Restriction map for
pF1KB6547
(Linear) (Six-base) MAPSORT of: KIBB6547.seq Check: 8721 from: 1 to: 1110
KIBB6547.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1110
Size: 871 239
AflII C'TTAA_G
Cuts at: 0 988 1110
Size: 988 122
AflIII A'CryG_T
Cuts at: 0 435 1110
Size: 435 675
ApaLI G'TGCA_C
Cuts at: 0 146 1110
Size: 146 964
ApoI r'AATT_y
Cuts at: 0 138 235 304 318 653 1110
Size: 138 97 69 14 335 457
Fragments arranged by size:
457 335 138 97 69 14
AseI AT'TA_AT
Cuts at: 0 661 1110
Size: 661 449
AvaI C'yCGr_G
Cuts at: 0 47 1110
Size: 47 1063
BanII G_rGCy'C
Cuts at: 0 942 1110
Size: 942 168
BbsI GAAGACnn'nnnn_
Cuts at: 0 45 677 1110
Size: 45 632 433
Fragments arranged by size:
632 433 45
BclI T'GATC_A
Cuts at: 0 969 1110
Size: 969 141
Bme1580I G_kGCm'C
Cuts at: 0 150 1110
Size: 150 960
BmgBI CAC'GTC
Cuts at: 0 63 1110
Size: 63 1047
BmrI ACTGGGnnnn_n'
Cuts at: 0 32 194 723 1110
Size: 32 162 529 387
Fragments arranged by size:
529 387 162 32
Bpu10I CC'TnA_GC
Cuts at: 0 998 1110
Size: 998 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1110
Size: 926 184
BsaAI yAC'GTr
Cuts at: 0 756 1110
Size: 756 354
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 407 1110
Size: 407 703
BsiHKAI G_wGCw'C
Cuts at: 0 150 942 1110
Size: 150 792 168
Fragments arranged by size:
792 168 150
Bsp1286I G_dGCh'C
Cuts at: 0 150 942 1110
Size: 150 792 168
Fragments arranged by size:
792 168 150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 522 1110
Size: 522 588
BsrGI T'GTAC_A
Cuts at: 0 154 1110
Size: 154 956
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1093 1110
Size: 1093 17
Bsu36I CC'TnA_GG
Cuts at: 0 617 1110
Size: 617 493
DraI TTT'AAA
Cuts at: 0 638 1110
Size: 638 472
EaeI y'GGCC_r
Cuts at: 0 447 1110
Size: 447 663
EcoICRI GAG'CTC
Cuts at: 0 940 1110
Size: 940 170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1110
Size: 871 239
EcoRV GAT'ATC
Cuts at: 0 117 1110
Size: 117 993
MfeI C'AATT_G
Cuts at: 0 875 1110
Size: 875 235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 436 1110
Size: 56 380 674
Fragments arranged by size:
674 380 56
MscI TGG'CCA
Cuts at: 0 449 1110
Size: 449 661
MslI CAynn'nnrTG
Cuts at: 0 425 1110
Size: 425 685
NspI r_CATG'y
Cuts at: 0 439 1110
Size: 439 671
PciI A'CATG_T
Cuts at: 0 435 1110
Size: 435 675
PflMI CCAn_nnn'nTGG
Cuts at: 0 586 1110
Size: 586 524
PspXI vC'TCGA_Gb
Cuts at: 0 47 1110
Size: 47 1063
SacI G_AGCT'C
Cuts at: 0 942 1110
Size: 942 168
SfcI C'TryA_G
Cuts at: 0 809 1110
Size: 809 301
SmlI C'TyrA_G
Cuts at: 0 47 941 988 1110
Size: 47 894 47 122
Fragments arranged by size:
894 122 47 47
SspI AAT'ATT
Cuts at: 0 575 834 1110
Size: 575 259 276
Fragments arranged by size:
575 276 259
TatI w'GTAC_w
Cuts at: 0 154 825 1110
Size: 154 671 285
Fragments arranged by size:
671 285 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 393 425 455 487 1110
Size: 393 32 30 32 623
Fragments arranged by size:
623 393 32 32 30
XhoI C'TCGA_G
Cuts at: 0 47 1110
Size: 47 1063
XmnI GAAnn'nnTTC
Cuts at: 0 140 1110
Size: 140 970
Enzymes that do cut:
AcuI AflII AflIII ApaLI ApoI AseI AvaI BanII
BbsI BclI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaAI
BseRI BsiHKAI Bsp1286I BspMI BsrGI BstAPI Bsu36I DraI
EaeI EcoICRI Eco57MI EcoRV MfeI MmeI MscI MslI
NspI PciI PflMI PspXI SacI SfcI SmlI SspI
TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI BmtI BplI BpmI BsaI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EarI EciI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
StyI SwaI TaqII Tth111I XbaI XcmI XmaI ZraI