Restriction map for
pF1KB6539
(Linear) (Six-base) MAPSORT of: KIBB6539.seq Check: 7837 from: 1 to: 1104
KIBB6539.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 776 1104
Size: 776 328
ApaI G_GGCC'C
Cuts at: 0 474 615 801 1104
Size: 474 141 186 303
Fragments arranged by size:
474 303 186 141
BanI G'GyrC_C
Cuts at: 0 211 409 1104
Size: 211 198 695
Fragments arranged by size:
695 211 198
BanII G_rGCy'C
Cuts at: 0 336 474 511 615 801 1063 1104
Size: 336 138 37 104 186 262 41
Fragments arranged by size:
336 262 186 138 104 41 37
BciVI GTATCCnnnnn_n'
Cuts at: 0 156 1104
Size: 156 948
Bme1580I G_kGCm'C
Cuts at: 0 474 615 801 1104
Size: 474 141 186 303
Fragments arranged by size:
474 303 186 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 786 1104
Size: 786 318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 1104
Size: 414 690
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 867 1104
Size: 867 237
BsaAI yAC'GTr
Cuts at: 0 893 1104
Size: 893 211
BsaBI GATnn'nnATC
Cuts at: 0 283 1104
Size: 283 821
BsaHI Gr'CG_yC
Cuts at: 0 191 1104
Size: 191 913
BsaWI w'CCGG_w
Cuts at: 0 377 1104
Size: 377 727
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 438 739 742 763 1104
Size: 438 301 3 21 341
Fragments arranged by size:
438 341 301 21 3
BseYI C'CCAG_C
Cuts at: 0 467 608 1104
Size: 467 141 496
Fragments arranged by size:
496 467 141
BsiHKAI G_wGCw'C
Cuts at: 0 370 511 1104
Size: 370 141 593
Fragments arranged by size:
593 370 141
Bsp1286I G_dGCh'C
Cuts at: 0 336 370 474 511 615 801 1063 1104
Size: 336 34 104 37 104 186 262 41
Fragments arranged by size:
336 262 186 104 104 41 37 34
BspHI T'CATG_A
Cuts at: 0 303 1104
Size: 303 801
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 235 1104
Size: 235 869
BsrDI GCAATG_nn'
Cuts at: 0 273 603 1104
Size: 273 330 501
Fragments arranged by size:
501 330 273
BssSI C'ACGA_G
Cuts at: 0 427 1104
Size: 427 677
BstEII G'GTnAC_C
Cuts at: 0 993 1104
Size: 993 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 966 1104
Size: 966 138
BstYI r'GATC_y
Cuts at: 0 278 1104
Size: 278 826
Bsu36I CC'TnA_GG
Cuts at: 0 295 1104
Size: 295 809
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 813 1104
Size: 813 291
BtsI GCAGTG_nn'
Cuts at: 0 1030 1104
Size: 1030 74
DrdI GACnn_nn'nnGTC
Cuts at: 0 244 382 1104
Size: 244 138 722
Fragments arranged by size:
722 244 138
EaeI y'GGCC_r
Cuts at: 0 735 1104
Size: 735 369
EarI CTCTTCn'nnn_
Cuts at: 0 62 415 1104
Size: 62 353 689
Fragments arranged by size:
689 353 62
EcoICRI GAG'CTC
Cuts at: 0 509 1104
Size: 509 595
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 1104
Size: 414 690
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1003 1104
Size: 1003 101
EcoO109I rG'GnC_Cy
Cuts at: 0 310 471 612 798 1104
Size: 310 161 141 186 306
Fragments arranged by size:
310 306 186 161 141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 109 1104
Size: 109 995
FspI TGC'GCA
Cuts at: 0 265 1104
Size: 265 839
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 263 295 634 666 865 897 1104
Size: 263 32 339 32 199 32 207
Fragments arranged by size:
339 263 207 199 32 32 32
HincII GTy'rAC
Cuts at: 0 1047 1104
Size: 1047 57
MslI CAynn'nnrTG
Cuts at: 0 431 1104
Size: 431 673
MspA1I CmG'CkG
Cuts at: 0 608 836 1104
Size: 608 228 268
Fragments arranged by size:
608 268 228
NspI r_CATG'y
Cuts at: 0 436 940 1104
Size: 436 504 164
Fragments arranged by size:
504 436 164
PpuMI rG'GwC_Cy
Cuts at: 0 310 1104
Size: 310 794
PspOMI G'GGCC_C
Cuts at: 0 470 611 797 1104
Size: 470 141 186 307
Fragments arranged by size:
470 307 186 141
PvuII CAG'CTG
Cuts at: 0 608 836 1104
Size: 608 228 268
Fragments arranged by size:
608 268 228
SacI G_AGCT'C
Cuts at: 0 511 1104
Size: 511 593
SapI GCTCTTCn'nnn_
Cuts at: 0 415 1104
Size: 415 689
SfcI C'TryA_G
Cuts at: 0 102 1104
Size: 102 1002
SmlI C'TyrA_G
Cuts at: 0 882 1104
Size: 882 222
SphI G_CATG'C
Cuts at: 0 436 1104
Size: 436 668
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 662 1017 1104
Size: 662 355 87
Fragments arranged by size:
662 355 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 724 756 1104
Size: 724 32 348
Fragments arranged by size:
724 348 32
Enzymes that do cut:
AlwNI ApaI BanI BanII BciVI Bme1580I BmrI BpmI
BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BssSI BstEII BstXI BstYI
Bsu36I BtgZI BtsI DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI Hin4I HincII MslI MspA1I
NspI PpuMI PspOMI PvuII SacI SapI SfcI SmlI
SphI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstZ17I BtgI ClaI CspCI DraI DraIII
EagI EciI EcoRI EcoRV FseI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI
SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI