Restriction map for
pF1KB6626
(Linear) (Six-base) MAPSORT of: KIBB6626.seq Check: 5453 from: 1 to: 1317
KIBB6626.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 944 1317
Size: 944 373
Acc65I G'GTAC_C
Cuts at: 0 317 1317
Size: 317 1000
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 193 343 1317
Size: 88 105 150 974
Fragments arranged by size:
974 150 105 88
AleI CACnn'nnGTG
Cuts at: 0 862 1317
Size: 862 455
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1151 1317
Size: 1151 166
AlwNI CAG_nnn'CTG
Cuts at: 0 1077 1317
Size: 1077 240
AseI AT'TA_AT
Cuts at: 0 1238 1317
Size: 1238 79
AvaI C'yCGr_G
Cuts at: 0 1081 1317
Size: 1081 236
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 352 385 1317
Size: 352 33 932
Fragments arranged by size:
932 352 33
BamHI G'GATC_C
Cuts at: 0 3 614 1317
Size: 3 611 703
Fragments arranged by size:
703 611 3
BanI G'GyrC_C
Cuts at: 0 206 317 1047 1317
Size: 206 111 730 270
Fragments arranged by size:
730 270 206 111
BanII G_rGCy'C
Cuts at: 0 670 773 983 1316 1317
Size: 670 103 210 333 1
Fragments arranged by size:
670 333 210 103 1
BbvCI CC'TCA_GC
Cuts at: 0 409 1317
Size: 409 908
BglI GCCn_nnn'nGGC
Cuts at: 0 699 1317
Size: 699 618
BglII A'GATC_T
Cuts at: 0 554 1317
Size: 554 763
BlpI GC'TnA_GC
Cuts at: 0 583 1317
Size: 583 734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 730 1054 1317
Size: 622 108 324 263
Fragments arranged by size:
622 324 263 108
Bpu10I CC'TnA_GC
Cuts at: 0 409 574 977 1317
Size: 409 165 403 340
Fragments arranged by size:
409 403 340 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1213 1317
Size: 937 276 104
Fragments arranged by size:
937 276 104
BsaAI yAC'GTr
Cuts at: 0 345 594 1317
Size: 345 249 723
Fragments arranged by size:
723 345 249
BsaWI w'CCGG_w
Cuts at: 0 842 1317
Size: 842 475
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 388 418 1317
Size: 388 30 899
Fragments arranged by size:
899 388 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1099 1317
Size: 1099 218
BseYI C'CCAG_C
Cuts at: 0 283 609 1306 1317
Size: 283 326 697 11
Fragments arranged by size:
697 326 283 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 898 916 1121 1147 1317
Size: 358 540 18 205 26 170
Fragments arranged by size:
540 358 205 170 26 18
BsiHKAI G_wGCw'C
Cuts at: 0 593 632 1317
Size: 593 39 685
Fragments arranged by size:
685 593 39
BsmI GAATG_Cn'
Cuts at: 0 281 1317
Size: 281 1036
Bsp1286I G_dGCh'C
Cuts at: 0 593 632 670 773 983 1316 1317
Size: 593 39 38 103 210 333 1
Fragments arranged by size:
593 333 210 103 39 38 1
BspEI T'CCGG_A
Cuts at: 0 842 1317
Size: 842 475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 944 1053 1205 1317
Size: 944 109 152 112
Fragments arranged by size:
944 152 112 109
BsrBI CCG'CTC
Cuts at: 0 9 1317
Size: 9 1308
BsrGI T'GTAC_A
Cuts at: 0 473 1317
Size: 473 844
BstXI CCAn_nnnn'nTGG
Cuts at: 0 724 1317
Size: 724 593
BstYI r'GATC_y
Cuts at: 0 3 554 614 1317
Size: 3 551 60 703
Fragments arranged by size:
703 551 60 3
BtgI C'CryG_G
Cuts at: 0 378 697 1317
Size: 378 319 620
Fragments arranged by size:
620 378 319
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 183 1317
Size: 183 1134
BtsI GCAGTG_nn'
Cuts at: 0 1057 1317
Size: 1057 260
ClaI AT'CG_AT
Cuts at: 0 83 1317
Size: 83 1234
DraIII CAC_nnn'GTG
Cuts at: 0 1144 1317
Size: 1144 173
EaeI y'GGCC_r
Cuts at: 0 851 1217 1317
Size: 851 366 100
Fragments arranged by size:
851 366 100
EarI CTCTTCn'nnn_
Cuts at: 0 523 658 1317
Size: 523 135 659
Fragments arranged by size:
659 523 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 261 1317
Size: 261 1056
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 193 343 622 730 1054 1317
Size: 88 105 150 279 108 324 263
Fragments arranged by size:
324 279 263 150 108 105 88
EcoO109I rG'GnC_Cy
Cuts at: 0 1065 1317
Size: 1065 252
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1317
Size: 27 1290
KpnI G_GTAC'C
Cuts at: 0 321 1317
Size: 321 996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1317
Size: 511 806
MslI CAynn'nnrTG
Cuts at: 0 862 1317
Size: 862 455
MspA1I CmG'CkG
Cuts at: 0 66 413 609 1310 1317
Size: 66 347 196 701 7
Fragments arranged by size:
701 347 196 66 7
PflMI CCAn_nnn'nTGG
Cuts at: 0 216 345 1014 1317
Size: 216 129 669 303
Fragments arranged by size:
669 303 216 129
PfoI T'CCnGG_A
Cuts at: 0 746 869 965 1317
Size: 746 123 96 352
Fragments arranged by size:
746 352 123 96
PmlI CAC'GTG
Cuts at: 0 594 1317
Size: 594 723
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 1317
Size: 85 32 1200
Fragments arranged by size:
1200 85 32
PpuMI rG'GwC_Cy
Cuts at: 0 1065 1317
Size: 1065 252
PstI C_TGCA'G
Cuts at: 0 23 740 1064 1317
Size: 23 717 324 253
Fragments arranged by size:
717 324 253 23
PvuII CAG'CTG
Cuts at: 0 66 413 609 1317
Size: 66 347 196 708
Fragments arranged by size:
708 347 196 66
SapI GCTCTTCn'nnn_
Cuts at: 0 658 1317
Size: 658 659
SexAI A'CCwGG_T
Cuts at: 0 1094 1317
Size: 1094 223
SfcI C'TryA_G
Cuts at: 0 19 736 1060 1317
Size: 19 717 324 257
Fragments arranged by size:
717 324 257 19
SmlI C'TyrA_G
Cuts at: 0 952 1228 1317
Size: 952 276 89
Fragments arranged by size:
952 276 89
StuI AGG'CCT
Cuts at: 0 469 1317
Size: 469 848
StyI C'CwwG_G
Cuts at: 0 35 1208 1278 1317
Size: 35 1173 70 39
Fragments arranged by size:
1173 70 39 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 874 1317
Size: 874 443
TatI w'GTAC_w
Cuts at: 0 449 473 783 887 920 1317
Size: 449 24 310 104 33 397
Fragments arranged by size:
449 397 310 104 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1049 1081 1317
Size: 1049 32 236
Fragments arranged by size:
1049 236 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 861 1170 1266 1317
Size: 861 309 96 51
Fragments arranged by size:
861 309 96 51
XmnI GAAnn'nnTTC
Cuts at: 0 556 1317
Size: 556 761
Enzymes that do cut:
AarI Acc65I AcuI AleI AlfI AlwNI AseI AvaI
BaeI BamHI BanI BanII BbvCI BglI BglII BlpI
BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrGI
BstXI BstYI BtgI BtgZI BtsI ClaI DraIII EaeI
EarI EciI Eco57MI EcoO109I Hin4I KpnI MmeI MslI
MspA1I PflMI PfoI PmlI PpiI PpuMI PstI PvuII
SapI SexAI SfcI SmlI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AsiSI AvrII BbeI
BbsI BcgI BciVI BclI BfrBI Bme1580I BmgBI BmrI
BmtI BplI BsaI BsaBI BsaHI BsiEI BsiWI BsmBI
BspHI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I CspCI DraI DrdI EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
ZraI