Restriction map for
pF1KB6524
(Linear) (Six-base) MAPSORT of: KIBB6524.seq Check: 7873 from: 1 to: 1161
KIBB6524.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 465 1161
Size: 465 696
AccI GT'mk_AC
Cuts at: 0 587 1161
Size: 587 574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 649 979 1161
Size: 493 156 330 182
Fragments arranged by size:
493 330 182 156
AleI CACnn'nnGTG
Cuts at: 0 1100 1161
Size: 1100 61
AlwNI CAG_nnn'CTG
Cuts at: 0 357 1161
Size: 357 804
ApaLI G'TGCA_C
Cuts at: 0 109 1093 1161
Size: 109 984 68
Fragments arranged by size:
984 109 68
BamHI G'GATC_C
Cuts at: 0 800 1161
Size: 800 361
BanI G'GyrC_C
Cuts at: 0 708 780 885 1056 1137 1161
Size: 708 72 105 171 81 24
Fragments arranged by size:
708 171 105 81 72 24
BanII G_rGCy'C
Cuts at: 0 299 500 1161
Size: 299 201 661
Fragments arranged by size:
661 299 201
BglI GCCn_nnn'nGGC
Cuts at: 0 222 1161
Size: 222 939
Bme1580I G_kGCm'C
Cuts at: 0 113 669 785 890 1061 1097 1142 1161
Size: 113 556 116 105 171 36 45 19
Fragments arranged by size:
556 171 116 113 105 45 36 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 283 385 826 1161
Size: 181 102 102 441 335
Fragments arranged by size:
441 335 181 102 102
Bpu10I CC'TnA_GC
Cuts at: 0 146 1161
Size: 146 1015
BsaI GGTCTCn'nnnn_
Cuts at: 0 959 1161
Size: 959 202
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 14 19 451 1039 1161
Size: 14 5 432 588 122
Fragments arranged by size:
588 432 122 14 5
BseYI C'CCAG_C
Cuts at: 0 51 68 236 1161
Size: 51 17 168 925
Fragments arranged by size:
925 168 51 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 1161
Size: 987 174
BsiHKAI G_wGCw'C
Cuts at: 0 113 1097 1161
Size: 113 984 64
Fragments arranged by size:
984 113 64
BsmBI CGTCTCn'nnnn_
Cuts at: 0 121 1161
Size: 121 1040
Bsp1286I G_dGCh'C
Cuts at: 0 113 299 500 669 785 890 1061 1097
Size: 113 186 201 169 116 105 171 36
Cuts at: 1097 1142 1161
Size: 45 19
Fragments arranged by size:
201 186 171 169 116 113 105 45
36 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 221 465 1161
Size: 221 244 696
Fragments arranged by size:
696 244 221
BsrBI CCG'CTC
Cuts at: 0 1107 1161
Size: 1107 54
BsrDI GCAATG_nn'
Cuts at: 0 741 1161
Size: 741 420
BsrFI r'CCGG_y
Cuts at: 0 941 1161
Size: 941 220
BssSI C'ACGA_G
Cuts at: 0 31 1161
Size: 31 1130
BstEII G'GTnAC_C
Cuts at: 0 873 1161
Size: 873 288
BstXI CCAn_nnnn'nTGG
Cuts at: 0 256 1161
Size: 256 905
BstYI r'GATC_y
Cuts at: 0 800 1161
Size: 800 361
BstZ17I GTA'TAC
Cuts at: 0 588 1161
Size: 588 573
BtgI C'CryG_G
Cuts at: 0 540 1011 1161
Size: 540 471 150
Fragments arranged by size:
540 471 150
BtsI GCAGTG_nn'
Cuts at: 0 999 1161
Size: 999 162
EaeI y'GGCC_r
Cuts at: 0 1043 1063 1161
Size: 1043 20 98
Fragments arranged by size:
1043 98 20
EarI CTCTTCn'nnn_
Cuts at: 0 19 955 1161
Size: 19 936 206
Fragments arranged by size:
936 206 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 283 385 493 649 826 979 1161
Size: 181 102 102 108 156 177 153 182
Fragments arranged by size:
182 181 177 156 153 108 102 102
EcoO109I rG'GnC_Cy
Cuts at: 0 375 385 840 1161
Size: 375 10 455 321
Fragments arranged by size:
455 375 321 10
FspI TGC'GCA
Cuts at: 0 421 1161
Size: 421 740
HaeII r_GCGC'y
Cuts at: 0 1133 1161
Size: 1133 28
HincII GTy'rAC
Cuts at: 0 726 1161
Size: 726 435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1161
Size: 1004 157
MslI CAynn'nnrTG
Cuts at: 0 595 988 1100 1161
Size: 595 393 112 61
Fragments arranged by size:
595 393 112 61
MspA1I CmG'CkG
Cuts at: 0 169 513 662 1161
Size: 169 344 149 499
Fragments arranged by size:
499 344 169 149
NaeI GCC'GGC
Cuts at: 0 943 1161
Size: 943 218
NgoMIV G'CCGG_C
Cuts at: 0 941 1161
Size: 941 220
NspI r_CATG'y
Cuts at: 0 154 1161
Size: 154 1007
PasI CC'CwG_GG
Cuts at: 0 669 1161
Size: 669 492
PflMI CCAn_nnn'nTGG
Cuts at: 0 796 1161
Size: 796 365
PfoI T'CCnGG_A
Cuts at: 0 803 1161
Size: 803 358
PpuMI rG'GwC_Cy
Cuts at: 0 375 1161
Size: 375 786
PstI C_TGCA'G
Cuts at: 0 774 1161
Size: 774 387
PvuII CAG'CTG
Cuts at: 0 513 1161
Size: 513 648
SanDI GG'GwC_CC
Cuts at: 0 375 1161
Size: 375 786
SfcI C'TryA_G
Cuts at: 0 770 1161
Size: 770 391
StyI C'CwwG_G
Cuts at: 0 116 479 533 704 1161
Size: 116 363 54 171 457
Fragments arranged by size:
457 363 171 116 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 101 221 250 846 1161
Size: 101 120 29 596 315
Fragments arranged by size:
596 315 120 101 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 155 187 701 733 1161
Size: 155 32 514 32 428
Fragments arranged by size:
514 428 155 32 32
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaLI BamHI BanI
BanII BglI Bme1580I BpmI Bpu10I BsaI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstEII BstXI BstYI BstZ17I BtgI BtsI EaeI
EarI Eco57MI EcoO109I FspI HaeII HincII MmeI MslI
MspA1I NaeI NgoMIV NspI PasI PflMI PfoI PpuMI
PstI PvuII SanDI SfcI StyI TaqII TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BspEI BspHI BsrGI BssHII BstAPI BstBI Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII
HpaI KasI KpnI MfeI MluI MscI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI