Restriction map for
pF1KB6532
(Linear) (Six-base) MAPSORT of: KIBB6532.seq Check: 3396 from: 1 to: 1167
KIBB6532.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 252 611 1167
Size: 252 359 556
Fragments arranged by size:
556 359 252
AclI AA'CG_TT
Cuts at: 0 766 1167
Size: 766 401
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 160 215 295 725 1064 1167
Size: 10 150 55 80 430 339 103
Fragments arranged by size:
430 339 150 103 80 55 10
AflII C'TTAA_G
Cuts at: 0 76 1167
Size: 76 1091
AflIII A'CryG_T
Cuts at: 0 362 1167
Size: 362 805
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 61 1167
Size: 61 1106
ApoI r'AATT_y
Cuts at: 0 202 1167
Size: 202 965
BamHI G'GATC_C
Cuts at: 0 345 855 1167
Size: 345 510 312
Fragments arranged by size:
510 345 312
BbsI GAAGACnn'nnnn_
Cuts at: 0 1100 1167
Size: 1100 67
BclI T'GATC_A
Cuts at: 0 641 824 1071 1167
Size: 641 183 247 96
Fragments arranged by size:
641 247 183 96
BfrBI ATG'CAT
Cuts at: 0 683 1167
Size: 683 484
BglII A'GATC_T
Cuts at: 0 1023 1167
Size: 1023 144
BmtI G_CTAG'C
Cuts at: 0 566 1167
Size: 566 601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1167
Size: 1159 8
BsiEI CG_ry'CG
Cuts at: 0 72 1167
Size: 72 1095
BsiHKAI G_wGCw'C
Cuts at: 0 636 1167
Size: 636 531
Bsp1286I G_dGCh'C
Cuts at: 0 636 1167
Size: 636 531
BspHI T'CATG_A
Cuts at: 0 320 369 1074 1100 1167
Size: 320 49 705 26 67
Fragments arranged by size:
705 320 67 49 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 940 1039 1167
Size: 940 99 128
Fragments arranged by size:
940 128 99
BssSI C'ACGA_G
Cuts at: 0 551 1167
Size: 551 616
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1066 1167
Size: 1066 101
BstYI r'GATC_y
Cuts at: 0 345 422 855 1023 1167
Size: 345 77 433 168 144
Fragments arranged by size:
433 345 168 144 77
EaeI y'GGCC_r
Cuts at: 0 69 1167
Size: 69 1098
EagI C'GGCC_G
Cuts at: 0 69 1167
Size: 69 1098
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1167
Size: 19 1148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 160 215 295 725 1064 1159 1167
Size: 10 150 55 80 430 339 95 8
Fragments arranged by size:
430 339 150 95 80 55 10 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 500 1167
Size: 500 667
EcoO109I rG'GnC_Cy
Cuts at: 0 144 375 952 1167
Size: 144 231 577 215
Fragments arranged by size:
577 231 215 144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 311 1167
Size: 311 856
HaeII r_GCGC'y
Cuts at: 0 25 1167
Size: 25 1142
HincII GTy'rAC
Cuts at: 0 191 253 1167
Size: 191 62 914
Fragments arranged by size:
914 191 62
HindIII A'AGCT_T
Cuts at: 0 997 1167
Size: 997 170
HpaI GTT'AAC
Cuts at: 0 191 1167
Size: 191 976
MfeI C'AATT_G
Cuts at: 0 989 1167
Size: 989 178
MslI CAynn'nnrTG
Cuts at: 0 691 1167
Size: 691 476
MspA1I CmG'CkG
Cuts at: 0 570 745 1167
Size: 570 175 422
Fragments arranged by size:
570 422 175
NheI G'CTAG_C
Cuts at: 0 562 1167
Size: 562 605
NsiI A_TGCA'T
Cuts at: 0 685 1167
Size: 685 482
NspI r_CATG'y
Cuts at: 0 366 1167
Size: 366 801
PasI CC'CwG_GG
Cuts at: 0 379 1167
Size: 379 788
PciI A'CATG_T
Cuts at: 0 362 1167
Size: 362 805
PfoI T'CCnGG_A
Cuts at: 0 425 873 1167
Size: 425 448 294
Fragments arranged by size:
448 425 294
PpuMI rG'GwC_Cy
Cuts at: 0 375 952 1167
Size: 375 577 215
Fragments arranged by size:
577 375 215
PshAI GACnn'nnGTC
Cuts at: 0 71 1167
Size: 71 1096
PsiI TTA'TAA
Cuts at: 0 359 1167
Size: 359 808
PvuII CAG'CTG
Cuts at: 0 570 745 1167
Size: 570 175 422
Fragments arranged by size:
570 422 175
SalI G'TCGA_C
Cuts at: 0 251 1167
Size: 251 916
SexAI A'CCwGG_T
Cuts at: 0 525 1167
Size: 525 642
SfcI C'TryA_G
Cuts at: 0 148 1167
Size: 148 1019
SmlI C'TyrA_G
Cuts at: 0 76 1167
Size: 76 1091
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 160 1167
Size: 160 1007
TatI w'GTAC_w
Cuts at: 0 617 1039 1086 1167
Size: 617 422 47 81
Fragments arranged by size:
617 422 81 47
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AlfI ApoI BamHI
BbsI BclI BfrBI BglII BmtI BpmI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BssSI BstAPI BstYI EaeI EagI
EciI Eco57MI EcoNI EcoO109I FalI HaeII HincII HindIII
HpaI MfeI MslI MspA1I NheI NsiI NspI PasI
PciI PfoI PpuMI PshAI PsiI PvuII SalI SexAI
SfcI SmlI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AhdI AleI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanI BanII BbeI BbvCI BcgI BciVI BglI
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI
EcoRI EcoRV FseI FspI FspAI Hin4I KasI KpnI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NotI NruI PacI PflMI PmeI PmlI PpiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI