Restriction map for
pF1KB6608
(Linear) (Six-base) MAPSORT of: KIBB6608.seq Check: 2193 from: 1 to: 1293
KIBB6608.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 396 848 1293
Size: 396 452 445
Fragments arranged by size:
452 445 396
AccI GT'mk_AC
Cuts at: 0 710 1223 1293
Size: 710 513 70
Fragments arranged by size:
710 513 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 319 856 1079 1225 1293
Size: 283 36 537 223 146 68
Fragments arranged by size:
537 283 223 146 68 36
AfeI AGC'GCT
Cuts at: 0 798 1293
Size: 798 495
AflIII A'CryG_T
Cuts at: 0 112 620 1293
Size: 112 508 673
Fragments arranged by size:
673 508 112
AhdI GACnn_n'nnGTC
Cuts at: 0 1138 1293
Size: 1138 155
AleI CACnn'nnGTG
Cuts at: 0 619 1293
Size: 619 674
ApoI r'AATT_y
Cuts at: 0 166 493 547 963 1293
Size: 166 327 54 416 330
Fragments arranged by size:
416 330 327 166 54
AscI GG'CGCG_CC
Cuts at: 0 16 1293
Size: 16 1277
BamHI G'GATC_C
Cuts at: 0 1253 1293
Size: 1253 40
BanI G'GyrC_C
Cuts at: 0 596 1293
Size: 596 697
BanII G_rGCy'C
Cuts at: 0 9 610 1293
Size: 9 601 683
Fragments arranged by size:
683 601 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 377 411 1293
Size: 377 34 882
Fragments arranged by size:
882 377 34
BclI T'GATC_A
Cuts at: 0 656 1293
Size: 656 637
BglI GCCn_nnn'nGGC
Cuts at: 0 13 1293
Size: 13 1280
BglII A'GATC_T
Cuts at: 0 322 1293
Size: 322 971
Bme1580I G_kGCm'C
Cuts at: 0 1203 1293
Size: 1203 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 915 1293
Size: 915 378
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 652 1293
Size: 652 641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1293
Size: 464 829
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 1293
Size: 708 585
BsaAI yAC'GTr
Cuts at: 0 621 1293
Size: 621 672
BsaHI Gr'CG_yC
Cuts at: 0 1220 1293
Size: 1220 73
BsaWI w'CCGG_w
Cuts at: 0 380 984 1293
Size: 380 604 309
Fragments arranged by size:
604 380 309
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 742 772 844 874 1293
Size: 742 30 72 30 419
Fragments arranged by size:
742 419 72 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 462 467 1293
Size: 462 5 826
Fragments arranged by size:
826 462 5
BseYI C'CCAG_C
Cuts at: 0 339 650 1184 1293
Size: 339 311 534 109
Fragments arranged by size:
534 339 311 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 1293
Size: 424 869
Bsp1286I G_dGCh'C
Cuts at: 0 9 610 1203 1293
Size: 9 601 593 90
Fragments arranged by size:
601 593 90 9
BspEI T'CCGG_A
Cuts at: 0 984 1293
Size: 984 309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 214 396 848 1293
Size: 214 182 452 445
Fragments arranged by size:
452 445 214 182
BsrDI GCAATG_nn'
Cuts at: 0 69 824 1293
Size: 69 755 469
Fragments arranged by size:
755 469 69
BsrFI r'CCGG_y
Cuts at: 0 263 1293
Size: 263 1030
BssHII G'CGCG_C
Cuts at: 0 16 1293
Size: 16 1277
BssSI C'ACGA_G
Cuts at: 0 1227 1293
Size: 1227 66
BstYI r'GATC_y
Cuts at: 0 322 839 1253 1293
Size: 322 517 414 40
Fragments arranged by size:
517 414 322 40
BtgI C'CryG_G
Cuts at: 0 257 1191 1293
Size: 257 934 102
Fragments arranged by size:
934 257 102
BtsI GCAGTG_nn'
Cuts at: 0 669 1293
Size: 669 624
DrdI GACnn_nn'nnGTC
Cuts at: 0 706 1293
Size: 706 587
EaeI y'GGCC_r
Cuts at: 0 504 581 1293
Size: 504 77 712
Fragments arranged by size:
712 504 77
EarI CTCTTCn'nnn_
Cuts at: 0 338 1268 1293
Size: 338 930 25
Fragments arranged by size:
930 338 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 319 464 856 1079 1225 1293
Size: 283 36 145 392 223 146 68
Fragments arranged by size:
392 283 223 146 145 68 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 784 1293
Size: 784 509
EcoO109I rG'GnC_Cy
Cuts at: 0 521 1132 1293
Size: 521 611 161
Fragments arranged by size:
611 521 161
EcoRI G'AATT_C
Cuts at: 0 547 963 1293
Size: 547 416 330
Fragments arranged by size:
547 416 330
FseI GG_CCGG'CC
Cuts at: 0 267 1293
Size: 267 1026
FspI TGC'GCA
Cuts at: 0 453 866 1293
Size: 453 413 427
Fragments arranged by size:
453 427 413
HaeII r_GCGC'y
Cuts at: 0 665 800 1293
Size: 665 135 493
Fragments arranged by size:
665 493 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1124 1156 1245 1277 1293
Size: 1124 32 89 32 16
Fragments arranged by size:
1124 89 32 32 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 161 761 1293
Size: 146 15 600 532
Fragments arranged by size:
600 532 146 15
MscI TGG'CCA
Cuts at: 0 506 1293
Size: 506 787
MslI CAynn'nnrTG
Cuts at: 0 619 1072 1293
Size: 619 453 221
Fragments arranged by size:
619 453 221
MspA1I CmG'CkG
Cuts at: 0 339 993 1293
Size: 339 654 300
Fragments arranged by size:
654 339 300
NaeI GCC'GGC
Cuts at: 0 265 1293
Size: 265 1028
NcoI C'CATG_G
Cuts at: 0 257 1293
Size: 257 1036
NgoMIV G'CCGG_C
Cuts at: 0 263 1293
Size: 263 1030
NspI r_CATG'y
Cuts at: 0 116 1293
Size: 116 1177
PciI A'CATG_T
Cuts at: 0 112 1293
Size: 112 1181
PfoI T'CCnGG_A
Cuts at: 0 874 1293
Size: 874 419
PpuMI rG'GwC_Cy
Cuts at: 0 1132 1293
Size: 1132 161
PstI C_TGCA'G
Cuts at: 0 367 1293
Size: 367 926
PvuII CAG'CTG
Cuts at: 0 339 993 1293
Size: 339 654 300
Fragments arranged by size:
654 339 300
SanDI GG'GwC_CC
Cuts at: 0 1132 1293
Size: 1132 161
SapI GCTCTTCn'nnn_
Cuts at: 0 338 1293
Size: 338 955
ScaI AGT'ACT
Cuts at: 0 130 1293
Size: 130 1163
SfcI C'TryA_G
Cuts at: 0 363 588 792 1293
Size: 363 225 204 501
Fragments arranged by size:
501 363 225 204
SmlI C'TyrA_G
Cuts at: 0 723 1293
Size: 723 570
StyI C'CwwG_G
Cuts at: 0 257 848 1152 1268 1293
Size: 257 591 304 116 25
Fragments arranged by size:
591 304 257 116 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 1293
Size: 642 651
TatI w'GTAC_w
Cuts at: 0 128 1293
Size: 128 1165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 101 133 844 876 1293
Size: 101 32 711 32 417
Fragments arranged by size:
711 417 101 32 32
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI ApoI
AscI BamHI BanI BanII BcgI BclI BglI BglII
Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI Bsp1286I BspEI BspMI BsrDI
BsrFI BssHII BssSI BstYI BtgI BtsI DrdI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PciI PfoI PpuMI PstI PvuII SanDI SapI
ScaI SfcI SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AlfI AloI AlwNI
ApaI ApaLI AseI AsiSI AvaI AvrII BaeI BbeI
BbsI BbvCI BciVI BfrBI BlpI BmgBI BmtI Bpu10I
BsaI BsaBI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI
BsrBI BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI
EcoRV FalI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI