Restriction map for
pF1KB6615
(Linear) (Six-base) MAPSORT of: KIBB6615.seq Check: 155 from: 1 to: 1236
KIBB6615.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 405 1236
Size: 405 831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 427 1236
Size: 427 809
AlwNI CAG_nnn'CTG
Cuts at: 0 48 378 1236
Size: 48 330 858
Fragments arranged by size:
858 330 48
ApaI G_GGCC'C
Cuts at: 0 171 1059 1236
Size: 171 888 177
Fragments arranged by size:
888 177 171
AvaI C'yCGr_G
Cuts at: 0 742 871 1236
Size: 742 129 365
Fragments arranged by size:
742 365 129
BanI G'GyrC_C
Cuts at: 0 331 691 1236
Size: 331 360 545
Fragments arranged by size:
545 360 331
BanII G_rGCy'C
Cuts at: 0 62 171 193 1059 1236
Size: 62 109 22 866 177
Fragments arranged by size:
866 177 109 62 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 894 1236
Size: 894 342
BglI GCCn_nnn'nGGC
Cuts at: 0 57 301 875 1054 1063 1236
Size: 57 244 574 179 9 173
Fragments arranged by size:
574 244 179 173 57 9
BlpI GC'TnA_GC
Cuts at: 0 967 1236
Size: 967 269
Bme1580I G_kGCm'C
Cuts at: 0 171 1059 1123 1236
Size: 171 888 64 113
Fragments arranged by size:
888 171 113 64
BmgBI CAC'GTC
Cuts at: 0 624 1236
Size: 624 612
BmrI ACTGGGnnnn_n'
Cuts at: 0 403 1031 1236
Size: 403 628 205
Fragments arranged by size:
628 403 205
BmtI G_CTAG'C
Cuts at: 0 448 631 1236
Size: 448 183 605
Fragments arranged by size:
605 448 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 290 400 1114 1236
Size: 46 244 110 714 122
Fragments arranged by size:
714 244 122 110 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 969 1236
Size: 139 830 267
Fragments arranged by size:
830 267 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 986 1016 1236
Size: 986 30 220
Fragments arranged by size:
986 220 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 786 1236
Size: 786 450
BseYI C'CCAG_C
Cuts at: 0 432 448 678 1101 1116 1222 1236
Size: 432 16 230 423 15 106 14
Fragments arranged by size:
432 423 230 106 16 15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 391 433 533 1236
Size: 61 330 42 100 703
Fragments arranged by size:
703 330 100 61 42
BsiEI CG_ry'CG
Cuts at: 0 389 1236
Size: 389 847
BsiHKAI G_wGCw'C
Cuts at: 0 668 1236
Size: 668 568
Bsp1286I G_dGCh'C
Cuts at: 0 62 171 193 668 1059 1123 1236
Size: 62 109 22 475 391 64 113
Fragments arranged by size:
475 391 113 109 64 62 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 405 1236
Size: 405 831
BsrFI r'CCGG_y
Cuts at: 0 25 94 384 1236
Size: 25 69 290 852
Fragments arranged by size:
852 290 69 25
BssHII G'CGCG_C
Cuts at: 0 455 1236
Size: 455 781
BssSI C'ACGA_G
Cuts at: 0 483 1236
Size: 483 753
BstAPI GCAn_nnn'nTGC
Cuts at: 0 48 615 1236
Size: 48 567 621
Fragments arranged by size:
621 567 48
BstEII G'GTnAC_C
Cuts at: 0 1002 1236
Size: 1002 234
BtgI C'CryG_G
Cuts at: 0 145 530 726 1236
Size: 145 385 196 510
Fragments arranged by size:
510 385 196 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 771 1236
Size: 771 465
EaeI y'GGCC_r
Cuts at: 0 23 125 276 386 487 527 818 1085
Size: 23 102 151 110 101 40 291 267
Cuts at: 1085 1127 1236
Size: 42 109
Fragments arranged by size:
291 267 151 110 109 102 101 42
40 23
EagI C'GGCC_G
Cuts at: 0 386 1236
Size: 386 850
EarI CTCTTCn'nnn_
Cuts at: 0 716 1236
Size: 716 520
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 856 1236
Size: 856 380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 290 400 1114 1236
Size: 46 244 110 714 122
Fragments arranged by size:
714 244 122 110 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 40 1236
Size: 40 1196
EcoO109I rG'GnC_Cy
Cuts at: 0 45 168 200 809 876 1055 1056 1236
Size: 45 123 32 609 67 179 1 180
Fragments arranged by size:
609 180 179 123 67 45 32 1
FseI GG_CCGG'CC
Cuts at: 0 29 1236
Size: 29 1207
HaeII r_GCGC'y
Cuts at: 0 469 1236
Size: 469 767
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 960 986 992 1018 1236
Size: 960 26 6 26 218
Fragments arranged by size:
960 218 26 26 6
MscI TGG'CCA
Cuts at: 0 127 278 820 1087 1236
Size: 127 151 542 267 149
Fragments arranged by size:
542 267 151 149 127
MslI CAynn'nnrTG
Cuts at: 0 1024 1236
Size: 1024 212
MspA1I CmG'CkG
Cuts at: 0 21 174 298 375 532 1044 1236
Size: 21 153 124 77 157 512 192
Fragments arranged by size:
512 192 157 153 124 77 21
NaeI GCC'GGC
Cuts at: 0 27 96 386 1236
Size: 27 69 290 850
Fragments arranged by size:
850 290 69 27
NgoMIV G'CCGG_C
Cuts at: 0 25 94 384 1236
Size: 25 69 290 852
Fragments arranged by size:
852 290 69 25
NheI G'CTAG_C
Cuts at: 0 444 627 1236
Size: 444 183 609
Fragments arranged by size:
609 444 183
PasI CC'CwG_GG
Cuts at: 0 801 918 1052 1236
Size: 801 117 134 184
Fragments arranged by size:
801 184 134 117
PflMI CCAn_nnn'nTGG
Cuts at: 0 521 827 1236
Size: 521 306 409
Fragments arranged by size:
521 409 306
PfoI T'CCnGG_A
Cuts at: 0 267 1236
Size: 267 969
PpuMI rG'GwC_Cy
Cuts at: 0 809 1236
Size: 809 427
PspOMI G'GGCC_C
Cuts at: 0 167 1055 1236
Size: 167 888 181
Fragments arranged by size:
888 181 167
PstI C_TGCA'G
Cuts at: 0 983 1039 1064 1236
Size: 983 56 25 172
Fragments arranged by size:
983 172 56 25
PvuII CAG'CTG
Cuts at: 0 375 1044 1236
Size: 375 669 192
Fragments arranged by size:
669 375 192
SacII CC_GC'GG
Cuts at: 0 533 1236
Size: 533 703
SbfI CC_TGCA'GG
Cuts at: 0 1064 1236
Size: 1064 172
SexAI A'CCwGG_T
Cuts at: 0 35 1236
Size: 35 1201
SfcI C'TryA_G
Cuts at: 0 979 1035 1060 1074 1236
Size: 979 56 25 14 162
Fragments arranged by size:
979 162 56 25 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1054 1063 1236
Size: 1054 9 173
Fragments arranged by size:
1054 173 9
SmlI C'TyrA_G
Cuts at: 0 154 948 1236
Size: 154 794 288
Fragments arranged by size:
794 288 154
StuI AGG'CCT
Cuts at: 0 998 1136 1236
Size: 998 138 100
Fragments arranged by size:
998 138 100
StyI C'CwwG_G
Cuts at: 0 128 896 1236
Size: 128 768 340
Fragments arranged by size:
768 340 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 195 319 670 757 793 1236
Size: 195 124 351 87 36 443
Fragments arranged by size:
443 351 195 124 87 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 522 1236
Size: 345 177 714
Fragments arranged by size:
714 345 177
Enzymes that do cut:
AarI AlfI AlwNI ApaI AvaI BanI BanII BbsI
BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrFI BssHII BssSI BstAPI BstEII BtgI BtgZI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FseI HaeII
Hin4I MscI MslI MspA1I NaeI NgoMIV NheI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacII SbfI
SexAI SfcI SfiI SmlI StuI StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BplI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BstBI BstXI BstYI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV
FalI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NcoI NdeI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI