Restriction map for
pF1KB6560
(Linear) (Six-base) MAPSORT of: KIBB6560.seq Check: 7319 from: 1 to: 1026
KIBB6560.orf
With 182 enzymes: SgfI *
July 28, 2009 13:24 ..
AfeI AGC'GCT
Cuts at: 0 114 1026
Size: 114 912
AflIII A'CryG_T
Cuts at: 0 69 135 1026
Size: 69 66 891
Fragments arranged by size:
891 69 66
AleI CACnn'nnGTG
Cuts at: 0 74 1026
Size: 74 952
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 485 517 1026
Size: 485 32 509
Fragments arranged by size:
509 485 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1018 1026
Size: 1018 8
ApaI G_GGCC'C
Cuts at: 0 400 1026
Size: 400 626
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 863 896 1026
Size: 863 33 130
Fragments arranged by size:
863 130 33
BanI G'GyrC_C
Cuts at: 0 120 327 1026
Size: 120 207 699
Fragments arranged by size:
699 207 120
BanII G_rGCy'C
Cuts at: 0 218 400 451 908 1026
Size: 218 182 51 457 118
Fragments arranged by size:
457 218 182 118 51
BclI T'GATC_A
Cuts at: 0 347 1026
Size: 347 679
BlpI GC'TnA_GC
Cuts at: 0 902 1026
Size: 902 124
Bme1580I G_kGCm'C
Cuts at: 0 69 400 515 1026
Size: 69 331 115 511
Fragments arranged by size:
511 331 115 69
Bpu10I CC'TnA_GC
Cuts at: 0 212 1026
Size: 212 814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1026
Size: 110 916
BsaI GGTCTCn'nnnn_
Cuts at: 0 565 852 1026
Size: 565 287 174
Fragments arranged by size:
565 287 174
BsaAI yAC'GTr
Cuts at: 0 72 138 1026
Size: 72 66 888
Fragments arranged by size:
888 72 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 122 152 292 322 407 437 485 515
Size: 122 30 140 30 85 30 48 30
Cuts at: 515 1026
Size: 511
Fragments arranged by size:
511 140 122 85 48 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 864 1026
Size: 864 162
BseYI C'CCAG_C
Cuts at: 0 109 264 331 514 989 1026
Size: 109 155 67 183 475 37
Fragments arranged by size:
475 183 155 109 67 37
BsiEI CG_ry'CG
Cuts at: 0 394 1026
Size: 394 632
BsiHKAI G_wGCw'C
Cuts at: 0 218 451 935 1026
Size: 218 233 484 91
Fragments arranged by size:
484 233 218 91
Bsp1286I G_dGCh'C
Cuts at: 0 69 218 400 451 515 908 935 1026
Size: 69 149 182 51 64 393 27 91
Fragments arranged by size:
393 182 149 91 69 64 51 27
BsrGI T'GTAC_A
Cuts at: 0 185 1026
Size: 185 841
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 1026
Size: 117 909
BstYI r'GATC_y
Cuts at: 0 601 955 1026
Size: 601 354 71
Fragments arranged by size:
601 354 71
Bsu36I CC'TnA_GG
Cuts at: 0 779 1026
Size: 779 247
BtgI C'CryG_G
Cuts at: 0 14 401 564 597 1026
Size: 14 387 163 33 429
Fragments arranged by size:
429 387 163 33 14
BtsI GCAGTG_nn'
Cuts at: 0 515 634 807 1026
Size: 515 119 173 219
Fragments arranged by size:
515 219 173 119
DraIII CAC_nnn'GTG
Cuts at: 0 320 1026
Size: 320 706
EaeI y'GGCC_r
Cuts at: 0 391 1026
Size: 391 635
EagI C'GGCC_G
Cuts at: 0 391 1026
Size: 391 635
EarI CTCTTCn'nnn_
Cuts at: 0 149 1026
Size: 149 877
EcoICRI GAG'CTC
Cuts at: 0 216 449 1026
Size: 216 233 577
Fragments arranged by size:
577 233 216
EcoNI CCTnn'n_nnAGG
Cuts at: 0 128 1026
Size: 128 898
EcoO109I rG'GnC_Cy
Cuts at: 0 201 278 397 553 1026
Size: 201 77 119 156 473
Fragments arranged by size:
473 201 156 119 77
HaeII r_GCGC'y
Cuts at: 0 116 1026
Size: 116 910
HindIII A'AGCT_T
Cuts at: 0 693 1026
Size: 693 333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1026
Size: 782 244
MslI CAynn'nnrTG
Cuts at: 0 74 1026
Size: 74 952
MspA1I CmG'CkG
Cuts at: 0 1018 1026
Size: 1018 8
NcoI C'CATG_G
Cuts at: 0 597 1026
Size: 597 429
NspI r_CATG'y
Cuts at: 0 35 1026
Size: 35 991
PflMI CCAn_nnn'nTGG
Cuts at: 0 320 1018 1026
Size: 320 698 8
Fragments arranged by size:
698 320 8
PmlI CAC'GTG
Cuts at: 0 72 138 1026
Size: 72 66 888
Fragments arranged by size:
888 72 66
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 485 517 1026
Size: 485 32 509
Fragments arranged by size:
509 485 32
PpuMI rG'GwC_Cy
Cuts at: 0 201 278 553 1026
Size: 201 77 275 473
Fragments arranged by size:
473 275 201 77
PspOMI G'GGCC_C
Cuts at: 0 396 1026
Size: 396 630
PstI C_TGCA'G
Cuts at: 0 439 510 1026
Size: 439 71 516
Fragments arranged by size:
516 439 71
PvuII CAG'CTG
Cuts at: 0 1018 1026
Size: 1018 8
SacI G_AGCT'C
Cuts at: 0 218 451 1026
Size: 218 233 575
Fragments arranged by size:
575 233 218
SanDI GG'GwC_CC
Cuts at: 0 201 1026
Size: 201 825
SfcI C'TryA_G
Cuts at: 0 282 435 506 576 1026
Size: 282 153 71 70 450
Fragments arranged by size:
450 282 153 71 70
SmlI C'TyrA_G
Cuts at: 0 125 1026
Size: 125 901
SspI AAT'ATT
Cuts at: 0 230 1026
Size: 230 796
StuI AGG'CCT
Cuts at: 0 946 1026
Size: 946 80
StyI C'CwwG_G
Cuts at: 0 597 1026
Size: 597 429
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 27 64 345 577 696 1026
Size: 27 37 281 232 119 330
Fragments arranged by size:
330 281 232 119 37 27
TatI w'GTAC_w
Cuts at: 0 185 1026
Size: 185 841
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 122 154 292 324 407 439 1026
Size: 122 32 138 32 83 32 587
Fragments arranged by size:
587 138 122 83 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 429 1026
Size: 429 597
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 339 1026
Size: 339 687
XmnI GAAnn'nnTTC
Cuts at: 0 241 660 1026
Size: 241 419 366
Fragments arranged by size:
419 366 241
Enzymes that do cut:
AfeI AflIII AleI AloI AlwNI ApaI BaeI BanI
BanII BclI BlpI Bme1580I Bpu10I BpuEI BsaI BsaAI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrGI BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI
EcoICRI EcoNI EcoO109I HaeII HindIII MmeI MslI MspA1I
NcoI NspI PflMI PmlI PpiI PpuMI PspOMI PstI
PvuII SacI SanDI SfcI SmlI SspI StuI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AlfI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BmgBI BmrI BmtI BplI BpmI BsaBI
BsaHI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EciI
Eco57MI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI XbaI XhoI XmaI ZraI