Restriction map for
pF1KB6565
(Linear) (Six-base) MAPSORT of: KIBB6565.seq Check: 5898 from: 1 to: 1122
KIBB6565.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 690 1122
Size: 690 432
Acc65I G'GTAC_C
Cuts at: 0 389 1122
Size: 389 733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 619 781 1122
Size: 499 120 162 341
Fragments arranged by size:
499 341 162 120
AfeI AGC'GCT
Cuts at: 0 817 1122
Size: 817 305
ApaI G_GGCC'C
Cuts at: 0 791 1122
Size: 791 331
AvaI C'yCGr_G
Cuts at: 0 132 878 1122
Size: 132 746 244
Fragments arranged by size:
746 244 132
BamHI G'GATC_C
Cuts at: 0 81 323 1122
Size: 81 242 799
Fragments arranged by size:
799 242 81
BanI G'GyrC_C
Cuts at: 0 73 389 735 1122
Size: 73 316 346 387
Fragments arranged by size:
387 346 316 73
BanII G_rGCy'C
Cuts at: 0 377 512 791 1122
Size: 377 135 279 331
Fragments arranged by size:
377 331 279 135
BbeI G_GCGC'C
Cuts at: 0 77 1122
Size: 77 1045
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 787 882 1122
Size: 105 682 95 240
Fragments arranged by size:
682 240 105 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 960 994 1122
Size: 960 34 128
Fragments arranged by size:
960 128 34
BglII A'GATC_T
Cuts at: 0 729 1122
Size: 729 393
Bme1580I G_kGCm'C
Cuts at: 0 791 1122
Size: 791 331
BmrI ACTGGGnnnn_n'
Cuts at: 0 99 1122
Size: 99 1023
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 396 1122
Size: 396 726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 732 1122
Size: 49 683 390
Fragments arranged by size:
683 390 49
Bpu10I CC'TnA_GC
Cuts at: 0 124 1122
Size: 124 998
BsaBI GATnn'nnATC
Cuts at: 0 526 1122
Size: 526 596
BsaHI Gr'CG_yC
Cuts at: 0 74 110 188 236 869 1122
Size: 74 36 78 48 633 253
Fragments arranged by size:
633 253 78 74 48 36
BsaWI w'CCGG_w
Cuts at: 0 143 809 1122
Size: 143 666 313
Fragments arranged by size:
666 313 143
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 1122
Size: 79 30 1013
Fragments arranged by size:
1013 79 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 1122
Size: 211 911
BsiEI CG_ry'CG
Cuts at: 0 46 993 1122
Size: 46 947 129
Fragments arranged by size:
947 129 46
BsiHKAI G_wGCw'C
Cuts at: 0 477 536 1122
Size: 477 59 586
Fragments arranged by size:
586 477 59
BsmI GAATG_Cn'
Cuts at: 0 706 1122
Size: 706 416
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1009 1122
Size: 1009 113
Bsp1286I G_dGCh'C
Cuts at: 0 377 477 512 536 791 1122
Size: 377 100 35 24 255 331
Fragments arranged by size:
377 331 255 100 35 24
BspEI T'CCGG_A
Cuts at: 0 809 1122
Size: 809 313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 576 690 704 1122
Size: 576 114 14 418
Fragments arranged by size:
576 418 114 14
BsrBI CCG'CTC
Cuts at: 0 12 1122
Size: 12 1110
BsrFI r'CCGG_y
Cuts at: 0 281 555 1122
Size: 281 274 567
Fragments arranged by size:
567 281 274
BssHII G'CGCG_C
Cuts at: 0 55 1122
Size: 55 1067
BstEII G'GTnAC_C
Cuts at: 0 588 1122
Size: 588 534
BstYI r'GATC_y
Cuts at: 0 81 323 729 1122
Size: 81 242 406 393
Fragments arranged by size:
406 393 242 81
BtgI C'CryG_G
Cuts at: 0 225 576 827 1122
Size: 225 351 251 295
Fragments arranged by size:
351 295 251 225
BtsI GCAGTG_nn'
Cuts at: 0 325 1100 1122
Size: 325 775 22
Fragments arranged by size:
775 325 22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 891 926 1122
Size: 891 35 196
Fragments arranged by size:
891 196 35
EaeI y'GGCC_r
Cuts at: 0 2 43 417 557 567 961 1122
Size: 2 41 374 140 10 394 161
Fragments arranged by size:
394 374 161 140 41 10 2
EagI C'GGCC_G
Cuts at: 0 43 1122
Size: 43 1079
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 1122
Size: 169 953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 499 619 732 781 1122
Size: 49 450 120 113 49 341
Fragments arranged by size:
450 341 120 113 49 49
EcoO109I rG'GnC_Cy
Cuts at: 0 787 1122
Size: 787 335
HaeII r_GCGC'y
Cuts at: 0 77 819 1122
Size: 77 742 303
Fragments arranged by size:
742 303 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 872 904 1006 1038 1122
Size: 79 32 761 32 102 32 84
Fragments arranged by size:
761 102 84 79 32 32 32
KasI G'GCGC_C
Cuts at: 0 73 1122
Size: 73 1049
KpnI G_GTAC'C
Cuts at: 0 393 1122
Size: 393 729
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 1049 1122
Size: 320 729 73
Fragments arranged by size:
729 320 73
MscI TGG'CCA
Cuts at: 0 4 963 1122
Size: 4 959 159
Fragments arranged by size:
959 159 4
MspA1I CmG'CkG
Cuts at: 0 42 1122
Size: 42 1080
NaeI GCC'GGC
Cuts at: 0 557 1122
Size: 557 565
NarI GG'CG_CC
Cuts at: 0 74 1122
Size: 74 1048
NcoI C'CATG_G
Cuts at: 0 827 1122
Size: 827 295
NgoMIV G'CCGG_C
Cuts at: 0 555 1122
Size: 555 567
PasI CC'CwG_GG
Cuts at: 0 712 948 1122
Size: 712 236 174
Fragments arranged by size:
712 236 174
PfoI T'CCnGG_A
Cuts at: 0 26 748 1122
Size: 26 722 374
Fragments arranged by size:
722 374 26
PspOMI G'GGCC_C
Cuts at: 0 787 1122
Size: 787 335
PstI C_TGCA'G
Cuts at: 0 41 587 701 1122
Size: 41 546 114 421
Fragments arranged by size:
546 421 114 41
SbfI CC_TGCA'GG
Cuts at: 0 587 701 1122
Size: 587 114 421
Fragments arranged by size:
587 421 114
SfcI C'TryA_G
Cuts at: 0 37 583 697 1122
Size: 37 546 114 425
Fragments arranged by size:
546 425 114 37
SfoI GGC'GCC
Cuts at: 0 75 1122
Size: 75 1047
StyI C'CwwG_G
Cuts at: 0 5 503 512 827 1122
Size: 5 498 9 315 295
Fragments arranged by size:
498 315 295 9 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 961 1029 1122
Size: 961 68 93
Fragments arranged by size:
961 93 68
TatI w'GTAC_w
Cuts at: 0 848 1122
Size: 848 274
Tth111I GACn'n_nGTC
Cuts at: 0 1020 1122
Size: 1020 102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 964 1122
Size: 964 158
XmnI GAAnn'nnTTC
Cuts at: 0 484 1122
Size: 484 638
Enzymes that do cut:
AarI Acc65I AcuI AfeI ApaI AvaI BamHI BanI
BanII BbeI BbsI BcgI BglII Bme1580I BmrI BplI
BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BssHII BstEII BstYI BtgI BtsI CspCI EaeI EagI
EciI Eco57MI EcoO109I HaeII Hin4I KasI KpnI MmeI
MscI MspA1I NaeI NarI NcoI NgoMIV PasI PfoI
PspOMI PstI SbfI SfcI SfoI StyI TaqII TatI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbvCI BciVI BclI BfrBI BglI BlpI
BmgBI BmtI BpuEI BsaI BsaAI BseYI BsgI BsiWI
BspHI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EarI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI MfeI MluI MslI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI XbaI XhoI XmaI
ZraI