Restriction map for
pF1KB6516
(Linear) (Six-base) MAPSORT of: KIBB6516.seq Check: 2913 from: 1 to: 1155
KIBB6516.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 583 1155
Size: 583 572
Acc65I G'GTAC_C
Cuts at: 0 655 1155
Size: 655 500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 672 678 696 743 858 1155
Size: 383 289 6 18 47 115 297
Fragments arranged by size:
383 297 289 115 47 18 6
ApaI G_GGCC'C
Cuts at: 0 605 1155
Size: 605 550
BanI G'GyrC_C
Cuts at: 0 655 1155
Size: 655 500
BanII G_rGCy'C
Cuts at: 0 395 605 1155
Size: 395 210 550
Fragments arranged by size:
550 395 210
BbsI GAAGACnn'nnnn_
Cuts at: 0 277 1155
Size: 277 878
BglII A'GATC_T
Cuts at: 0 428 1155
Size: 428 727
Bme1580I G_kGCm'C
Cuts at: 0 605 1155
Size: 605 550
BmgBI CAC'GTC
Cuts at: 0 335 1155
Size: 335 820
BmrI ACTGGGnnnn_n'
Cuts at: 0 387 600 749 1155
Size: 387 213 149 406
Fragments arranged by size:
406 387 213 149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 770 1155
Size: 286 484 385
Fragments arranged by size:
484 385 286
BsaBI GATnn'nnATC
Cuts at: 0 307 433 1155
Size: 307 126 722
Fragments arranged by size:
722 307 126
BsaHI Gr'CG_yC
Cuts at: 0 473 1155
Size: 473 682
BsaWI w'CCGG_w
Cuts at: 0 311 1155
Size: 311 844
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 536 566 1155
Size: 536 30 589
Fragments arranged by size:
589 536 30
BseYI C'CCAG_C
Cuts at: 0 651 1155
Size: 651 504
BsiHKAI G_wGCw'C
Cuts at: 0 67 1155
Size: 67 1088
BsmI GAATG_Cn'
Cuts at: 0 1069 1155
Size: 1069 86
Bsp1286I G_dGCh'C
Cuts at: 0 67 395 605 1155
Size: 67 328 210 550
Fragments arranged by size:
550 328 210 67
BspEI T'CCGG_A
Cuts at: 0 311 1155
Size: 311 844
BspHI T'CATG_A
Cuts at: 0 845 1155
Size: 845 310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 583 1155
Size: 583 572
BstYI r'GATC_y
Cuts at: 0 428 1155
Size: 428 727
BtgI C'CryG_G
Cuts at: 0 49 540 659 1155
Size: 49 491 119 496
Fragments arranged by size:
496 491 119 49
BtsI GCAGTG_nn'
Cuts at: 0 540 1155
Size: 540 615
DraIII CAC_nnn'GTG
Cuts at: 0 706 1155
Size: 706 449
EaeI y'GGCC_r
Cuts at: 0 537 866 1155
Size: 537 329 289
Fragments arranged by size:
537 329 289
EarI CTCTTCn'nnn_
Cuts at: 0 1045 1155
Size: 1045 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 265 1155
Size: 265 890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 383 672 678 696 743 770 858
Size: 286 97 289 6 18 47 27 88
Cuts at: 858 1155
Size: 297
Fragments arranged by size:
297 289 286 97 88 47 27 18
6
EcoO109I rG'GnC_Cy
Cuts at: 0 602 642 1155
Size: 602 40 513
Fragments arranged by size:
602 513 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 808 840 1155
Size: 808 32 315
Fragments arranged by size:
808 315 32
KpnI G_GTAC'C
Cuts at: 0 659 1155
Size: 659 496
MscI TGG'CCA
Cuts at: 0 539 868 1155
Size: 539 329 287
Fragments arranged by size:
539 329 287
MslI CAynn'nnrTG
Cuts at: 0 54 1048 1155
Size: 54 994 107
Fragments arranged by size:
994 107 54
MspA1I CmG'CkG
Cuts at: 0 716 1155
Size: 716 439
NcoI C'CATG_G
Cuts at: 0 49 540 659 1155
Size: 49 491 119 496
Fragments arranged by size:
496 491 119 49
NspI r_CATG'y
Cuts at: 0 893 1155
Size: 893 262
PflMI CCAn_nnn'nTGG
Cuts at: 0 185 864 1155
Size: 185 679 291
Fragments arranged by size:
679 291 185
PsiI TTA'TAA
Cuts at: 0 1031 1155
Size: 1031 124
PspOMI G'GGCC_C
Cuts at: 0 601 1155
Size: 601 554
PstI C_TGCA'G
Cuts at: 0 10 1155
Size: 10 1145
PvuII CAG'CTG
Cuts at: 0 716 1155
Size: 716 439
SfcI C'TryA_G
Cuts at: 0 6 852 1155
Size: 6 846 303
Fragments arranged by size:
846 303 6
StyI C'CwwG_G
Cuts at: 0 49 540 659 1155
Size: 49 491 119 496
Fragments arranged by size:
496 491 119 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 327 1155
Size: 82 245 828
Fragments arranged by size:
828 245 82
TatI w'GTAC_w
Cuts at: 0 667 1155
Size: 667 488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 773 1155
Size: 773 382
XmnI GAAnn'nnTTC
Cuts at: 0 88 1155
Size: 88 1067
Enzymes that do cut:
AarI Acc65I AcuI ApaI BanI BanII BbsI BglII
Bme1580I BmgBI BmrI BpmI BsaBI BsaHI BsaWI BsaXI
BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BstYI
BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I
Hin4I KpnI MscI MslI MspA1I NcoI NspI PflMI
PsiI PspOMI PstI PvuII SfcI StyI TaqII TatI
XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BseRI BsgI BsiEI BsiWI BsmBI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
XmaI ZraI