(Linear) (Six-base) MAPSORT of: KIBB6545.seq  Check: 7151  from: 1  to: 1095



KIBB6545.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94    1095 
   Size:         94    1001
 
ApoI r'AATT_y

Cuts at:      0     655     781    1095 
   Size:        655     126     314

  Fragments arranged by size: 

                 655     314     126
 
AseI AT'TA_AT

Cuts at:      0     557     940    1095 
   Size:        557     383     155

  Fragments arranged by size: 

                 557     383     155
 
AvaI C'yCGr_G

Cuts at:      0      96    1095 
   Size:         96     999
 
BamHI G'GATC_C

Cuts at:      0     964    1095 
   Size:        964     131
 
BanI G'GyrC_C

Cuts at:      0     712    1095 
   Size:        712     383
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     265     887    1095 
   Size:        265     622     208

  Fragments arranged by size: 

                 622     265     208
 
BclI T'GATC_A

Cuts at:      0     680    1095 
   Size:        680     415
 
BlpI GC'TnA_GC

Cuts at:      0     290    1095 
   Size:        290     805
 
BmgBI CAC'GTC

Cuts at:      0     908    1095 
   Size:        908     187
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     885    1095 
   Size:        885     210
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     231    1095 
   Size:        231     864
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     447     875    1093    1095 
   Size:        340     107     428     218       2

  Fragments arranged by size: 

                 428     340     218     107       2
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     288    1095 
   Size:         25     263     807

  Fragments arranged by size: 

                 807     263      25
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     465     760    1095 
   Size:        465     295     335

  Fragments arranged by size: 

                 465     335     295
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     745     775    1095 
   Size:        745      30     320

  Fragments arranged by size: 

                 745     320      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19      28     559     753    1095 
   Size:         19       9     531     194     342

  Fragments arranged by size: 

                 531     342     194      19       9
 
BseYI C'CCAG_C

Cuts at:      0       3     602    1095 
   Size:          3     599     493

  Fragments arranged by size: 

                 599     493       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     868    1095 
   Size:        868     227
 
BsmI GAATG_Cn'

Cuts at:      0     648    1095 
   Size:        648     447
 
Bsp1286I G_dGCh'C

Cuts at:      0     868    1095 
   Size:        868     227
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     586     927    1095 
   Size:        586     341     168

  Fragments arranged by size: 

                 586     341     168
 
BsrDI GCAATG_nn'

Cuts at:      0     149     480     588    1095 
   Size:        149     331     108     507

  Fragments arranged by size: 

                 507     331     149     108
 
BssSI C'ACGA_G

Cuts at:      0     832    1095 
   Size:        832     263
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      62    1095 
   Size:         62    1033
 
BstEII G'GTnAC_C

Cuts at:      0      15     324    1095 
   Size:         15     309     771

  Fragments arranged by size: 

                 771     309      15
 
BstYI r'GATC_y

Cuts at:      0     416     810     964    1095 
   Size:        416     394     154     131

  Fragments arranged by size: 

                 416     394     154     131
 
EaeI y'GGCC_r

Cuts at:      0     667    1095 
   Size:        667     428
 
EarI CTCTTCn'nnn_

Cuts at:      0     113     967    1095 
   Size:        113     854     128

  Fragments arranged by size: 

                 854     128     113
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     340     447     875    1093    1095 
   Size:         94     246     107     428     218       2

  Fragments arranged by size: 

                 428     246     218     107      94       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     541    1095 
   Size:        541     554
 
EcoRI G'AATT_C

Cuts at:      0     655    1095 
   Size:        655     440
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     745     777    1095 
   Size:        745      32     318

  Fragments arranged by size: 

                 745     318      32
 
HincII GTy'rAC

Cuts at:      0     144     552    1030    1095 
   Size:        144     408     478      65

  Fragments arranged by size: 

                 478     408     144      65
 
MspA1I CmG'CkG

Cuts at:      0      76    1095 
   Size:         76    1019
 
PfoI T'CCnGG_A

Cuts at:      0     419     852     952     967    1095 
   Size:        419     433     100      15     128

  Fragments arranged by size: 

                 433     419     128     100      15
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     517     549    1095 
   Size:        517      32     546

  Fragments arranged by size: 

                 546     517      32
 
PvuII CAG'CTG

Cuts at:      0      76    1095 
   Size:         76    1019
 
SfcI C'TryA_G

Cuts at:      0     400    1095 
   Size:        400     695
 
SmlI C'TyrA_G

Cuts at:      0      40     267    1095 
   Size:         40     227     828

  Fragments arranged by size: 

                 828     227      40
 
StyI C'CwwG_G

Cuts at:      0     708     776    1095 
   Size:        708      68     319

  Fragments arranged by size: 

                 708     319      68
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     973    1005    1095 
   Size:        973      32      90

  Fragments arranged by size: 

                 973      90      32
 

 Enzymes that do cut:

     AcuI     ApoI     AseI     AvaI    BamHI     BanI     BbsI     BclI
     BlpI    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaXI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BssSI
   BstAPI   BstEII    BstYI     EaeI     EarI  Eco57MI    EcoNI    EcoRI
    Hin4I   HincII   MspA1I     PfoI     PsrI    PvuII     SfcI     SmlI
     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII Bme1580I     BmtI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BstBI    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI