Restriction map for
pF1KB6582
(Linear) (Six-base) MAPSORT of: KIBB6582.seq Check: 4121 from: 1 to: 1212
KIBB6582.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1212
Size: 373 839
AhdI GACnn_n'nnGTC
Cuts at: 0 234 1212
Size: 234 978
AlwNI CAG_nnn'CTG
Cuts at: 0 174 1212
Size: 174 1038
ApaI G_GGCC'C
Cuts at: 0 1118 1212
Size: 1118 94
ApoI r'AATT_y
Cuts at: 0 91 1212
Size: 91 1121
AvaI C'yCGr_G
Cuts at: 0 645 1204 1212
Size: 645 559 8
Fragments arranged by size:
645 559 8
BanII G_rGCy'C
Cuts at: 0 29 1118 1212
Size: 29 1089 94
Fragments arranged by size:
1089 94 29
BbvCI CC'TCA_GC
Cuts at: 0 1047 1212
Size: 1047 165
BclI T'GATC_A
Cuts at: 0 429 1212
Size: 429 783
BglII A'GATC_T
Cuts at: 0 839 1212
Size: 839 373
Bme1580I G_kGCm'C
Cuts at: 0 779 1118 1212
Size: 779 339 94
Fragments arranged by size:
779 339 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1167 1212
Size: 1167 45
Bpu10I CC'TnA_GC
Cuts at: 0 1047 1212
Size: 1047 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1212
Size: 431 781
BsaI GGTCTCn'nnnn_
Cuts at: 0 301 1212
Size: 301 911
BsaBI GATnn'nnATC
Cuts at: 0 341 1212
Size: 341 871
BsaHI Gr'CG_yC
Cuts at: 0 218 1212
Size: 218 994
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 1212
Size: 256 956
BseYI C'CCAG_C
Cuts at: 0 135 1212
Size: 135 1077
BsmI GAATG_Cn'
Cuts at: 0 1096 1212
Size: 1096 116
Bsp1286I G_dGCh'C
Cuts at: 0 29 779 1118 1212
Size: 29 750 339 94
Fragments arranged by size:
750 339 94 29
BspHI T'CATG_A
Cuts at: 0 1130 1212
Size: 1130 82
BsrBI CCG'CTC
Cuts at: 0 584 1212
Size: 584 628
BsrGI T'GTAC_A
Cuts at: 0 635 1089 1212
Size: 635 454 123
Fragments arranged by size:
635 454 123
BstEII G'GTnAC_C
Cuts at: 0 1023 1212
Size: 1023 189
BstYI r'GATC_y
Cuts at: 0 14 342 839 1191 1212
Size: 14 328 497 352 21
Fragments arranged by size:
497 352 328 21 14
BtgI C'CryG_G
Cuts at: 0 606 1212
Size: 606 606
BtsI GCAGTG_nn'
Cuts at: 0 104 1212
Size: 104 1108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 939 974 1212
Size: 939 35 238
Fragments arranged by size:
939 238 35
EarI CTCTTCn'nnn_
Cuts at: 0 323 736 1129 1212
Size: 323 413 393 83
Fragments arranged by size:
413 393 323 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1167 1212
Size: 373 794 45
Fragments arranged by size:
794 373 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 121 1212
Size: 121 1091
EcoO109I rG'GnC_Cy
Cuts at: 0 1114 1115 1212
Size: 1114 1 97
Fragments arranged by size:
1114 97 1
EcoRI G'AATT_C
Cuts at: 0 91 1212
Size: 91 1121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 700 1212
Size: 700 512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 281 313 824 856 1212
Size: 220 32 29 32 511 32 356
Fragments arranged by size:
511 356 220 32 32 32 29
HindIII A'AGCT_T
Cuts at: 0 395 1212
Size: 395 817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1041 1212
Size: 218 823 171
Fragments arranged by size:
823 218 171
MspA1I CmG'CkG
Cuts at: 0 986 1164 1212
Size: 986 178 48
Fragments arranged by size:
986 178 48
NspI r_CATG'y
Cuts at: 0 496 1212
Size: 496 716
PflMI CCAn_nnn'nTGG
Cuts at: 0 174 1212
Size: 174 1038
PspOMI G'GGCC_C
Cuts at: 0 1114 1212
Size: 1114 98
PstI C_TGCA'G
Cuts at: 0 44 1212
Size: 44 1168
PvuII CAG'CTG
Cuts at: 0 1164 1212
Size: 1164 48
RsrII CG'GwC_CG
Cuts at: 0 204 1212
Size: 204 1008
ScaI AGT'ACT
Cuts at: 0 106 1003 1212
Size: 106 897 209
Fragments arranged by size:
897 209 106
SfcI C'TryA_G
Cuts at: 0 40 1212
Size: 40 1172
SmlI C'TyrA_G
Cuts at: 0 446 1212
Size: 446 766
StyI C'CwwG_G
Cuts at: 0 346 504 1212
Size: 346 158 708
Fragments arranged by size:
708 346 158
TatI w'GTAC_w
Cuts at: 0 104 635 1001 1089 1212
Size: 104 531 366 88 123
Fragments arranged by size:
531 366 123 104 88
Tth111I GACn'n_nGTC
Cuts at: 0 85 208 1212
Size: 85 123 1004
Fragments arranged by size:
1004 123 85
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 511 1191 1212
Size: 511 680 21
Fragments arranged by size:
680 511 21
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApoI AvaI BanII BbvCI
BclI BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BseRI BseYI BsmI Bsp1286I BspHI BsrBI BsrGI
BstEII BstYI BtgI BtsI CspCI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I HindIII MmeI MspA1I NspI
PflMI PspOMI PstI PvuII RsrII ScaI SfcI SmlI
StyI TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI BsaAI
BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspMI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoRV FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI XbaI XhoI XmaI XmnI ZraI