Restriction map for
pF1KB6629
(Linear) (Six-base) MAPSORT of: KIBB6629.seq Check: 6000 from: 1 to: 1245
KIBB6629.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
Acc65I G'GTAC_C
Cuts at: 0 23 1245
Size: 23 1222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1210 1245
Size: 667 543 35
Fragments arranged by size:
667 543 35
AflIII A'CryG_T
Cuts at: 0 857 1245
Size: 857 388
AhdI GACnn_n'nnGTC
Cuts at: 0 588 1245
Size: 588 657
AlwNI CAG_nnn'CTG
Cuts at: 0 645 1245
Size: 645 600
ApaI G_GGCC'C
Cuts at: 0 1131 1245
Size: 1131 114
ApaLI G'TGCA_C
Cuts at: 0 1237 1245
Size: 1237 8
ApoI r'AATT_y
Cuts at: 0 295 739 756 880 1245
Size: 295 444 17 124 365
Fragments arranged by size:
444 365 295 124 17
BanI G'GyrC_C
Cuts at: 0 23 886 1245
Size: 23 863 359
Fragments arranged by size:
863 359 23
BanII G_rGCy'C
Cuts at: 0 628 1131 1245
Size: 628 503 114
Fragments arranged by size:
628 503 114
BbsI GAAGACnn'nnnn_
Cuts at: 0 1216 1245
Size: 1216 29
BbvCI CC'TCA_GC
Cuts at: 0 140 1245
Size: 140 1105
BclI T'GATC_A
Cuts at: 0 335 1245
Size: 335 910
BglI GCCn_nnn'nGGC
Cuts at: 0 895 1245
Size: 895 350
Bme1580I G_kGCm'C
Cuts at: 0 1131 1241 1245
Size: 1131 110 4
Fragments arranged by size:
1131 110 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 286 1245
Size: 273 13 959
Fragments arranged by size:
959 273 13
Bpu10I CC'TnA_GC
Cuts at: 0 140 1245
Size: 140 1105
BsaAI yAC'GTr
Cuts at: 0 603 858 1245
Size: 603 255 387
Fragments arranged by size:
603 387 255
BsaHI Gr'CG_yC
Cuts at: 0 908 1245
Size: 908 337
BsaWI w'CCGG_w
Cuts at: 0 818 1245
Size: 818 427
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 86 116 271 301 1245
Size: 86 30 155 30 944
Fragments arranged by size:
944 155 86 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 197 949 1134 1245
Size: 197 752 185 111
Fragments arranged by size:
752 197 185 111
BseYI C'CCAG_C
Cuts at: 0 30 252 1245
Size: 30 222 993
Fragments arranged by size:
993 222 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1102 1245
Size: 1102 143
BsiEI CG_ry'CG
Cuts at: 0 996 1245
Size: 996 249
BsiHKAI G_wGCw'C
Cuts at: 0 1241 1245
Size: 1241 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1083 1245
Size: 1083 162
Bsp1286I G_dGCh'C
Cuts at: 0 628 1131 1241 1245
Size: 628 503 110 4
Fragments arranged by size:
628 503 110 4
BspEI T'CCGG_A
Cuts at: 0 818 1245
Size: 818 427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 812 1245
Size: 812 433
BsrBI CCG'CTC
Cuts at: 0 915 939 1098 1245
Size: 915 24 159 147
Fragments arranged by size:
915 159 147 24
BsrDI GCAATG_nn'
Cuts at: 0 83 1245
Size: 83 1162
BsrFI r'CCGG_y
Cuts at: 0 26 1245
Size: 26 1219
BsrGI T'GTAC_A
Cuts at: 0 434 1245
Size: 434 811
BtgI C'CryG_G
Cuts at: 0 969 1122 1245
Size: 969 153 123
Fragments arranged by size:
969 153 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 817 1245
Size: 817 428
DrdI GACnn_nn'nnGTC
Cuts at: 0 232 1245
Size: 232 1013
EaeI y'GGCC_r
Cuts at: 0 455 993 1245
Size: 455 538 252
Fragments arranged by size:
538 455 252
EagI C'GGCC_G
Cuts at: 0 993 1245
Size: 993 252
EarI CTCTTCn'nnn_
Cuts at: 0 92 280 570 1245
Size: 92 188 290 675
Fragments arranged by size:
675 290 188 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 286 667 1210 1245
Size: 273 13 381 543 35
Fragments arranged by size:
543 381 273 35 13
EcoO109I rG'GnC_Cy
Cuts at: 0 270 1127 1128 1245
Size: 270 857 1 117
Fragments arranged by size:
857 270 117 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 574 606 1188 1220 1245
Size: 176 32 366 32 582 32 25
Fragments arranged by size:
582 366 176 32 32 32 25
HincII GTy'rAC
Cuts at: 0 1137 1245
Size: 1137 108
HindIII A'AGCT_T
Cuts at: 0 112 1245
Size: 112 1133
KpnI G_GTAC'C
Cuts at: 0 27 1245
Size: 27 1218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 1245
Size: 1175 70
MscI TGG'CCA
Cuts at: 0 457 1245
Size: 457 788
MslI CAynn'nnrTG
Cuts at: 0 241 380 712 1245
Size: 241 139 332 533
Fragments arranged by size:
533 332 241 139
MspA1I CmG'CkG
Cuts at: 0 50 971 1124 1245
Size: 50 921 153 121
Fragments arranged by size:
921 153 121 50
NspI r_CATG'y
Cuts at: 0 876 1185 1245
Size: 876 309 60
Fragments arranged by size:
876 309 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 206 1245
Size: 206 1039
PfoI T'CCnGG_A
Cuts at: 0 289 1245
Size: 289 956
PpuMI rG'GwC_Cy
Cuts at: 0 270 1245
Size: 270 975
PspOMI G'GGCC_C
Cuts at: 0 1127 1245
Size: 1127 118
PstI C_TGCA'G
Cuts at: 0 93 1245
Size: 93 1152
SacII CC_GC'GG
Cuts at: 0 972 1125 1245
Size: 972 153 120
Fragments arranged by size:
972 153 120
SexAI A'CCwGG_T
Cuts at: 0 428 1245
Size: 428 817
SfcI C'TryA_G
Cuts at: 0 89 1245
Size: 89 1156
StyI C'CwwG_G
Cuts at: 0 555 1245
Size: 555 690
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 496 944 1245
Size: 496 448 301
Fragments arranged by size:
496 448 301
TatI w'GTAC_w
Cuts at: 0 434 611 1245
Size: 434 177 634
Fragments arranged by size:
634 434 177
XmnI GAAnn'nnTTC
Cuts at: 0 353 826 1245
Size: 353 473 419
Fragments arranged by size:
473 419 353
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AlwNI ApaI ApaLI ApoI
BanI BanII BbsI BbvCI BclI BglI Bme1580I BpmI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BtgI BtgZI DrdI EaeI EagI EarI
Eco57MI EcoO109I Hin4I HincII HindIII KpnI MmeI MscI
MslI MspA1I NspI PflMI PfoI PpuMI PspOMI PstI
SacII SexAI SfcI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BcgI BciVI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI BpuEI BsaI BsaBI BsiWI BsmI
BspHI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI