Restriction map for
pF1KB6643
(Linear) (Six-base) MAPSORT of: KIBB6643.seq Check: 1236 from: 1 to: 1161
KIBB6643.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 0 176 1161
Size: 176 985
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 1161
Size: 299 862
AhdI GACnn_n'nnGTC
Cuts at: 0 542 1161
Size: 542 619
ApaI G_GGCC'C
Cuts at: 0 778 1161
Size: 778 383
ApoI r'AATT_y
Cuts at: 0 882 1161
Size: 882 279
AvaI C'yCGr_G
Cuts at: 0 21 1161
Size: 21 1140
BamHI G'GATC_C
Cuts at: 0 472 1161
Size: 472 689
BanII G_rGCy'C
Cuts at: 0 778 1161
Size: 778 383
BbsI GAAGACnn'nnnn_
Cuts at: 0 809 1161
Size: 809 352
BciVI GTATCCnnnnn_n'
Cuts at: 0 1043 1161
Size: 1043 118
BfrBI ATG'CAT
Cuts at: 0 618 910 1161
Size: 618 292 251
Fragments arranged by size:
618 292 251
Bme1580I G_kGCm'C
Cuts at: 0 778 1161
Size: 778 383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 299 1161
Size: 299 862
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 830 1161
Size: 281 549 331
Fragments arranged by size:
549 331 281
BsaBI GATnn'nnATC
Cuts at: 0 200 1161
Size: 200 961
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 755 1161
Size: 755 406
BseYI C'CCAG_C
Cuts at: 0 947 1109 1161
Size: 947 162 52
Fragments arranged by size:
947 162 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1161
Size: 329 832
Bsp1286I G_dGCh'C
Cuts at: 0 778 1161
Size: 778 383
BsrBI CCG'CTC
Cuts at: 0 278 1161
Size: 278 883
BstAPI GCAn_nnn'nTGC
Cuts at: 0 159 1161
Size: 159 1002
BstEII G'GTnAC_C
Cuts at: 0 976 1161
Size: 976 185
BstXI CCAn_nnnn'nTGG
Cuts at: 0 382 1161
Size: 382 779
BstYI r'GATC_y
Cuts at: 0 472 1161
Size: 472 689
BstZ17I GTA'TAC
Cuts at: 0 177 1161
Size: 177 984
BtgI C'CryG_G
Cuts at: 0 468 476 1161
Size: 468 8 685
Fragments arranged by size:
685 468 8
DraIII CAC_nnn'GTG
Cuts at: 0 1015 1161
Size: 1015 146
EaeI y'GGCC_r
Cuts at: 0 372 894 1161
Size: 372 522 267
Fragments arranged by size:
522 372 267
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 677 1161
Size: 677 484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 299 830 1161
Size: 281 18 531 331
Fragments arranged by size:
531 331 281 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 848 1161
Size: 848 313
EcoO109I rG'GnC_Cy
Cuts at: 0 256 337 409 774 841 1024 1161
Size: 256 81 72 365 67 183 137
Fragments arranged by size:
365 256 183 137 81 72 67
EcoRV GAT'ATC
Cuts at: 0 940 1161
Size: 940 221
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 528 560 1161
Size: 528 32 601
Fragments arranged by size:
601 528 32
HincII GTy'rAC
Cuts at: 0 714 1161
Size: 714 447
HpaI GTT'AAC
Cuts at: 0 714 1161
Size: 714 447
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1161
Size: 53 1108
MscI TGG'CCA
Cuts at: 0 896 1161
Size: 896 265
MslI CAynn'nnrTG
Cuts at: 0 451 1161
Size: 451 710
MspA1I CmG'CkG
Cuts at: 0 55 1161
Size: 55 1106
NcoI C'CATG_G
Cuts at: 0 468 476 1161
Size: 468 8 685
Fragments arranged by size:
685 468 8
NdeI CA'TA_TG
Cuts at: 0 395 728 1161
Size: 395 333 433
Fragments arranged by size:
433 395 333
NsiI A_TGCA'T
Cuts at: 0 620 912 1161
Size: 620 292 249
Fragments arranged by size:
620 292 249
NspI r_CATG'y
Cuts at: 0 312 357 519 1161
Size: 312 45 162 642
Fragments arranged by size:
642 312 162 45
PasI CC'CwG_GG
Cuts at: 0 964 1161
Size: 964 197
PfoI T'CCnGG_A
Cuts at: 0 258 1161
Size: 258 903
PpuMI rG'GwC_Cy
Cuts at: 0 256 337 409 841 1024 1161
Size: 256 81 72 432 183 137
Fragments arranged by size:
432 256 183 137 81 72
PspOMI G'GGCC_C
Cuts at: 0 774 1161
Size: 774 387
ScaI AGT'ACT
Cuts at: 0 790 1161
Size: 790 371
SfcI C'TryA_G
Cuts at: 0 756 1161
Size: 756 405
SphI G_CATG'C
Cuts at: 0 519 1161
Size: 519 642
SspI AAT'ATT
Cuts at: 0 868 1161
Size: 868 293
StuI AGG'CCT
Cuts at: 0 1119 1161
Size: 1119 42
StyI C'CwwG_G
Cuts at: 0 468 476 603 675 717 1161
Size: 468 8 127 72 42 444
Fragments arranged by size:
468 444 127 72 42 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 249 1161
Size: 249 912
TatI w'GTAC_w
Cuts at: 0 522 788 1161
Size: 522 266 373
Fragments arranged by size:
522 373 266
Tth111I GACn'n_nGTC
Cuts at: 0 839 1055 1161
Size: 839 216 106
Fragments arranged by size:
839 216 106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 826 1141 1161
Size: 826 315 20
Fragments arranged by size:
826 315 20
Enzymes that do cut:
AccI AcuI AhdI ApaI ApoI AvaI BamHI BanII
BbsI BciVI BfrBI Bme1580I BplI BpmI BsaBI BseRI
BseYI BsgI Bsp1286I BsrBI BstAPI BstEII BstXI BstYI
BstZ17I BtgI DraIII EaeI EciI Eco57MI EcoNI EcoO109I
EcoRV Hin4I HincII HpaI MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PasI PfoI PpuMI PspOMI
ScaI SfcI SphI SspI StuI StyI TaqII TatI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BcgI BclI BglI
BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstBI Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EagI EarI EcoICRI EcoRI FalI FseI FspI
FspAI HaeII HindIII KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SwaI TstI XbaI XhoI XmaI
XmnI ZraI