Restriction map for
pF1KB6572
(Linear) (Six-base) MAPSORT of: KIBB6572.seq Check: 2377 from: 1 to: 1200
KIBB6572.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 0 260 1200
Size: 260 940
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 560 775 1200
Size: 184 376 215 425
Fragments arranged by size:
425 376 215 184
AflIII A'CryG_T
Cuts at: 0 1076 1200
Size: 1076 124
AleI CACnn'nnGTG
Cuts at: 0 241 391 1200
Size: 241 150 809
Fragments arranged by size:
809 241 150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 175 1200
Size: 175 1025
AlwNI CAG_nnn'CTG
Cuts at: 0 773 1200
Size: 773 427
ApaI G_GGCC'C
Cuts at: 0 177 941 1200
Size: 177 764 259
Fragments arranged by size:
764 259 177
ApoI r'AATT_y
Cuts at: 0 288 849 1200
Size: 288 561 351
Fragments arranged by size:
561 351 288
AvaI C'yCGr_G
Cuts at: 0 950 1200
Size: 950 250
BamHI G'GATC_C
Cuts at: 0 665 1200
Size: 665 535
BanI G'GyrC_C
Cuts at: 0 64 1200
Size: 64 1136
BanII G_rGCy'C
Cuts at: 0 177 436 941 1200
Size: 177 259 505 259
Fragments arranged by size:
505 259 259 177
BbsI GAAGACnn'nnnn_
Cuts at: 0 123 847 1200
Size: 123 724 353
Fragments arranged by size:
724 353 123
BclI T'GATC_A
Cuts at: 0 458 1200
Size: 458 742
BglI GCCn_nnn'nGGC
Cuts at: 0 73 1200
Size: 73 1127
BlpI GC'TnA_GC
Cuts at: 0 880 1200
Size: 880 320
Bme1580I G_kGCm'C
Cuts at: 0 177 941 1200
Size: 177 764 259
Fragments arranged by size:
764 259 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 769 1200
Size: 286 483 431
Fragments arranged by size:
483 431 286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1200
Size: 1168 32
BsaAI yAC'GTr
Cuts at: 0 1077 1119 1200
Size: 1077 42 81
Fragments arranged by size:
1077 81 42
BsaWI w'CCGG_w
Cuts at: 0 1169 1200
Size: 1169 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 596 626 714 744 1200
Size: 596 30 88 30 456
Fragments arranged by size:
596 456 88 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 830 833 850 1200
Size: 830 3 17 350
Fragments arranged by size:
830 350 17 3
BseYI C'CCAG_C
Cuts at: 0 933 1110 1200
Size: 933 177 90
Fragments arranged by size:
933 177 90
BsiEI CG_ry'CG
Cuts at: 0 467 1200
Size: 467 733
BsiHKAI G_wGCw'C
Cuts at: 0 436 1200
Size: 436 764
BsmBI CGTCTCn'nnnn_
Cuts at: 0 648 1200
Size: 648 552
Bsp1286I G_dGCh'C
Cuts at: 0 177 436 941 1200
Size: 177 259 505 259
Fragments arranged by size:
505 259 259 177
BspEI T'CCGG_A
Cuts at: 0 1169 1200
Size: 1169 31
BspHI T'CATG_A
Cuts at: 0 332 455 1200
Size: 332 123 745
Fragments arranged by size:
745 332 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 602 1200
Size: 602 598
BsrBI CCG'CTC
Cuts at: 0 171 1200
Size: 171 1029
BsrDI GCAATG_nn'
Cuts at: 0 144 912 1200
Size: 144 768 288
Fragments arranged by size:
768 288 144
BsrGI T'GTAC_A
Cuts at: 0 340 1200
Size: 340 860
BssHII G'CGCG_C
Cuts at: 0 110 1200
Size: 110 1090
BstBI TT'CG_AA
Cuts at: 0 894 1200
Size: 894 306
BstXI CCAn_nnnn'nTGG
Cuts at: 0 22 1200
Size: 22 1178
BstYI r'GATC_y
Cuts at: 0 665 1187 1200
Size: 665 522 13
Fragments arranged by size:
665 522 13
BstZ17I GTA'TAC
Cuts at: 0 261 1200
Size: 261 939
BtsI GCAGTG_nn'
Cuts at: 0 386 398 782 1059 1200
Size: 386 12 384 277 141
Fragments arranged by size:
386 384 277 141 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 535 683 1200
Size: 535 148 517
Fragments arranged by size:
535 517 148
EaeI y'GGCC_r
Cuts at: 0 24 122 233 464 992 1200
Size: 24 98 111 231 528 208
Fragments arranged by size:
528 231 208 111 98 24
EagI C'GGCC_G
Cuts at: 0 464 1200
Size: 464 736
EarI CTCTTCn'nnn_
Cuts at: 0 506 579 1200
Size: 506 73 621
Fragments arranged by size:
621 506 73
EcoICRI GAG'CTC
Cuts at: 0 434 1200
Size: 434 766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 286 560 769 775 1200
Size: 184 102 274 209 6 425
Fragments arranged by size:
425 274 209 184 102 6
EcoO109I rG'GnC_Cy
Cuts at: 0 174 528 937 938 1007 1200
Size: 174 354 409 1 69 193
Fragments arranged by size:
409 354 193 174 69 1
EcoRI G'AATT_C
Cuts at: 0 849 1200
Size: 849 351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 900 1200
Size: 900 300
HaeII r_GCGC'y
Cuts at: 0 874 1130 1200
Size: 874 256 70
Fragments arranged by size:
874 256 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 423 455 596 628 657 689 1200
Size: 423 32 141 32 29 32 511
Fragments arranged by size:
511 423 141 32 32 32 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 1200
Size: 575 625
MscI TGG'CCA
Cuts at: 0 26 124 235 994 1200
Size: 26 98 111 759 206
Fragments arranged by size:
759 206 111 98 26
MslI CAynn'nnrTG
Cuts at: 0 157 241 391 1200
Size: 157 84 150 809
Fragments arranged by size:
809 157 150 84
MspA1I CmG'CkG
Cuts at: 0 18 1200
Size: 18 1182
NspI r_CATG'y
Cuts at: 0 88 1073 1200
Size: 88 985 127
Fragments arranged by size:
985 127 88
PasI CC'CwG_GG
Cuts at: 0 251 1200
Size: 251 949
PflMI CCAn_nnn'nTGG
Cuts at: 0 990 1106 1200
Size: 990 116 94
Fragments arranged by size:
990 116 94
PmlI CAC'GTG
Cuts at: 0 1077 1200
Size: 1077 123
PpuMI rG'GwC_Cy
Cuts at: 0 528 1200
Size: 528 672
PspOMI G'GGCC_C
Cuts at: 0 173 937 1200
Size: 173 764 263
Fragments arranged by size:
764 263 173
PstI C_TGCA'G
Cuts at: 0 182 393 1200
Size: 182 211 807
Fragments arranged by size:
807 211 182
PvuII CAG'CTG
Cuts at: 0 18 1200
Size: 18 1182
SacI G_AGCT'C
Cuts at: 0 436 1200
Size: 436 764
SanDI GG'GwC_CC
Cuts at: 0 528 1200
Size: 528 672
SfcI C'TryA_G
Cuts at: 0 178 389 765 1200
Size: 178 211 376 435
Fragments arranged by size:
435 376 211 178
SmlI C'TyrA_G
Cuts at: 0 1183 1200
Size: 1183 17
StuI AGG'CCT
Cuts at: 0 705 1200
Size: 705 495
StyI C'CwwG_G
Cuts at: 0 239 788 1200
Size: 239 549 412
Fragments arranged by size:
549 412 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 243 781 1200
Size: 243 538 419
Fragments arranged by size:
538 419 243
TatI w'GTAC_w
Cuts at: 0 340 1200
Size: 340 860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 633 1107 1200
Size: 633 474 93
Fragments arranged by size:
633 474 93
XmnI GAAnn'nnTTC
Cuts at: 0 1177 1200
Size: 1177 23
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BclI BglI BlpI
Bme1580I BpmI BpuEI BsaAI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BstBI BstXI BstYI BstZ17I BtsI
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I MmeI MscI MslI MspA1I NspI
PasI PflMI PmlI PpuMI PspOMI PstI PvuII SacI
SanDI SfcI SmlI StuI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BfrBI BglII BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaBI BsaHI BsgI BsiWI BsmI
BsrFI BssSI BstAPI BstEII Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII EciI EcoNI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI