Restriction map for
pF1KB6659
(Linear) (Six-base) MAPSORT of: KIBB6659.seq Check: 3982 from: 1 to: 1272
KIBB6659.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 490 848 1265 1272
Size: 447 43 358 417 7
Fragments arranged by size:
447 417 358 43 7
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 833 865 1272
Size: 833 32 407
Fragments arranged by size:
833 407 32
AlwNI CAG_nnn'CTG
Cuts at: 0 990 1272
Size: 990 282
AvaI C'yCGr_G
Cuts at: 0 85 330 1272
Size: 85 245 942
Fragments arranged by size:
942 245 85
BanII G_rGCy'C
Cuts at: 0 40 1000 1272
Size: 40 960 272
Fragments arranged by size:
960 272 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 1136 1272
Size: 1136 136
BbvCI CC'TCA_GC
Cuts at: 0 455 1272
Size: 455 817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 334 368 397 431 1272
Size: 334 34 29 34 841
Fragments arranged by size:
841 334 34 34 29
BglI GCCn_nnn'nGGC
Cuts at: 0 334 1272
Size: 334 938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 137 292 674 883 967 1024 1272
Size: 129 8 155 382 209 84 57 248
Fragments arranged by size:
382 248 209 155 129 84 57 8
Bpu10I CC'TnA_GC
Cuts at: 0 34 455 1272
Size: 34 421 817
Fragments arranged by size:
817 421 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1272
Size: 907 365
BsaI GGTCTCn'nnnn_
Cuts at: 0 940 1272
Size: 940 332
BsaHI Gr'CG_yC
Cuts at: 0 707 1272
Size: 707 565
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1118 1148 1272
Size: 1118 30 124
Fragments arranged by size:
1118 124 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 598 958 1272
Size: 30 568 360 314
Fragments arranged by size:
568 360 314 30
BseYI C'CCAG_C
Cuts at: 0 97 1272
Size: 97 1175
BsiHKAI G_wGCw'C
Cuts at: 0 40 935 1000 1272
Size: 40 895 65 272
Fragments arranged by size:
895 272 65 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 861 1272
Size: 861 411
Bsp1286I G_dGCh'C
Cuts at: 0 40 935 1000 1272
Size: 40 895 65 272
Fragments arranged by size:
895 272 65 40
BspHI T'CATG_A
Cuts at: 0 713 1272
Size: 713 559
BsrDI GCAATG_nn'
Cuts at: 0 210 1272
Size: 210 1062
BtsI GCAGTG_nn'
Cuts at: 0 316 1272
Size: 316 956
DraIII CAC_nnn'GTG
Cuts at: 0 156 1272
Size: 156 1116
EaeI y'GGCC_r
Cuts at: 0 215 1272
Size: 215 1057
EarI CTCTTCn'nnn_
Cuts at: 0 368 1163 1272
Size: 368 795 109
Fragments arranged by size:
795 368 109
EcoICRI GAG'CTC
Cuts at: 0 38 998 1272
Size: 38 960 274
Fragments arranged by size:
960 274 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 137 292 447 490 674 848 883
Size: 129 8 155 155 43 184 174 35
Cuts at: 883 967 1024 1265 1272
Size: 84 57 241 7
Fragments arranged by size:
241 184 174 155 155 129 84 57
43 35 8 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 529 613 763 1272
Size: 256 273 84 150 509
Fragments arranged by size:
509 273 256 150 84
EcoO109I rG'GnC_Cy
Cuts at: 0 51 189 1272
Size: 51 138 1083
Fragments arranged by size:
1083 138 51
HaeII r_GCGC'y
Cuts at: 0 84 1272
Size: 84 1188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 324 356 1272
Size: 324 32 916
Fragments arranged by size:
916 324 32
HindIII A'AGCT_T
Cuts at: 0 18 1272
Size: 18 1254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 305 1272
Size: 164 141 967
Fragments arranged by size:
967 164 141
MscI TGG'CCA
Cuts at: 0 217 1272
Size: 217 1055
NruI TCG'CGA
Cuts at: 0 344 1272
Size: 344 928
NspI r_CATG'y
Cuts at: 0 78 1272
Size: 78 1194
PpuMI rG'GwC_Cy
Cuts at: 0 189 1272
Size: 189 1083
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1039 1071 1272
Size: 1039 32 201
Fragments arranged by size:
1039 201 32
PstI C_TGCA'G
Cuts at: 0 798 806 1272
Size: 798 8 466
Fragments arranged by size:
798 466 8
SacI G_AGCT'C
Cuts at: 0 40 1000 1272
Size: 40 960 272
Fragments arranged by size:
960 272 40
SapI GCTCTTCn'nnn_
Cuts at: 0 368 1272
Size: 368 904
SfcI C'TryA_G
Cuts at: 0 56 348 794 802 1142 1272
Size: 56 292 446 8 340 130
Fragments arranged by size:
446 340 292 130 56 8
SmlI C'TyrA_G
Cuts at: 0 886 1272
Size: 886 386
SphI G_CATG'C
Cuts at: 0 78 1272
Size: 78 1194
StuI AGG'CCT
Cuts at: 0 524 695 1272
Size: 524 171 577
Fragments arranged by size:
577 524 171
StyI C'CwwG_G
Cuts at: 0 519 702 950 1272
Size: 519 183 248 322
Fragments arranged by size:
519 322 248 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 111 1272
Size: 111 1161
TatI w'GTAC_w
Cuts at: 0 64 1272
Size: 64 1208
XbaI T'CTAG_A
Cuts at: 0 939 1272
Size: 939 333
Enzymes that do cut:
AcuI AloI AlwNI AvaI BanII BbsI BbvCI BcgI
BglI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspHI BsrDI BtsI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HindIII MmeI MscI NruI NspI PpuMI PsrI PstI
SacI SapI SfcI SmlI SphI StuI StyI TaqII
TatI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI