Restriction map for
pF1KB6550
(Linear) (Six-base) MAPSORT of: KIBB6550.seq Check: 291 from: 1 to: 1176
KIBB6550.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 455 1172 1176
Size: 371 84 717 4
Fragments arranged by size:
717 371 84 4
AflIII A'CryG_T
Cuts at: 0 372 1176
Size: 372 804
ApoI r'AATT_y
Cuts at: 0 85 522 1176
Size: 85 437 654
Fragments arranged by size:
654 437 85
AvaI C'yCGr_G
Cuts at: 0 53 887 1176
Size: 53 834 289
Fragments arranged by size:
834 289 53
BanI G'GyrC_C
Cuts at: 0 46 1176
Size: 46 1130
BanII G_rGCy'C
Cuts at: 0 1150 1176
Size: 1150 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 1142 1176
Size: 1142 34
BbvCI CC'TCA_GC
Cuts at: 0 772 1176
Size: 772 404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 541 575 1176
Size: 541 34 601
Fragments arranged by size:
601 541 34
BclI T'GATC_A
Cuts at: 0 621 1176
Size: 621 555
Bme1580I G_kGCm'C
Cuts at: 0 49 1176
Size: 49 1127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 890 1176
Size: 890 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 903 1176
Size: 903 273
Bpu10I CC'TnA_GC
Cuts at: 0 772 1176
Size: 772 404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 1176
Size: 317 859
BsaI GGTCTCn'nnnn_
Cuts at: 0 1016 1176
Size: 1016 160
BsaAI yAC'GTr
Cuts at: 0 856 1176
Size: 856 320
BsaBI GATnn'nnATC
Cuts at: 0 290 882 1176
Size: 290 592 294
Fragments arranged by size:
592 294 290
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 789 882 1011 1158 1176
Size: 789 93 129 147 18
Fragments arranged by size:
789 147 129 93 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1176
Size: 625 551
BsiHKAI G_wGCw'C
Cuts at: 0 1150 1176
Size: 1150 26
Bsp1286I G_dGCh'C
Cuts at: 0 49 1150 1176
Size: 49 1101 26
Fragments arranged by size:
1101 49 26
BspHI T'CATG_A
Cuts at: 0 135 378 597 1176
Size: 135 243 219 579
Fragments arranged by size:
579 243 219 135
BsrBI CCG'CTC
Cuts at: 0 205 917 1176
Size: 205 712 259
Fragments arranged by size:
712 259 205
BsrDI GCAATG_nn'
Cuts at: 0 278 1176
Size: 278 898
BssHII G'CGCG_C
Cuts at: 0 21 1176
Size: 21 1155
BssSI C'ACGA_G
Cuts at: 0 1076 1176
Size: 1076 100
BstYI r'GATC_y
Cuts at: 0 162 808 1000 1176
Size: 162 646 192 176
Fragments arranged by size:
646 192 176 162
BtgI C'CryG_G
Cuts at: 0 257 1176
Size: 257 919
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 40 811 886 1176
Size: 40 771 75 290
Fragments arranged by size:
771 290 75 40
ClaI AT'CG_AT
Cuts at: 0 837 1176
Size: 837 339
DraIII CAC_nnn'GTG
Cuts at: 0 1078 1176
Size: 1078 98
EaeI y'GGCC_r
Cuts at: 0 308 1176
Size: 308 868
EarI CTCTTCn'nnn_
Cuts at: 0 332 732 737 1148 1176
Size: 332 400 5 411 28
Fragments arranged by size:
411 400 332 28 5
EcoICRI GAG'CTC
Cuts at: 0 1148 1176
Size: 1148 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 455 903 1172 1176
Size: 371 84 448 269 4
Fragments arranged by size:
448 371 269 84 4
EcoO109I rG'GnC_Cy
Cuts at: 0 779 911 1176
Size: 779 132 265
Fragments arranged by size:
779 265 132
EcoRI G'AATT_C
Cuts at: 0 522 1176
Size: 522 654
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1176
Size: 1021 32 123
Fragments arranged by size:
1021 123 32
HincII GTy'rAC
Cuts at: 0 76 1176
Size: 76 1100
HindIII A'AGCT_T
Cuts at: 0 670 1176
Size: 670 506
MscI TGG'CCA
Cuts at: 0 310 1176
Size: 310 866
MslI CAynn'nnrTG
Cuts at: 0 602 1176
Size: 602 574
NcoI C'CATG_G
Cuts at: 0 257 1176
Size: 257 919
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 192 1176
Size: 42 150 984
Fragments arranged by size:
984 150 42
PmlI CAC'GTG
Cuts at: 0 856 1176
Size: 856 320
PpuMI rG'GwC_Cy
Cuts at: 0 779 911 1176
Size: 779 132 265
Fragments arranged by size:
779 265 132
PstI C_TGCA'G
Cuts at: 0 272 1176
Size: 272 904
SacI G_AGCT'C
Cuts at: 0 1150 1176
Size: 1150 26
SapI GCTCTTCn'nnn_
Cuts at: 0 1148 1176
Size: 1148 28
SfcI C'TryA_G
Cuts at: 0 268 1176
Size: 268 908
SmaI CCC'GGG
Cuts at: 0 55 1176
Size: 55 1121
SmlI C'TyrA_G
Cuts at: 0 332 1176
Size: 332 844
StyI C'CwwG_G
Cuts at: 0 257 413 1176
Size: 257 156 763
Fragments arranged by size:
763 257 156
XbaI T'CTAG_A
Cuts at: 0 967 1176
Size: 967 209
XmaI C'CCGG_G
Cuts at: 0 53 1176
Size: 53 1123
XmnI GAAnn'nnTTC
Cuts at: 0 374 1176
Size: 374 802
Enzymes that do cut:
AcuI AflIII ApoI AvaI BanI BanII BbsI BbvCI
BcgI BclI Bme1580I BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BseRI BsgI BsiHKAI Bsp1286I BspHI BsrBI
BsrDI BssHII BssSI BstYI BtgI BtgZI ClaI DraIII
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII
HindIII MscI MslI NcoI PflMI PmlI PpuMI PstI
SacI SapI SfcI SmaI SmlI StyI XbaI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrFI BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtsI CspCI DraI DrdI EagI EciI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XcmI XhoI ZraI