Restriction map for
pF1KB6569
(Linear) (Six-base) MAPSORT of: KIBB6569.seq Check: 3312 from: 1 to: 1125
KIBB6569.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 45 211 553 837 1125
Size: 45 166 342 284 288
Fragments arranged by size:
342 288 284 166 45
ApaI G_GGCC'C
Cuts at: 0 175 257 428 1014 1125
Size: 175 82 171 586 111
Fragments arranged by size:
586 175 171 111 82
ApaLI G'TGCA_C
Cuts at: 0 840 1125
Size: 840 285
AvaI C'yCGr_G
Cuts at: 0 176 600 1125
Size: 176 424 525
Fragments arranged by size:
525 424 176
BanI G'GyrC_C
Cuts at: 0 442 586 1125
Size: 442 144 539
Fragments arranged by size:
539 442 144
BanII G_rGCy'C
Cuts at: 0 175 257 428 463 758 1014 1025 1125
Size: 175 82 171 35 295 256 11 100
Fragments arranged by size:
295 256 175 171 100 82 35 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 76 1125
Size: 76 1049
BglI GCCn_nnn'nGGC
Cuts at: 0 179 485 1125
Size: 179 306 640
Fragments arranged by size:
640 306 179
BglII A'GATC_T
Cuts at: 0 1058 1125
Size: 1058 67
Bme1580I G_kGCm'C
Cuts at: 0 175 257 428 447 844 1014 1125
Size: 175 82 171 19 397 170 111
Fragments arranged by size:
397 175 171 170 111 82 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 89 241 314 1017 1125
Size: 89 152 73 703 108
Fragments arranged by size:
703 152 108 89 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 578 650 706 1125
Size: 305 273 72 56 419
Fragments arranged by size:
419 305 273 72 56
Bpu10I CC'TnA_GC
Cuts at: 0 937 1125
Size: 937 188
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 704 734 1125
Size: 704 30 391
Fragments arranged by size:
704 391 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 4 1125
Size: 4 1121
BseYI C'CCAG_C
Cuts at: 0 421 1125
Size: 421 704
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 1125
Size: 5 1120
BsiHKAI G_wGCw'C
Cuts at: 0 758 844 1125
Size: 758 86 281
Fragments arranged by size:
758 281 86
BsmI GAATG_Cn'
Cuts at: 0 623 894 1125
Size: 623 271 231
Fragments arranged by size:
623 271 231
Bsp1286I G_dGCh'C
Cuts at: 0 175 257 428 447 463 758 844 1014
Size: 175 82 171 19 16 295 86 170
Cuts at: 1014 1025 1125
Size: 11 100
Fragments arranged by size:
295 175 171 170 100 86 82 19
16 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 678 1125
Size: 678 447
BstAPI GCAn_nnn'nTGC
Cuts at: 0 553 1125
Size: 553 572
BstYI r'GATC_y
Cuts at: 0 1058 1125
Size: 1058 67
Bsu36I CC'TnA_GG
Cuts at: 0 392 491 1125
Size: 392 99 634
Fragments arranged by size:
634 392 99
BtgI C'CryG_G
Cuts at: 0 563 1125
Size: 563 562
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 164 1125
Size: 164 961
EaeI y'GGCC_r
Cuts at: 0 768 1125
Size: 768 357
EarI CTCTTCn'nnn_
Cuts at: 0 13 348 763 1125
Size: 13 335 415 362
Fragments arranged by size:
415 362 335 13
EcoICRI GAG'CTC
Cuts at: 0 756 1125
Size: 756 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 578 650 706 1125
Size: 305 273 72 56 419
Fragments arranged by size:
419 305 273 72 56
EcoO109I rG'GnC_Cy
Cuts at: 0 171 172 192 325 361 388 487 568
Size: 171 1 20 133 36 27 99 81
Cuts at: 568 595 1011 1125
Size: 27 416 114
Fragments arranged by size:
416 171 133 114 99 81 36 27
27 20 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 1024 1056 1125
Size: 256 32 736 32 69
Fragments arranged by size:
736 256 69 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1028 1125
Size: 258 770 97
Fragments arranged by size:
770 258 97
MscI TGG'CCA
Cuts at: 0 770 1125
Size: 770 355
MspA1I CmG'CkG
Cuts at: 0 349 834 883 906 1125
Size: 349 485 49 23 219
Fragments arranged by size:
485 349 219 49 23
NcoI C'CATG_G
Cuts at: 0 563 1125
Size: 563 562
NruI TCG'CGA
Cuts at: 0 149 1125
Size: 149 976
NspI r_CATG'y
Cuts at: 0 456 1125
Size: 456 669
PasI CC'CwG_GG
Cuts at: 0 45 366 573 1015 1125
Size: 45 321 207 442 110
Fragments arranged by size:
442 321 207 110 45
PfoI T'CCnGG_A
Cuts at: 0 321 627 1125
Size: 321 306 498
Fragments arranged by size:
498 321 306
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 952 984 1125
Size: 952 32 141
Fragments arranged by size:
952 141 32
PpuMI rG'GwC_Cy
Cuts at: 0 192 325 361 388 568 595 1125
Size: 192 133 36 27 180 27 530
Fragments arranged by size:
530 192 180 133 36 27 27
PspOMI G'GGCC_C
Cuts at: 0 171 253 424 1010 1125
Size: 171 82 171 586 115
Fragments arranged by size:
586 171 171 115 82
PstI C_TGCA'G
Cuts at: 0 216 1125
Size: 216 909
PvuII CAG'CTG
Cuts at: 0 349 834 906 1125
Size: 349 485 72 219
Fragments arranged by size:
485 349 219 72
SacI G_AGCT'C
Cuts at: 0 758 1125
Size: 758 367
SanDI GG'GwC_CC
Cuts at: 0 192 568 595 1125
Size: 192 376 27 530
Fragments arranged by size:
530 376 192 27
SapI GCTCTTCn'nnn_
Cuts at: 0 13 763 1125
Size: 13 750 362
Fragments arranged by size:
750 362 13
SfcI C'TryA_G
Cuts at: 0 212 1125
Size: 212 913
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 485 1125
Size: 485 640
SmaI CCC'GGG
Cuts at: 0 602 1125
Size: 602 523
SphI G_CATG'C
Cuts at: 0 456 1125
Size: 456 669
StuI AGG'CCT
Cuts at: 0 479 697 1125
Size: 479 218 428
Fragments arranged by size:
479 428 218
StyI C'CwwG_G
Cuts at: 0 563 1125
Size: 563 562
TatI w'GTAC_w
Cuts at: 0 711 1125
Size: 711 414
XmaI C'CCGG_G
Cuts at: 0 600 1125
Size: 600 525
XmnI GAAnn'nnTTC
Cuts at: 0 790 1125
Size: 790 335
Enzymes that do cut:
AlwNI ApaI ApaLI AvaI BanI BanII BbsI BglI
BglII Bme1580I BmrI BpmI Bpu10I BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BstAPI BstYI Bsu36I
BtgI BtgZI EaeI EarI EcoICRI Eco57MI EcoO109I Hin4I
MmeI MscI MspA1I NcoI NruI NspI PasI PfoI
PpiI PpuMI PspOMI PstI PvuII SacI SanDI SapI
SfcI SfiI SmaI SphI StuI StyI TatI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BlpI BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV
NheI NotI NsiI PacI PciI PflMI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI ZraI