Restriction map for
pF1KB6628
(Linear) (Six-base) MAPSORT of: KIBB6628.seq Check: 6379 from: 1 to: 1218
KIBB6628.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 976 1218
Size: 976 242
AccI GT'mk_AC
Cuts at: 0 273 891 1218
Size: 273 618 327
Fragments arranged by size:
618 327 273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1218
Size: 743 475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 434 1218
Size: 434 784
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1156 1188 1218
Size: 1156 32 30
Fragments arranged by size:
1156 32 30
AlwNI CAG_nnn'CTG
Cuts at: 0 969 1218
Size: 969 249
ApaI G_GGCC'C
Cuts at: 0 858 1125 1218
Size: 858 267 93
Fragments arranged by size:
858 267 93
ApoI r'AATT_y
Cuts at: 0 425 535 1218
Size: 425 110 683
Fragments arranged by size:
683 425 110
AvaI C'yCGr_G
Cuts at: 0 434 1218
Size: 434 784
BanII G_rGCy'C
Cuts at: 0 752 858 1125 1218
Size: 752 106 267 93
Fragments arranged by size:
752 267 106 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 731 1218
Size: 731 487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 144 178 1218
Size: 144 34 1040
Fragments arranged by size:
1040 144 34
BglI GCCn_nnn'nGGC
Cuts at: 0 853 1081 1218
Size: 853 228 137
Fragments arranged by size:
853 228 137
BglII A'GATC_T
Cuts at: 0 438 1218
Size: 438 780
Bme1580I G_kGCm'C
Cuts at: 0 849 858 1125 1218
Size: 849 9 267 93
Fragments arranged by size:
849 267 93 9
BmgBI CAC'GTC
Cuts at: 0 508 1218
Size: 508 710
BmrI ACTGGGnnnn_n'
Cuts at: 0 359 587 1170 1218
Size: 359 228 583 48
Fragments arranged by size:
583 359 228 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1176 1218
Size: 463 713 42
Fragments arranged by size:
713 463 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 136 1218
Size: 136 1082
BsaAI yAC'GTr
Cuts at: 0 484 531 1218
Size: 484 47 687
Fragments arranged by size:
687 484 47
BsaBI GATnn'nnATC
Cuts at: 0 194 1218
Size: 194 1024
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 201 213 231 243 1156 1186 1218
Size: 201 12 18 12 913 30 32
Fragments arranged by size:
913 201 32 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 1218
Size: 394 824
BseYI C'CCAG_C
Cuts at: 0 263 591 1218
Size: 263 328 627
Fragments arranged by size:
627 328 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 1218
Size: 691 527
Bsp1286I G_dGCh'C
Cuts at: 0 752 849 858 1125 1218
Size: 752 97 9 267 93
Fragments arranged by size:
752 267 97 93 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 668 976 1218
Size: 668 308 242
Fragments arranged by size:
668 308 242
BsrBI CCG'CTC
Cuts at: 0 803 1218
Size: 803 415
BsrDI GCAATG_nn'
Cuts at: 0 144 521 678 1218
Size: 144 377 157 540
Fragments arranged by size:
540 377 157 144
BsrGI T'GTAC_A
Cuts at: 0 609 1218
Size: 609 609
BstAPI GCAn_nnn'nTGC
Cuts at: 0 588 1218
Size: 588 630
BstYI r'GATC_y
Cuts at: 0 438 972 1218
Size: 438 534 246
Fragments arranged by size:
534 438 246
Bsu36I CC'TnA_GG
Cuts at: 0 377 1218
Size: 377 841
BtgI C'CryG_G
Cuts at: 0 647 1218
Size: 647 571
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 491 1218
Size: 491 727
BtsI GCAGTG_nn'
Cuts at: 0 247 1046 1218
Size: 247 799 172
Fragments arranged by size:
799 247 172
EaeI y'GGCC_r
Cuts at: 0 563 650 906 1218
Size: 563 87 256 312
Fragments arranged by size:
563 312 256 87
EarI CTCTTCn'nnn_
Cuts at: 0 56 1218
Size: 56 1162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 743 1176 1218
Size: 463 280 433 42
Fragments arranged by size:
463 433 280 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 664 781 1218
Size: 664 117 437
Fragments arranged by size:
664 437 117
EcoO109I rG'GnC_Cy
Cuts at: 0 855 1121 1122 1218
Size: 855 266 1 96
Fragments arranged by size:
855 266 96 1
EcoRI G'AATT_C
Cuts at: 0 425 535 1218
Size: 425 110 683
Fragments arranged by size:
683 425 110
HaeII r_GCGC'y
Cuts at: 0 1028 1218
Size: 1028 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 162 194 201 213 233 245 439 471
Size: 162 32 7 12 20 12 194 32
Cuts at: 471 487 519 587 619 964 996 1218
Size: 16 32 68 32 345 32 222
Fragments arranged by size:
345 222 194 162 68 32 32 32
32 32 20 16 12 12 7
HincII GTy'rAC
Cuts at: 0 274 892 1218
Size: 274 618 326
Fragments arranged by size:
618 326 274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1059 1218
Size: 199 860 159
Fragments arranged by size:
860 199 159
MscI TGG'CCA
Cuts at: 0 565 652 908 1218
Size: 565 87 256 310
Fragments arranged by size:
565 310 256 87
MslI CAynn'nnrTG
Cuts at: 0 281 308 1218
Size: 281 27 910
Fragments arranged by size:
910 281 27
MspA1I CmG'CkG
Cuts at: 0 585 1218
Size: 585 633
NcoI C'CATG_G
Cuts at: 0 647 1218
Size: 647 571
NruI TCG'CGA
Cuts at: 0 291 476 1218
Size: 291 185 742
Fragments arranged by size:
742 291 185
NspI r_CATG'y
Cuts at: 0 732 1218
Size: 732 486
PflMI CCAn_nnn'nTGG
Cuts at: 0 969 1218
Size: 969 249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1156 1188 1218
Size: 1156 32 30
Fragments arranged by size:
1156 32 30
PshAI GACnn'nnGTC
Cuts at: 0 305 1218
Size: 305 913
PspOMI G'GGCC_C
Cuts at: 0 854 1121 1218
Size: 854 267 97
Fragments arranged by size:
854 267 97
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 455 487 1218
Size: 455 32 731
Fragments arranged by size:
731 455 32
SalI G'TCGA_C
Cuts at: 0 272 890 1218
Size: 272 618 328
Fragments arranged by size:
618 328 272
SfcI C'TryA_G
Cuts at: 0 461 1218
Size: 461 757
SmlI C'TyrA_G
Cuts at: 0 434 1218
Size: 434 784
SnaBI TAC'GTA
Cuts at: 0 484 1218
Size: 484 734
SspI AAT'ATT
Cuts at: 0 771 1218
Size: 771 447
StyI C'CwwG_G
Cuts at: 0 647 1115 1218
Size: 647 468 103
Fragments arranged by size:
647 468 103
TatI w'GTAC_w
Cuts at: 0 609 1218
Size: 609 609
XhoI C'TCGA_G
Cuts at: 0 434 1218
Size: 434 784
Enzymes that do cut:
AarI AccI AcuI AlfI AloI AlwNI ApaI ApoI
AvaI BanII BbsI BcgI BglI BglII Bme1580I BmgBI
BmrI BpmI BsaI BsaAI BsaBI BsaXI BseRI BseYI
BsgI Bsp1286I BspMI BsrBI BsrDI BsrGI BstAPI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NcoI NruI NspI PflMI PpiI PshAI PspOMI
PsrI SalI SfcI SmlI SnaBI SspI StyI TatI
XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BciVI BclI BfrBI BlpI BmtI
BplI Bpu10I BpuEI BsaHI BsaWI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NsiI PacI PasI PciI
PfoI PmeI PmlI PpuMI PsiI PspXI PstI PvuI
PvuII RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI
SrfI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XmaI XmnI ZraI