'CCGG_y
Cuts at: 0 1042 1278
Size: 1042 236
BssSI C'ACGA_G
Cuts at: 0 493 1278
Size: 493 785
BstAPI GCAn_nnn'nTGC
Cuts at: 0 348 1278
Size: 348 930
BstEII G'GTnAC_C
Cuts at: 0 975 1278
Size: 975 303
BstXI CCAn_nnnn'nTGG
Cuts at: 0 120 1278
Size: 120 1158
BstYI r'GATC_y
Cuts at: 0 188 1220 1278
Size: 188 1032 58
Fragments arranged by size:
1032 188 58
Bsu36I CC'TnA_GG
Cuts at: 0 905 1278
Size: 905 373
BtgI C'CryG_G
Cuts at: 0 134 367 521 1148 1278
Size: 134 233 154 627 130
Fragments arranged by size:
627 233 154 134 130
BtsI GCAGTG_nn'
Cuts at: 0 610 1054 1261 1278
Size: 610 444 207 17
Fragments arranged by size:
610 444 207 17
DraIII CAC_nnn'GTG
Cuts at: 0 348 975 1278
Size: 348 627 303
Fragments arranged by size:
627 348 303
EaeI y'GGCC_r
Cuts at: 0 131 524 629 1278
Size: 131 393 105 649
Fragments arranged by size:
649 393 131 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 35 1278
Size: 35 1243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 181 1278
Size: 175 6 1097
Fragments arranged by size:
1097 175 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 581 1278
Size: 581 697
EcoO109I rG'GnC_Cy
Cuts at: 0 909 936 1278
Size: 909 27 342
Fragments arranged by size:
909 342 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 123 1278
Size: 123 1155
HaeII r_GCGC'y
Cuts at: 0 40 887 1278
Size: 40 847 391
Fragments arranged by size:
847 391 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 224 229 256 261 580 612
Size: 40 32 152 5 27 5 319 32
Cuts at: 612 884 916 1112 1144 1278
Size: 272 32 196 32 134
Fragments arranged by size:
319 272 196 152 134 40 32 32
32 32 27 5 5
KasI G'GCGC_C
Cuts at: 0 883 1278
Size: 883 395
MscI TGG'CCA
Cuts at: 0 631 1278
Size: 631 647
MslI CAynn'nnrTG
Cuts at: 0 118 598 991 1278
Size: 118 480 393 287
Fragments arranged by size:
480 393 287 118
MspA1I CmG'CkG
Cuts at: 0 136 1278
Size: 136 1142
NarI GG'CG_CC
Cuts at: 0 884 1278
Size: 884 394
NcoI C'CATG_G
Cuts at: 0 1148 1278
Size: 1148 130
NspI r_CATG'y
Cuts at: 0 753 996 1119 1278
Size: 753 243 123 159
Fragments arranged by size:
753 243 159 123
PciI A'CATG_T
Cuts at: 0 749 1278
Size: 749 529
PflMI CCAn_nnn'nTGG
Cuts at: 0 627 737 1278
Size: 627 110 541
Fragments arranged by size:
627 541 110
PfoI T'CCnGG_A
Cuts at: 0 296 620 677 767 1278
Size: 296 324 57 90 511
Fragments arranged by size:
511 324 296 90 57
PmlI CAC'GTG
Cuts at: 0 219 972 1278
Size: 219 753 306
Fragments arranged by size:
753 306 219
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 1144 1176 1278
Size: 394 32 718 32 102
Fragments arranged by size:
718 394 102 32 32
PstI C_TGCA'G
Cuts at: 0 557 794 1064 1278
Size: 557 237 270 214
Fragments arranged by size:
557 270 237 214
SacII CC_GC'GG
Cuts at: 0 137 1278
Size: 137 1141
SfcI C'TryA_G
Cuts at: 0 309 553 790 1060 1278
Size: 309 244 237 270 218
Fragments arranged by size:
309 270 244 237 218
SfoI GGC'GCC
Cuts at: 0 885 1278
Size: 885 393
SgrAI Cr'CCGG_yG
Cuts at: 0 1042 1278
Size: 1042 236
SmaI CCC'GGG
Cuts at: 0 1016 1278
Size: 1016 262
SmlI C'TyrA_G
Cuts at: 0 253 1278
Size: 253 1025
SphI G_CATG'C
Cuts at: 0 1119 1278
Size: 1119 159
SrfI GCCC'GGGC
Cuts at: 0 1016 1278
Size: 1016 262
StuI AGG'CCT
Cuts at: 0 781 1278
Size: 781 497
StyI C'CwwG_G
Cuts at: 0 98 665 1148 1278
Size: 98 567 483 130
Fragments arranged by size:
567 483 130 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 359 529 949 1278
Size: 359 170 420 329
Fragments arranged by size:
420 359 329 170
TatI w'GTAC_w
Cuts at: 0 419 534 1278
Size: 419 115 744
Fragments arranged by size:
744 419 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 265 297 513 545 1255 1278
Size: 14 32 219 32 216 32 710 23
Fragments arranged by size:
710 219 216 32 32 32 23 14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 960 1278
Size: 960 318
XmaI C'CCGG_G
Cuts at: 0 1014 1278
Size: 1014 264
XmnI GAAnn'nnTTC
Cuts at: 0 190 1278
Size: 190 1088
Enzymes that do cut:
AccI AflIII AleI AloI AlwNI ApaLI AvaI BanI
BbeI BbsI BcgI BglII Bme1580I BmgBI BmrI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
KasI MscI MslI MspA1I NarI NcoI NspI PciI
PflMI PfoI PmlI PpiI PstI SacII SfcI SfoI
SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbvCI BciVI BclI BfrBI BglI
BlpI BmtI BplI Bpu10I BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrGI BssHII BstBI BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SnaBI
SpeI SspI SwaI Tth111I XbaI XhoI ZraI