Restriction map for
pF1KB6533
(Linear) (Six-base) MAPSORT of: KIBB6533.seq Check: 7479 from: 1 to: 1101
KIBB6533.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 418 1101
Size: 418 683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 879 1054 1101
Size: 126 753 175 47
Fragments arranged by size:
753 175 126 47
AleI CACnn'nnGTG
Cuts at: 0 151 1009 1101
Size: 151 858 92
Fragments arranged by size:
858 151 92
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 169 595 1101
Size: 169 426 506
Fragments arranged by size:
506 426 169
AlwNI CAG_nnn'CTG
Cuts at: 0 488 704 915 936 961 1101
Size: 488 216 211 21 25 140
Fragments arranged by size:
488 216 211 140 25 21
BanI G'GyrC_C
Cuts at: 0 16 158 172 232 283 298 994 1101
Size: 16 142 14 60 51 15 696 107
Fragments arranged by size:
696 142 107 60 51 16 15 14
BanII G_rGCy'C
Cuts at: 0 212 434 970 1078 1101
Size: 212 222 536 108 23
Fragments arranged by size:
536 222 212 108 23
BbeI G_GCGC'C
Cuts at: 0 162 176 236 287 1101
Size: 162 14 60 51 814
Fragments arranged by size:
814 162 60 51 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 345 1101
Size: 345 756
BciVI GTATCCnnnnn_n'
Cuts at: 0 364 1101
Size: 364 737
BclI T'GATC_A
Cuts at: 0 608 1101
Size: 608 493
BglI GCCn_nnn'nGGC
Cuts at: 0 313 779 1101
Size: 313 466 322
Fragments arranged by size:
466 322 313
BlpI GC'TnA_GC
Cuts at: 0 433 1060 1101
Size: 433 627 41
Fragments arranged by size:
627 433 41
Bme1580I G_kGCm'C
Cuts at: 0 19 200 1101
Size: 19 181 901
Fragments arranged by size:
901 181 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 205 770 1101
Size: 50 155 565 331
Fragments arranged by size:
565 331 155 50
BsaHI Gr'CG_yC
Cuts at: 0 159 173 233 284 1101
Size: 159 14 60 51 817
Fragments arranged by size:
817 159 60 51 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 685 715 743 773 1074 1101
Size: 73 30 582 30 28 30 301 27
Fragments arranged by size:
582 301 73 30 30 30 28 27
BseYI C'CCAG_C
Cuts at: 0 48 961 1093 1101
Size: 48 913 132 8
Fragments arranged by size:
913 132 48 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 501 1101
Size: 426 75 600
Fragments arranged by size:
600 426 75
BsiEI CG_ry'CG
Cuts at: 0 219 317 990 1101
Size: 219 98 673 111
Fragments arranged by size:
673 219 111 98
BsiHKAI G_wGCw'C
Cuts at: 0 191 212 986 1101
Size: 191 21 774 115
Fragments arranged by size:
774 191 115 21
BsmI GAATG_Cn'
Cuts at: 0 750 1101
Size: 750 351
BsmBI CGTCTCn'nnnn_
Cuts at: 0 621 1032 1101
Size: 621 411 69
Fragments arranged by size:
621 411 69
Bsp1286I G_dGCh'C
Cuts at: 0 19 191 200 212 434 970 986 1078
Size: 19 172 9 12 222 536 16 92
Cuts at: 1078 1101
Size: 23
Fragments arranged by size:
536 222 172 92 23 19 16 12
9
BsrBI CCG'CTC
Cuts at: 0 61 89 1101
Size: 61 28 1012
Fragments arranged by size:
1012 61 28
BsrDI GCAATG_nn'
Cuts at: 0 73 1101
Size: 73 1028
BsrFI r'CCGG_y
Cuts at: 0 109 121 229 316 1101
Size: 109 12 108 87 785
Fragments arranged by size:
785 109 108 87 12
BssHII G'CGCG_C
Cuts at: 0 320 337 339 1101
Size: 320 17 2 762
Fragments arranged by size:
762 320 17 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 195 488 1101
Size: 72 123 293 613
Fragments arranged by size:
613 293 123 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 183 1101
Size: 183 918
BtgI C'CryG_G
Cuts at: 0 176 213 245 425 974 1101
Size: 176 37 32 180 549 127
Fragments arranged by size:
549 180 176 127 37 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 102 1101
Size: 102 999
BtsI GCAGTG_nn'
Cuts at: 0 558 768 1101
Size: 558 210 333
Fragments arranged by size:
558 333 210
DraIII CAC_nnn'GTG
Cuts at: 0 195 246 1101
Size: 195 51 855
Fragments arranged by size:
855 195 51
EaeI y'GGCC_r
Cuts at: 0 111 216 227 314 1101
Size: 111 105 11 87 787
Fragments arranged by size:
787 111 105 87 11
EagI C'GGCC_G
Cuts at: 0 216 314 1101
Size: 216 98 787
Fragments arranged by size:
787 216 98
EcoICRI GAG'CTC
Cuts at: 0 210 1101
Size: 210 891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 126 205 770 879 1054 1101
Size: 50 76 79 565 109 175 47
Fragments arranged by size:
565 175 109 79 76 50 47
HaeII r_GCGC'y
Cuts at: 0 46 162 176 236 272 287 1101
Size: 46 116 14 60 36 15 814
Fragments arranged by size:
814 116 60 46 36 15 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 685 717 813 845 1101
Size: 73 32 580 32 96 32 256
Fragments arranged by size:
580 256 96 73 32 32 32
HincII GTy'rAC
Cuts at: 0 132 419 1101
Size: 132 287 682
Fragments arranged by size:
682 287 132
KasI G'GCGC_C
Cuts at: 0 158 172 232 283 1101
Size: 158 14 60 51 818
Fragments arranged by size:
818 158 60 51 14
MfeI C'AATT_G
Cuts at: 0 624 1101
Size: 624 477
MslI CAynn'nnrTG
Cuts at: 0 10 151 670 979 1009 1101
Size: 10 141 519 309 30 92
Fragments arranged by size:
519 309 141 92 30 10
MspA1I CmG'CkG
Cuts at: 0 215 912 1097 1101
Size: 215 697 185 4
Fragments arranged by size:
697 215 185 4
NaeI GCC'GGC
Cuts at: 0 111 123 231 318 1101
Size: 111 12 108 87 783
Fragments arranged by size:
783 111 108 87 12
NarI GG'CG_CC
Cuts at: 0 159 173 233 284 1101
Size: 159 14 60 51 817
Fragments arranged by size:
817 159 60 51 14
NcoI C'CATG_G
Cuts at: 0 176 425 974 1101
Size: 176 249 549 127
Fragments arranged by size:
549 249 176 127
NgoMIV G'CCGG_C
Cuts at: 0 109 121 229 316 1101
Size: 109 12 108 87 785
Fragments arranged by size:
785 109 108 87 12
NotI GC'GGCC_GC
Cuts at: 0 216 1101
Size: 216 885
NspI r_CATG'y
Cuts at: 0 582 1101
Size: 582 519
PflMI CCAn_nnn'nTGG
Cuts at: 0 246 936 1101
Size: 246 690 165
Fragments arranged by size:
690 246 165
PstI C_TGCA'G
Cuts at: 0 493 920 1101
Size: 493 427 181
Fragments arranged by size:
493 427 181
PvuII CAG'CTG
Cuts at: 0 912 1097 1101
Size: 912 185 4
Fragments arranged by size:
912 185 4
SacI G_AGCT'C
Cuts at: 0 212 1101
Size: 212 889
SacII CC_GC'GG
Cuts at: 0 216 1101
Size: 216 885
SalI G'TCGA_C
Cuts at: 0 417 1101
Size: 417 684
ScaI AGT'ACT
Cuts at: 0 450 1101
Size: 450 651
SfcI C'TryA_G
Cuts at: 0 489 916 1101
Size: 489 427 185
Fragments arranged by size:
489 427 185
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 313 1101
Size: 313 788
SfoI GGC'GCC
Cuts at: 0 160 174 234 285 1101
Size: 160 14 60 51 816
Fragments arranged by size:
816 160 60 51 14
StuI AGG'CCT
Cuts at: 0 35 850 1101
Size: 35 815 251
Fragments arranged by size:
815 251 35
StyI C'CwwG_G
Cuts at: 0 167 176 425 590 974 1101
Size: 167 9 249 165 384 127
Fragments arranged by size:
384 249 167 165 127 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 467 1004 1101
Size: 467 537 97
Fragments arranged by size:
537 467 97
TatI w'GTAC_w
Cuts at: 0 448 656 1101
Size: 448 208 445
Fragments arranged by size:
448 445 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 463 495 743 775 1101
Size: 463 32 248 32 326
Fragments arranged by size:
463 326 248 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 1101
Size: 174 927
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI BanI BanII BbeI
BbsI BciVI BclI BglI BlpI Bme1580I BpmI BsaHI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BsrBI BsrDI BsrFI BssHII BstAPI BstXI BtgI BtgZI
BtsI DraIII EaeI EagI EcoICRI Eco57MI HaeII Hin4I
HincII KasI MfeI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PflMI PstI PvuII SacI SacII
SalI ScaI SfcI SfiI SfoI StuI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BfrBI BglII
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BseRI BsiWI BspEI BspHI BspMI BsrGI
BssSI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EarI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MluI
MmeI MscI NdeI NheI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SanDI SapI SbfI
SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI