Restriction map for
pF1KB6593
(Linear) (Six-base) MAPSORT of: KIBB6593.seq Check: 1131 from: 1 to: 1131
KIBB6593.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 779 802 988 1131
Size: 310 469 23 186 143
Fragments arranged by size:
469 310 186 143 23
AflIII A'CryG_T
Cuts at: 0 473 1131
Size: 473 658
AgeI A'CCGG_T
Cuts at: 0 496 1131
Size: 496 635
AleI CACnn'nnGTG
Cuts at: 0 19 1131
Size: 19 1112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 982 1014 1131
Size: 982 32 117
Fragments arranged by size:
982 117 32
AlwNI CAG_nnn'CTG
Cuts at: 0 202 724 1131
Size: 202 522 407
Fragments arranged by size:
522 407 202
ApaI G_GGCC'C
Cuts at: 0 530 1131
Size: 530 601
ApoI r'AATT_y
Cuts at: 0 613 1131
Size: 613 518
AvaI C'yCGr_G
Cuts at: 0 994 1131
Size: 994 137
BamHI G'GATC_C
Cuts at: 0 1035 1131
Size: 1035 96
BanI G'GyrC_C
Cuts at: 0 580 598 1131
Size: 580 18 533
Fragments arranged by size:
580 533 18
BanII G_rGCy'C
Cuts at: 0 530 1072 1131
Size: 530 542 59
Fragments arranged by size:
542 530 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 767 1127 1131
Size: 767 360 4
Fragments arranged by size:
767 360 4
BbvCI CC'TCA_GC
Cuts at: 0 731 1131
Size: 731 400
BciVI GTATCCnnnnn_n'
Cuts at: 0 220 1131
Size: 220 911
BglI GCCn_nnn'nGGC
Cuts at: 0 283 525 709 1084 1131
Size: 283 242 184 375 47
Fragments arranged by size:
375 283 242 184 47
BlpI GC'TnA_GC
Cuts at: 0 933 1131
Size: 933 198
Bme1580I G_kGCm'C
Cuts at: 0 530 1131
Size: 530 601
BmgBI CAC'GTC
Cuts at: 0 981 1131
Size: 981 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 340 524 623 761 827 1131
Size: 340 184 99 138 66 304
Fragments arranged by size:
340 304 184 138 99 66
Bpu10I CC'TnA_GC
Cuts at: 0 641 731 832 1131
Size: 641 90 101 299
Fragments arranged by size:
641 299 101 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1131
Size: 376 755
BsaBI GATnn'nnATC
Cuts at: 0 322 790 1131
Size: 322 468 341
Fragments arranged by size:
468 341 322
BsaWI w'CCGG_w
Cuts at: 0 496 1131
Size: 496 635
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 504 534 1131
Size: 504 30 597
Fragments arranged by size:
597 504 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 709 712 979 1131
Size: 451 258 3 267 152
Fragments arranged by size:
451 267 258 152 3
BseYI C'CCAG_C
Cuts at: 0 83 588 1131
Size: 83 505 543
Fragments arranged by size:
543 505 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 1131
Size: 243 888
Bsp1286I G_dGCh'C
Cuts at: 0 530 1072 1131
Size: 530 542 59
Fragments arranged by size:
542 530 59
BsrDI GCAATG_nn'
Cuts at: 0 326 1131
Size: 326 805
BsrFI r'CCGG_y
Cuts at: 0 496 1131
Size: 496 635
BstAPI GCAn_nnn'nTGC
Cuts at: 0 724 1131
Size: 724 407
BstBI TT'CG_AA
Cuts at: 0 1056 1131
Size: 1056 75
BstYI r'GATC_y
Cuts at: 0 366 624 1035 1131
Size: 366 258 411 96
Fragments arranged by size:
411 366 258 96
EaeI y'GGCC_r
Cuts at: 0 204 810 900 1131
Size: 204 606 90 231
Fragments arranged by size:
606 231 204 90
EarI CTCTTCn'nnn_
Cuts at: 0 428 668 845 1131
Size: 428 240 177 286
Fragments arranged by size:
428 286 240 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 922 1131
Size: 922 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 779 802 988 1131
Size: 310 469 23 186 143
Fragments arranged by size:
469 310 186 143 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 394 1131
Size: 394 737
EcoO109I rG'GnC_Cy
Cuts at: 0 990 1131
Size: 990 141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 270 1131
Size: 270 861
FspI TGC'GCA
Cuts at: 0 322 1131
Size: 322 809
FspAI rTGC'GCAy
Cuts at: 0 322 1131
Size: 322 809
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 504 536 916 948 1131
Size: 504 32 380 32 183
Fragments arranged by size:
504 380 183 32 32
HincII GTy'rAC
Cuts at: 0 99 414 1131
Size: 99 315 717
Fragments arranged by size:
717 315 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1131
Size: 258 873
MscI TGG'CCA
Cuts at: 0 902 1131
Size: 902 229
MslI CAynn'nnrTG
Cuts at: 0 19 271 874 1131
Size: 19 252 603 257
Fragments arranged by size:
603 257 252 19
MspA1I CmG'CkG
Cuts at: 0 681 687 715 1131
Size: 681 6 28 416
Fragments arranged by size:
681 416 28 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 202 1131
Size: 202 929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 486 518 1131
Size: 486 32 613
Fragments arranged by size:
613 486 32
PpuMI rG'GwC_Cy
Cuts at: 0 990 1131
Size: 990 141
PspOMI G'GGCC_C
Cuts at: 0 526 1131
Size: 526 605
PstI C_TGCA'G
Cuts at: 0 80 720 887 1131
Size: 80 640 167 244
Fragments arranged by size:
640 244 167 80
PvuII CAG'CTG
Cuts at: 0 715 1131
Size: 715 416
SapI GCTCTTCn'nnn_
Cuts at: 0 668 845 1131
Size: 668 177 286
Fragments arranged by size:
668 286 177
SbfI CC_TGCA'GG
Cuts at: 0 887 1131
Size: 887 244
SfcI C'TryA_G
Cuts at: 0 76 716 883 1131
Size: 76 640 167 248
Fragments arranged by size:
640 248 167 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1084 1131
Size: 1084 47
SmlI C'TyrA_G
Cuts at: 0 391 1131
Size: 391 740
StyI C'CwwG_G
Cuts at: 0 239 650 959 1091 1131
Size: 239 411 309 132 40
Fragments arranged by size:
411 309 239 132 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 332 364 807 839 1131
Size: 332 32 443 32 292
Fragments arranged by size:
443 332 292 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 805 936 1131
Size: 805 131 195
Fragments arranged by size:
805 195 131
XmnI GAAnn'nnTTC
Cuts at: 0 498 1052 1131
Size: 498 554 79
Fragments arranged by size:
554 498 79
Enzymes that do cut:
AcuI AflIII AgeI AleI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BglI
BlpI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaBI BsaWI
BsaXI BseRI BseYI BsgI Bsp1286I BsrDI BsrFI BstAPI
BstBI BstYI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI FspI FspAI Hin4I HincII MmeI MscI MslI
MspA1I PflMI PpiI PpuMI PspOMI PstI PvuII SapI
SbfI SfcI SfiI SmlI StyI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BclI BfrBI BglII BmtI BplI BpmI BsaI
BsaAI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrGI BssHII BssSI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FseI HaeII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI Tth111I
XbaI XhoI XmaI ZraI