Restriction map for
pF1KB6520
(Linear) (Six-base) MAPSORT of: KIBB6520.seq Check: 3713 from: 1 to: 1158
KIBB6520.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AatII G_ACGT'C
Cuts at: 0 890 1158
Size: 890 268
Acc65I G'GTAC_C
Cuts at: 0 272 773 1158
Size: 272 501 385
Fragments arranged by size:
501 385 272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1158
Size: 673 485
AhdI GACnn_n'nnGTC
Cuts at: 0 510 565 1158
Size: 510 55 593
Fragments arranged by size:
593 510 55
AleI CACnn'nnGTG
Cuts at: 0 752 1158
Size: 752 406
AlwNI CAG_nnn'CTG
Cuts at: 0 1001 1158
Size: 1001 157
ApaI G_GGCC'C
Cuts at: 0 836 1158
Size: 836 322
ApaLI G'TGCA_C
Cuts at: 0 400 1158
Size: 400 758
AvaI C'yCGr_G
Cuts at: 0 91 382 390 642 829 1158
Size: 91 291 8 252 187 329
Fragments arranged by size:
329 291 252 187 91 8
BamHI G'GATC_C
Cuts at: 0 61 943 1158
Size: 61 882 215
Fragments arranged by size:
882 215 61
BanI G'GyrC_C
Cuts at: 0 272 727 773 1158
Size: 272 455 46 385
Fragments arranged by size:
455 385 272 46
BanII G_rGCy'C
Cuts at: 0 38 74 836 1008 1057 1158
Size: 38 36 762 172 49 101
Fragments arranged by size:
762 172 101 49 38 36
BbeI G_GCGC'C
Cuts at: 0 731 1158
Size: 731 427
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 596 884 1158
Size: 155 441 288 274
Fragments arranged by size:
441 288 274 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 332 366 1158
Size: 332 34 792
Fragments arranged by size:
792 332 34
BglI GCCn_nnn'nGGC
Cuts at: 0 377 1158
Size: 377 781
BglII A'GATC_T
Cuts at: 0 105 1158
Size: 105 1053
Bme1580I G_kGCm'C
Cuts at: 0 404 836 1158
Size: 404 432 322
Fragments arranged by size:
432 404 322
BmgBI CAC'GTC
Cuts at: 0 48 1158
Size: 48 1110
BmrI ACTGGGnnnn_n'
Cuts at: 0 426 1011 1158
Size: 426 585 147
Fragments arranged by size:
585 426 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 925 1158
Size: 160 765 233
Fragments arranged by size:
765 233 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 475 547 1158
Size: 60 415 72 611
Fragments arranged by size:
611 415 72 60
BsaHI Gr'CG_yC
Cuts at: 0 728 887 1158
Size: 728 159 271
Fragments arranged by size:
728 271 159
BsaWI w'CCGG_w
Cuts at: 0 301 1158
Size: 301 857
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 570 712 1158
Size: 570 142 446
Fragments arranged by size:
570 446 142
BseYI C'CCAG_C
Cuts at: 0 12 37 766 981 1158
Size: 12 25 729 215 177
Fragments arranged by size:
729 215 177 25 12
BsiEI CG_ry'CG
Cuts at: 0 507 1158
Size: 507 651
BsiHKAI G_wGCw'C
Cuts at: 0 74 404 1057 1158
Size: 74 330 653 101
Fragments arranged by size:
653 330 101 74
BsmI GAATG_Cn'
Cuts at: 0 960 1158
Size: 960 198
BsmBI CGTCTCn'nnnn_
Cuts at: 0 301 1158
Size: 301 857
Bsp1286I G_dGCh'C
Cuts at: 0 38 74 404 836 1008 1057 1158
Size: 38 36 330 432 172 49 101
Fragments arranged by size:
432 330 172 101 49 38 36
BspEI T'CCGG_A
Cuts at: 0 301 1158
Size: 301 857
BsrFI r'CCGG_y
Cuts at: 0 791 1158
Size: 791 367
BstAPI GCAn_nnn'nTGC
Cuts at: 0 164 344 1158
Size: 164 180 814
Fragments arranged by size:
814 180 164
BstBI TT'CG_AA
Cuts at: 0 536 1158
Size: 536 622
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1086 1158
Size: 1086 72
BstYI r'GATC_y
Cuts at: 0 61 105 212 943 1158
Size: 61 44 107 731 215
Fragments arranged by size:
731 215 107 61 44
BtgI C'CryG_G
Cuts at: 0 936 1158
Size: 936 222
DrdI GACnn_nn'nnGTC
Cuts at: 0 886 1158
Size: 886 272
EaeI y'GGCC_r
Cuts at: 0 5 1067 1158
Size: 5 1062 91
Fragments arranged by size:
1062 91 5
EarI CTCTTCn'nnn_
Cuts at: 0 256 1158
Size: 256 902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 956 1158
Size: 956 202
EcoICRI GAG'CTC
Cuts at: 0 72 1055 1158
Size: 72 983 103
Fragments arranged by size:
983 103 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 673 925 1158
Size: 160 513 252 233
Fragments arranged by size:
513 252 233 160
EcoO109I rG'GnC_Cy
Cuts at: 0 386 706 833 1158
Size: 386 320 127 325
Fragments arranged by size:
386 325 320 127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1116 1158
Size: 1116 42
HaeII r_GCGC'y
Cuts at: 0 731 1158
Size: 731 427
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 551 583 877 909 1158
Size: 496 32 23 32 294 32 249
Fragments arranged by size:
496 294 249 32 32 32 23
KasI G'GCGC_C
Cuts at: 0 727 1158
Size: 727 431
KpnI G_GTAC'C
Cuts at: 0 276 777 1158
Size: 276 501 381
Fragments arranged by size:
501 381 276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 494 1158
Size: 448 46 664
Fragments arranged by size:
664 448 46
MslI CAynn'nnrTG
Cuts at: 0 46 752 1158
Size: 46 706 406
Fragments arranged by size:
706 406 46
MspA1I CmG'CkG
Cuts at: 0 770 1158
Size: 770 388
NarI GG'CG_CC
Cuts at: 0 728 1158
Size: 728 430
NdeI CA'TA_TG
Cuts at: 0 117 1158
Size: 117 1041
NspI r_CATG'y
Cuts at: 0 661 1158
Size: 661 497
PasI CC'CwG_GG
Cuts at: 0 741 1158
Size: 741 417
PfoI T'CCnGG_A
Cuts at: 0 239 902 1158
Size: 239 663 256
Fragments arranged by size:
663 256 239
PpuMI rG'GwC_Cy
Cuts at: 0 386 706 1158
Size: 386 320 452
Fragments arranged by size:
452 386 320
PspOMI G'GGCC_C
Cuts at: 0 832 1158
Size: 832 326
PstI C_TGCA'G
Cuts at: 0 464 803 1158
Size: 464 339 355
Fragments arranged by size:
464 355 339
PvuII CAG'CTG
Cuts at: 0 770 1158
Size: 770 388
SacI G_AGCT'C
Cuts at: 0 74 1057 1158
Size: 74 983 101
Fragments arranged by size:
983 101 74
SanDI GG'GwC_CC
Cuts at: 0 706 1158
Size: 706 452
SfcI C'TryA_G
Cuts at: 0 460 799 1158
Size: 460 339 359
Fragments arranged by size:
460 359 339
SfoI GGC'GCC
Cuts at: 0 729 1158
Size: 729 429
SmaI CCC'GGG
Cuts at: 0 93 384 644 831 1158
Size: 93 291 260 187 327
Fragments arranged by size:
327 291 260 187 93
SmlI C'TyrA_G
Cuts at: 0 75 490 526 1158
Size: 75 415 36 632
Fragments arranged by size:
632 415 75 36
SspI AAT'ATT
Cuts at: 0 1120 1158
Size: 1120 38
StuI AGG'CCT
Cuts at: 0 496 1158
Size: 496 662
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 408 521 1158
Size: 408 113 637
Fragments arranged by size:
637 408 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 928 960 1158
Size: 928 32 198
Fragments arranged by size:
928 198 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 738 1158
Size: 15 723 420
Fragments arranged by size:
723 420 15
XmaI C'CCGG_G
Cuts at: 0 91 382 642 829 1158
Size: 91 291 260 187 329
Fragments arranged by size:
329 291 260 187 91
ZraI GAC'GTC
Cuts at: 0 888 1158
Size: 888 270
Enzymes that do cut:
AatII Acc65I AcuI AhdI AleI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BcgI BglI
BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BsrFI BstAPI BstBI BstXI BstYI BtgI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I
KasI KpnI MmeI MslI MspA1I NarI NdeI NspI
PasI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SfcI SfoI SmaI SmlI SspI StuI TaqII TstI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BciVI BclI BfrBI BlpI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaXI BsgI BsiWI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII EagI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MscI NaeI NcoI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI StyI SwaI TatI Tth111I XbaI XhoI
XmnI