Restriction map for
pF1KB6563
(Linear) (Six-base) MAPSORT of: KIBB6563.seq Check: 8817 from: 1 to: 1122
KIBB6563.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 473 1122
Size: 473 649
AatII G_ACGT'C
Cuts at: 0 626 1122
Size: 626 496
AccI GT'mk_AC
Cuts at: 0 245 1122
Size: 245 877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1002 1122
Size: 148 854 120
Fragments arranged by size:
854 148 120
AfeI AGC'GCT
Cuts at: 0 388 1122
Size: 388 734
AflIII A'CryG_T
Cuts at: 0 941 1030 1122
Size: 941 89 92
Fragments arranged by size:
941 92 89
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 574 1122
Size: 574 548
AlwNI CAG_nnn'CTG
Cuts at: 0 411 723 1122
Size: 411 312 399
Fragments arranged by size:
411 399 312
ApaI G_GGCC'C
Cuts at: 0 148 901 1122
Size: 148 753 221
Fragments arranged by size:
753 221 148
ApoI r'AATT_y
Cuts at: 0 457 1122
Size: 457 665
AvaI C'yCGr_G
Cuts at: 0 65 319 979 1122
Size: 65 254 660 143
Fragments arranged by size:
660 254 143 65
BamHI G'GATC_C
Cuts at: 0 89 656 1122
Size: 89 567 466
Fragments arranged by size:
567 466 89
BanI G'GyrC_C
Cuts at: 0 35 199 1122
Size: 35 164 923
Fragments arranged by size:
923 164 35
BanII G_rGCy'C
Cuts at: 0 148 901 1122
Size: 148 753 221
Fragments arranged by size:
753 221 148
BbeI G_GCGC'C
Cuts at: 0 203 1122
Size: 203 919
BbsI GAAGACnn'nnnn_
Cuts at: 0 1056 1122
Size: 1056 66
BbvCI CC'TCA_GC
Cuts at: 0 914 1122
Size: 914 208
BglI GCCn_nnn'nGGC
Cuts at: 0 983 1122
Size: 983 139
Bme1580I G_kGCm'C
Cuts at: 0 148 901 1122
Size: 148 753 221
Fragments arranged by size:
753 221 148
BmgBI CAC'GTC
Cuts at: 0 543 1074 1122
Size: 543 531 48
Fragments arranged by size:
543 531 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 881 1122
Size: 881 241
BmtI G_CTAG'C
Cuts at: 0 341 1122
Size: 341 781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 745 865 1122
Size: 520 225 120 257
Fragments arranged by size:
520 257 225 120
Bpu10I CC'TnA_GC
Cuts at: 0 536 902 914 1122
Size: 536 366 12 208
Fragments arranged by size:
536 366 208 12
BsaBI GATnn'nnATC
Cuts at: 0 187 1122
Size: 187 935
BsaHI Gr'CG_yC
Cuts at: 0 200 623 1011 1122
Size: 200 423 388 111
Fragments arranged by size:
423 388 200 111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 574 604 1122
Size: 574 30 518
Fragments arranged by size:
574 518 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 229 232 529 742 862 1122
Size: 229 3 297 213 120 260
Fragments arranged by size:
297 260 229 213 120 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1122
Size: 616 506
BsiHKAI G_wGCw'C
Cuts at: 0 169 548 1091 1122
Size: 169 379 543 31
Fragments arranged by size:
543 379 169 31
Bsp1286I G_dGCh'C
Cuts at: 0 148 169 548 901 1091 1122
Size: 148 21 379 353 190 31
Fragments arranged by size:
379 353 190 148 31 21
BspHI T'CATG_A
Cuts at: 0 347 1122
Size: 347 775
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 473 617 1122
Size: 473 144 505
Fragments arranged by size:
505 473 144
BsrBI CCG'CTC
Cuts at: 0 8 158 294 1122
Size: 8 150 136 828
Fragments arranged by size:
828 150 136 8
BsrFI r'CCGG_y
Cuts at: 0 202 424 1122
Size: 202 222 698
Fragments arranged by size:
698 222 202
BsrGI T'GTAC_A
Cuts at: 0 944 956 1122
Size: 944 12 166
Fragments arranged by size:
944 166 12
BssSI C'ACGA_G
Cuts at: 0 948 1122
Size: 948 174
BstYI r'GATC_y
Cuts at: 0 89 656 1122
Size: 89 567 466
Fragments arranged by size:
567 466 89
BtgI C'CryG_G
Cuts at: 0 117 195 287 1122
Size: 117 78 92 835
Fragments arranged by size:
835 117 92 78
BtsI GCAGTG_nn'
Cuts at: 0 412 1122
Size: 412 710
DraIII CAC_nnn'GTG
Cuts at: 0 706 965 1122
Size: 706 259 157
Fragments arranged by size:
706 259 157
EaeI y'GGCC_r
Cuts at: 0 398 1122
Size: 398 724
EarI CTCTTCn'nnn_
Cuts at: 0 157 1021 1122
Size: 157 864 101
Fragments arranged by size:
864 157 101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 520 745 865 1002 1122
Size: 148 372 225 120 137 120
Fragments arranged by size:
372 225 148 137 120 120
EcoNI CCTnn'n_nnAGG
Cuts at: 0 43 1122
Size: 43 1079
EcoO109I rG'GnC_Cy
Cuts at: 0 357 897 898 1122
Size: 357 540 1 224
Fragments arranged by size:
540 357 224 1
EcoRI G'AATT_C
Cuts at: 0 457 1122
Size: 457 665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 186 1122
Size: 186 936
HaeII r_GCGC'y
Cuts at: 0 24 203 390 1122
Size: 24 179 187 732
Fragments arranged by size:
732 187 179 24
KasI G'GCGC_C
Cuts at: 0 199 1122
Size: 199 923
MslI CAynn'nnrTG
Cuts at: 0 259 952 1122
Size: 259 693 170
Fragments arranged by size:
693 259 170
MspA1I CmG'CkG
Cuts at: 0 119 1122
Size: 119 1003
NaeI GCC'GGC
Cuts at: 0 204 426 1122
Size: 204 222 696
Fragments arranged by size:
696 222 204
NarI GG'CG_CC
Cuts at: 0 200 1122
Size: 200 922
NcoI C'CATG_G
Cuts at: 0 287 1122
Size: 287 835
NgoMIV G'CCGG_C
Cuts at: 0 202 424 1122
Size: 202 222 698
Fragments arranged by size:
698 222 202
NheI G'CTAG_C
Cuts at: 0 337 1122
Size: 337 785
NspI r_CATG'y
Cuts at: 0 561 945 1034 1122
Size: 561 384 89 88
Fragments arranged by size:
561 384 89 88
PciI A'CATG_T
Cuts at: 0 941 1030 1122
Size: 941 89 92
Fragments arranged by size:
941 92 89
PflMI CCAn_nnn'nTGG
Cuts at: 0 407 723 1122
Size: 407 316 399
Fragments arranged by size:
407 399 316
PfoI T'CCnGG_A
Cuts at: 0 782 842 935 1122
Size: 782 60 93 187
Fragments arranged by size:
782 187 93 60
PpuMI rG'GwC_Cy
Cuts at: 0 357 1122
Size: 357 765
PspOMI G'GGCC_C
Cuts at: 0 144 897 1122
Size: 144 753 225
Fragments arranged by size:
753 225 144
PstI C_TGCA'G
Cuts at: 0 302 1122
Size: 302 820
SacII CC_GC'GG
Cuts at: 0 120 1122
Size: 120 1002
SapI GCTCTTCn'nnn_
Cuts at: 0 157 1122
Size: 157 965
ScaI AGT'ACT
Cuts at: 0 583 1122
Size: 583 539
SfcI C'TryA_G
Cuts at: 0 298 1122
Size: 298 824
SfoI GGC'GCC
Cuts at: 0 201 1122
Size: 201 921
SmaI CCC'GGG
Cuts at: 0 67 1122
Size: 67 1055
SphI G_CATG'C
Cuts at: 0 561 1122
Size: 561 561
StyI C'CwwG_G
Cuts at: 0 287 1122
Size: 287 835
TatI w'GTAC_w
Cuts at: 0 482 581 944 956 1122
Size: 482 99 363 12 166
Fragments arranged by size:
482 363 166 99 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1037 1069 1122
Size: 1037 32 53
Fragments arranged by size:
1037 53 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 867 1122
Size: 867 255
XmaI C'CCGG_G
Cuts at: 0 65 1122
Size: 65 1057
XmnI GAAnn'nnTTC
Cuts at: 0 256 1122
Size: 256 866
ZraI GAC'GTC
Cuts at: 0 624 1122
Size: 624 498
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AlfI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BglI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrGI BssSI BstYI BtgI BtsI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII KasI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PflMI PfoI PpuMI PspOMI PstI
SacII SapI ScaI SfcI SfoI SmaI SphI StyI
TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AleI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI
BfrBI BglII BlpI BplI BpuEI BsaI BsaAI BsaWI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BsrDI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoRV FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NdeI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XhoI