Restriction map for
pF1KB6587
(Linear) (Six-base) MAPSORT of: KIBB6587.seq Check: 9020 from: 1 to: 1065
KIBB6587.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1065
Size: 643 422
AflIII A'CryG_T
Cuts at: 0 804 1065
Size: 804 261
AlwNI CAG_nnn'CTG
Cuts at: 0 147 1065
Size: 147 918
AvaI C'yCGr_G
Cuts at: 0 25 196 1065
Size: 25 171 869
Fragments arranged by size:
869 171 25
BamHI G'GATC_C
Cuts at: 0 234 728 1065
Size: 234 494 337
Fragments arranged by size:
494 337 234
BanII G_rGCy'C
Cuts at: 0 103 401 1065
Size: 103 298 664
Fragments arranged by size:
664 298 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 179 1065
Size: 179 886
BciVI GTATCCnnnnn_n'
Cuts at: 0 702 973 1065
Size: 702 271 92
Fragments arranged by size:
702 271 92
BclI T'GATC_A
Cuts at: 0 1044 1065
Size: 1044 21
BglI GCCn_nnn'nGGC
Cuts at: 0 709 1065
Size: 709 356
Bme1580I G_kGCm'C
Cuts at: 0 491 1065
Size: 491 574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 681 718 1065
Size: 79 602 37 347
Fragments arranged by size:
602 347 79 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1065
Size: 481 584
BsaI GGTCTCn'nnnn_
Cuts at: 0 220 1065
Size: 220 845
BsaHI Gr'CG_yC
Cuts at: 0 990 1065
Size: 990 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 532 1065
Size: 502 30 533
Fragments arranged by size:
533 502 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 214 229 378 381 390 1065
Size: 199 15 15 149 3 9 675
Fragments arranged by size:
675 199 149 15 15 9 3
BseYI C'CCAG_C
Cuts at: 0 37 162 334 718 756 887 1065
Size: 37 125 172 384 38 131 178
Fragments arranged by size:
384 178 172 131 125 38 37
BsiHKAI G_wGCw'C
Cuts at: 0 103 1065
Size: 103 962
Bsp1286I G_dGCh'C
Cuts at: 0 103 401 491 1065
Size: 103 298 90 574
Fragments arranged by size:
574 298 103 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 1065
Size: 47 1018
BsrBI CCG'CTC
Cuts at: 0 453 1065
Size: 453 612
BsrFI r'CCGG_y
Cuts at: 0 116 308 1065
Size: 116 192 757
Fragments arranged by size:
757 192 116
BstXI CCAn_nnnn'nTGG
Cuts at: 0 768 915 1065
Size: 768 147 150
Fragments arranged by size:
768 150 147
BstYI r'GATC_y
Cuts at: 0 234 728 879 1065
Size: 234 494 151 186
Fragments arranged by size:
494 234 186 151
BtgI C'CryG_G
Cuts at: 0 87 491 1065
Size: 87 404 574
Fragments arranged by size:
574 404 87
EaeI y'GGCC_r
Cuts at: 0 206 632 836 920 944 1065
Size: 206 426 204 84 24 121
Fragments arranged by size:
426 206 204 121 84 24
EarI CTCTTCn'nnn_
Cuts at: 0 176 281 355 767 1065
Size: 176 105 74 412 298
Fragments arranged by size:
412 298 176 105 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 387 1065
Size: 387 678
EcoICRI GAG'CTC
Cuts at: 0 101 1065
Size: 101 964
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 643 681 718 1065
Size: 79 564 38 37 347
Fragments arranged by size:
564 347 79 38 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 274 973 1065
Size: 274 699 92
Fragments arranged by size:
699 274 92
EcoO109I rG'GnC_Cy
Cuts at: 0 144 431 753 780 966 1065
Size: 144 287 322 27 186 99
Fragments arranged by size:
322 287 186 144 99 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 205 265 1065
Size: 205 60 800
Fragments arranged by size:
800 205 60
FspI TGC'GCA
Cuts at: 0 961 1065
Size: 961 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 219 251 418 450 502 534 1065
Size: 219 32 167 32 52 32 531
Fragments arranged by size:
531 219 167 52 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 239 836 1065
Size: 41 198 597 229
Fragments arranged by size:
597 229 198 41
MscI TGG'CCA
Cuts at: 0 838 922 946 1065
Size: 838 84 24 119
Fragments arranged by size:
838 119 84 24
MslI CAynn'nnrTG
Cuts at: 0 343 484 670 1065
Size: 343 141 186 395
Fragments arranged by size:
395 343 186 141
MspA1I CmG'CkG
Cuts at: 0 493 1065
Size: 493 572
NaeI GCC'GGC
Cuts at: 0 118 310 1065
Size: 118 192 755
Fragments arranged by size:
755 192 118
NgoMIV G'CCGG_C
Cuts at: 0 116 308 1065
Size: 116 192 757
Fragments arranged by size:
757 192 116
NspI r_CATG'y
Cuts at: 0 669 808 1065
Size: 669 139 257
Fragments arranged by size:
669 257 139
PciI A'CATG_T
Cuts at: 0 804 1065
Size: 804 261
PflMI CCAn_nnn'nTGG
Cuts at: 0 147 1065
Size: 147 918
PfoI T'CCnGG_A
Cuts at: 0 221 269 1065
Size: 221 48 796
Fragments arranged by size:
796 221 48
PpuMI rG'GwC_Cy
Cuts at: 0 144 780 1065
Size: 144 636 285
Fragments arranged by size:
636 285 144
PshAI GACnn'nnGTC
Cuts at: 0 571 989 1065
Size: 571 418 76
Fragments arranged by size:
571 418 76
SacI G_AGCT'C
Cuts at: 0 103 1065
Size: 103 962
SacII CC_GC'GG
Cuts at: 0 494 1065
Size: 494 571
SanDI GG'GwC_CC
Cuts at: 0 780 1065
Size: 780 285
SmlI C'TyrA_G
Cuts at: 0 460 1065
Size: 460 605
SphI G_CATG'C
Cuts at: 0 669 1065
Size: 669 396
SspI AAT'ATT
Cuts at: 0 547 1065
Size: 547 518
StuI AGG'CCT
Cuts at: 0 1056 1065
Size: 1056 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 463 578 890 1065
Size: 463 115 312 175
Fragments arranged by size:
463 312 175 115
TatI w'GTAC_w
Cuts at: 0 440 1065
Size: 440 625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 915 1065
Size: 915 150
Enzymes that do cut:
AcuI AflIII AlwNI AvaI BamHI BanII BbsI BciVI
BclI BglI Bme1580I BpmI BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstXI
BstYI BtgI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI Hin4I MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PciI PflMI PfoI PpuMI PshAI
SacI SacII SanDI SmlI SphI SspI StuI TaqII
TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FseI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StyI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI