Restriction map for
pF1KB6630
(Linear) (Six-base) MAPSORT of: KIBB6630.seq Check: 1902 from: 1 to: 1320
KIBB6630.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1309 1320
Size: 1309 11
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1239 1320
Size: 439 800 81
Fragments arranged by size:
800 439 81
AleI CACnn'nnGTG
Cuts at: 0 1223 1320
Size: 1223 97
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 373 405 1320
Size: 373 32 915
Fragments arranged by size:
915 373 32
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1143 1320
Size: 135 1008 177
Fragments arranged by size:
1008 177 135
AseI AT'TA_AT
Cuts at: 0 923 1320
Size: 923 397
AvaI C'yCGr_G
Cuts at: 0 267 1320
Size: 267 1053
BanI G'GyrC_C
Cuts at: 0 1217 1297 1320
Size: 1217 80 23
Fragments arranged by size:
1217 80 23
BanII G_rGCy'C
Cuts at: 0 81 1320
Size: 81 1239
BbsI GAAGACnn'nnnn_
Cuts at: 0 1062 1320
Size: 1062 258
BglI GCCn_nnn'nGGC
Cuts at: 0 634 1320
Size: 634 686
Bme1580I G_kGCm'C
Cuts at: 0 175 567 1300 1320
Size: 175 392 733 20
Fragments arranged by size:
733 392 175 20
BmgBI CAC'GTC
Cuts at: 0 99 1320
Size: 99 1221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 132 748 779 1210 1320
Size: 95 37 616 31 431 110
Fragments arranged by size:
616 431 110 95 37 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 158 1320
Size: 158 1162
BsaHI Gr'CG_yC
Cuts at: 0 587 1320
Size: 587 733
BsaWI w'CCGG_w
Cuts at: 0 278 500 1320
Size: 278 222 820
Fragments arranged by size:
820 278 222
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 1320
Size: 439 30 851
Fragments arranged by size:
851 439 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 444 591 718 1320
Size: 444 147 127 602
Fragments arranged by size:
602 444 147 127
BseYI C'CCAG_C
Cuts at: 0 854 1320
Size: 854 466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 498 1204 1222 1320
Size: 303 195 706 18 98
Fragments arranged by size:
706 303 195 98 18
BsiHKAI G_wGCw'C
Cuts at: 0 596 1320
Size: 596 724
BsmI GAATG_Cn'
Cuts at: 0 268 778 1320
Size: 268 510 542
Fragments arranged by size:
542 510 268
Bsp1286I G_dGCh'C
Cuts at: 0 81 175 567 596 1300 1320
Size: 81 94 392 29 704 20
Fragments arranged by size:
704 392 94 81 29 20
BspEI T'CCGG_A
Cuts at: 0 278 500 1320
Size: 278 222 820
Fragments arranged by size:
820 278 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 422 1309 1320
Size: 422 887 11
Fragments arranged by size:
887 422 11
BsrDI GCAATG_nn'
Cuts at: 0 1219 1320
Size: 1219 101
BsrFI r'CCGG_y
Cuts at: 0 42 1320
Size: 42 1278
BsrGI T'GTAC_A
Cuts at: 0 434 1320
Size: 434 886
BstAPI GCAn_nnn'nTGC
Cuts at: 0 186 1320
Size: 186 1134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1281 1320
Size: 1281 39
BstYI r'GATC_y
Cuts at: 0 1100 1320
Size: 1100 220
BtgI C'CryG_G
Cuts at: 0 440 1244 1320
Size: 440 804 76
Fragments arranged by size:
804 440 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 638 1320
Size: 638 682
BtsI GCAGTG_nn'
Cuts at: 0 510 1234 1320
Size: 510 724 86
Fragments arranged by size:
724 510 86
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 94 129 1320
Size: 94 35 1191
Fragments arranged by size:
1191 94 35
DraIII CAC_nnn'GTG
Cuts at: 0 1245 1320
Size: 1245 75
EaeI y'GGCC_r
Cuts at: 0 680 1019 1151 1320
Size: 680 339 132 169
Fragments arranged by size:
680 339 169 132
EarI CTCTTCn'nnn_
Cuts at: 0 113 237 393 601 1320
Size: 113 124 156 208 719
Fragments arranged by size:
719 208 156 124 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 132 439 748 779 1210 1239 1320
Size: 95 37 307 309 31 431 29 81
Fragments arranged by size:
431 309 307 95 81 37 31 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 348 1320
Size: 348 972
HaeII r_GCGC'y
Cuts at: 0 1017 1320
Size: 1017 303
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 1320
Size: 319 32 969
Fragments arranged by size:
969 319 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 596 1320
Size: 416 180 724
Fragments arranged by size:
724 416 180
MscI TGG'CCA
Cuts at: 0 682 1021 1320
Size: 682 339 299
Fragments arranged by size:
682 339 299
MslI CAynn'nnrTG
Cuts at: 0 532 783 1223 1320
Size: 532 251 440 97
Fragments arranged by size:
532 440 251 97
MspA1I CmG'CkG
Cuts at: 0 24 1107 1188 1320
Size: 24 1083 81 132
Fragments arranged by size:
1083 132 81 24
NspI r_CATG'y
Cuts at: 0 537 1320
Size: 537 783
PflMI CCAn_nnn'nTGG
Cuts at: 0 884 1143 1320
Size: 884 259 177
Fragments arranged by size:
884 259 177
PsiI TTA'TAA
Cuts at: 0 1010 1320
Size: 1010 310
PstI C_TGCA'G
Cuts at: 0 29 140 1306 1320
Size: 29 111 1166 14
Fragments arranged by size:
1166 111 29 14
PvuII CAG'CTG
Cuts at: 0 1107 1188 1320
Size: 1107 81 132
Fragments arranged by size:
1107 132 81
SapI GCTCTTCn'nnn_
Cuts at: 0 113 601 1320
Size: 113 488 719
Fragments arranged by size:
719 488 113
SbfI CC_TGCA'GG
Cuts at: 0 140 1306 1320
Size: 140 1166 14
Fragments arranged by size:
1166 140 14
ScaI AGT'ACT
Cuts at: 0 670 1320
Size: 670 650
SfcI C'TryA_G
Cuts at: 0 25 136 447 1302 1320
Size: 25 111 311 855 18
Fragments arranged by size:
855 311 111 25 18
StuI AGG'CCT
Cuts at: 0 354 710 1320
Size: 354 356 610
Fragments arranged by size:
610 356 354
StyI C'CwwG_G
Cuts at: 0 480 1125 1268 1320
Size: 480 645 143 52
Fragments arranged by size:
645 480 143 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 839 1320
Size: 839 481
TatI w'GTAC_w
Cuts at: 0 434 668 1320
Size: 434 234 652
Fragments arranged by size:
652 434 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 879 911 1133 1165 1320
Size: 879 32 222 32 155
Fragments arranged by size:
879 222 155 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 357 834 1320
Size: 357 477 486
Fragments arranged by size:
486 477 357
XmnI GAAnn'nnTTC
Cuts at: 0 300 1320
Size: 300 1020
Enzymes that do cut:
AarI AcuI AleI AloI AlwNI AseI AvaI BanI
BanII BbsI BglI Bme1580I BmgBI BpmI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
BtgI BtgZI BtsI CspCI DraIII EaeI EarI Eco57MI
EcoNI HaeII Hin4I MmeI MscI MslI MspA1I NspI
PflMI PsiI PstI PvuII SapI SbfI ScaI SfcI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI ApaI ApaLI ApoI AscI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsiEI BsiWI BsmBI BspHI BsrBI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I ClaI DraI DrdI EagI
EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI ZraI