Restriction map for
pF1KB6636
(Linear) (Six-base) MAPSORT of: KIBB6636.seq Check: 1544 from: 1 to: 1323
KIBB6636.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 464 556 653 1022 1323
Size: 385 79 92 97 369 301
Fragments arranged by size:
385 369 301 97 92 79
AleI CACnn'nnGTG
Cuts at: 0 735 737 1323
Size: 735 2 586
Fragments arranged by size:
735 586 2
AlwNI CAG_nnn'CTG
Cuts at: 0 112 1323
Size: 112 1211
ApaLI G'TGCA_C
Cuts at: 0 1271 1323
Size: 1271 52
ApoI r'AATT_y
Cuts at: 0 410 981 1061 1323
Size: 410 571 80 262
Fragments arranged by size:
571 410 262 80
BamHI G'GATC_C
Cuts at: 0 369 1323
Size: 369 954
BbsI GAAGACnn'nnnn_
Cuts at: 0 605 659 1027 1323
Size: 605 54 368 296
Fragments arranged by size:
605 368 296 54
BbvCI CC'TCA_GC
Cuts at: 0 628 1323
Size: 628 695
BciVI GTATCCnnnnn_n'
Cuts at: 0 921 1323
Size: 921 402
BlpI GC'TnA_GC
Cuts at: 0 758 1323
Size: 758 565
Bme1580I G_kGCm'C
Cuts at: 0 1275 1323
Size: 1275 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 395 442 1323
Size: 134 261 47 881
Fragments arranged by size:
881 261 134 47
Bpu10I CC'TnA_GC
Cuts at: 0 628 1323
Size: 628 695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1323
Size: 827 496
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 60 90 368 398 1323
Size: 60 30 278 30 925
Fragments arranged by size:
925 278 60 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 757 1323
Size: 757 566
BsiHKAI G_wGCw'C
Cuts at: 0 1275 1323
Size: 1275 48
Bsp1286I G_dGCh'C
Cuts at: 0 1275 1323
Size: 1275 48
BsrDI GCAATG_nn'
Cuts at: 0 944 1323
Size: 944 379
BstYI r'GATC_y
Cuts at: 0 369 1323
Size: 369 954
BtsI GCAGTG_nn'
Cuts at: 0 1169 1323
Size: 1169 154
DraI TTT'AAA
Cuts at: 0 956 1323
Size: 956 367
EarI CTCTTCn'nnn_
Cuts at: 0 171 192 1121 1151 1323
Size: 171 21 929 30 172
Fragments arranged by size:
929 172 171 30 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1219 1323
Size: 1219 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 385 395 442 464 556 653 1022
Size: 134 251 10 47 22 92 97 369
Cuts at: 1022 1323
Size: 301
Fragments arranged by size:
369 301 251 134 97 92 47 22
10
EcoRV GAT'ATC
Cuts at: 0 297 1323
Size: 297 1026
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 1323
Size: 463 32 828
Fragments arranged by size:
828 463 32
HincII GTy'rAC
Cuts at: 0 1314 1323
Size: 1314 9
HindIII A'AGCT_T
Cuts at: 0 824 922 1323
Size: 824 98 401
Fragments arranged by size:
824 401 98
MfeI C'AATT_G
Cuts at: 0 344 716 1323
Size: 344 372 607
Fragments arranged by size:
607 372 344
MslI CAynn'nnrTG
Cuts at: 0 637 735 737 1323
Size: 637 98 2 586
Fragments arranged by size:
637 586 98 2
MspA1I CmG'CkG
Cuts at: 0 916 1323
Size: 916 407
NspI r_CATG'y
Cuts at: 0 226 1000 1033 1323
Size: 226 774 33 290
Fragments arranged by size:
774 290 226 33
PfoI T'CCnGG_A
Cuts at: 0 372 1323
Size: 372 951
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1247 1279 1323
Size: 1247 32 44
Fragments arranged by size:
1247 44 32
PstI C_TGCA'G
Cuts at: 0 108 665 1323
Size: 108 557 658
Fragments arranged by size:
658 557 108
SapI GCTCTTCn'nnn_
Cuts at: 0 1121 1323
Size: 1121 202
SfcI C'TryA_G
Cuts at: 0 104 661 1323
Size: 104 557 662
Fragments arranged by size:
662 557 104
SmlI C'TyrA_G
Cuts at: 0 842 1323
Size: 842 481
SphI G_CATG'C
Cuts at: 0 1000 1323
Size: 1000 323
SspI AAT'ATT
Cuts at: 0 860 1323
Size: 860 463
StyI C'CwwG_G
Cuts at: 0 88 1323
Size: 88 1235
TatI w'GTAC_w
Cuts at: 0 147 704 1323
Size: 147 557 619
Fragments arranged by size:
619 557 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 154 186 1323
Size: 154 32 1137
Fragments arranged by size:
1137 154 32
Enzymes that do cut:
AcuI AleI AlwNI ApaLI ApoI BamHI BbsI BbvCI
BciVI BlpI Bme1580I BpmI Bpu10I BpuEI BsaXI BseRI
BsiHKAI Bsp1286I BsrDI BstYI BtsI DraI EarI EciI
Eco57MI EcoRV Hin4I HincII HindIII MfeI MslI MspA1I
NspI PfoI PpiI PstI SapI SfcI SmlI SphI
SspI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BcgI BclI
BfrBI BglI BglII BmgBI BmrI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraIII DrdI EaeI EagI
EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI