Restriction map for
pF1KB6514
(Linear) (Six-base) MAPSORT of: KIBB6514.seq Check: 3416 from: 1 to: 1113
KIBB6514.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 875 1113
Size: 875 238
Acc65I G'GTAC_C
Cuts at: 0 1052 1113
Size: 1052 61
AleI CACnn'nnGTG
Cuts at: 0 523 1113
Size: 523 590
AlwNI CAG_nnn'CTG
Cuts at: 0 104 675 856 1113
Size: 104 571 181 257
Fragments arranged by size:
571 257 181 104
ApoI r'AATT_y
Cuts at: 0 196 1113
Size: 196 917
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 468 501 1113
Size: 468 33 612
Fragments arranged by size:
612 468 33
BanI G'GyrC_C
Cuts at: 0 1052 1113
Size: 1052 61
BanII G_rGCy'C
Cuts at: 0 391 1113
Size: 391 722
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 972 1006 1113
Size: 972 34 107
Fragments arranged by size:
972 107 34
BclI T'GATC_A
Cuts at: 0 510 1113
Size: 510 603
BmrI ACTGGGnnnn_n'
Cuts at: 0 866 1113
Size: 866 247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 334 478 1113
Size: 72 262 144 635
Fragments arranged by size:
635 262 144 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 375 1113
Size: 375 738
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 207 237 332 362 1055 1085 1113
Size: 207 30 95 30 693 30 28
Fragments arranged by size:
693 207 95 30 30 30 28
BseYI C'CCAG_C
Cuts at: 0 607 652 683 1113
Size: 607 45 31 430
Fragments arranged by size:
607 430 45 31
BsiEI CG_ry'CG
Cuts at: 0 483 1113
Size: 483 630
BsiHKAI G_wGCw'C
Cuts at: 0 391 1113
Size: 391 722
Bsp1286I G_dGCh'C
Cuts at: 0 391 1113
Size: 391 722
BsrDI GCAATG_nn'
Cuts at: 0 84 1113
Size: 84 1029
BsrFI r'CCGG_y
Cuts at: 0 704 1113
Size: 704 409
BstXI CCAn_nnnn'nTGG
Cuts at: 0 537 1113
Size: 537 576
Bsu36I CC'TnA_GG
Cuts at: 0 44 1113
Size: 44 1069
BtgI C'CryG_G
Cuts at: 0 35 1028 1113
Size: 35 993 85
Fragments arranged by size:
993 85 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1020 1113
Size: 1020 93
BtsI GCAGTG_nn'
Cuts at: 0 944 1113
Size: 944 169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 806 841 1113
Size: 806 35 272
Fragments arranged by size:
806 272 35
DraI TTT'AAA
Cuts at: 0 335 1089 1113
Size: 335 754 24
Fragments arranged by size:
754 335 24
EaeI y'GGCC_r
Cuts at: 0 480 597 1025 1031 1113
Size: 480 117 428 6 82
Fragments arranged by size:
480 428 117 82 6
EagI C'GGCC_G
Cuts at: 0 480 1113
Size: 480 633
EarI CTCTTCn'nnn_
Cuts at: 0 214 1113
Size: 214 899
EcoICRI GAG'CTC
Cuts at: 0 389 1113
Size: 389 724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 334 478 1113
Size: 72 262 144 635
Fragments arranged by size:
635 262 144 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 399 1113
Size: 399 714
EcoO109I rG'GnC_Cy
Cuts at: 0 672 1113
Size: 672 441
EcoRI G'AATT_C
Cuts at: 0 196 1113
Size: 196 917
HaeII r_GCGC'y
Cuts at: 0 711 1113
Size: 711 402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 332 364 1113
Size: 332 32 749
Fragments arranged by size:
749 332 32
HincII GTy'rAC
Cuts at: 0 165 291 1113
Size: 165 126 822
Fragments arranged by size:
822 165 126
HpaI GTT'AAC
Cuts at: 0 291 1113
Size: 291 822
KpnI G_GTAC'C
Cuts at: 0 1056 1113
Size: 1056 57
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1113
Size: 85 1028
MscI TGG'CCA
Cuts at: 0 599 1033 1113
Size: 599 434 80
Fragments arranged by size:
599 434 80
MslI CAynn'nnrTG
Cuts at: 0 523 1113
Size: 523 590
MspA1I CmG'CkG
Cuts at: 0 611 1113
Size: 611 502
NcoI C'CATG_G
Cuts at: 0 35 1028 1113
Size: 35 993 85
Fragments arranged by size:
993 85 35
NdeI CA'TA_TG
Cuts at: 0 375 1113
Size: 375 738
SacI G_AGCT'C
Cuts at: 0 391 1113
Size: 391 722
StyI C'CwwG_G
Cuts at: 0 35 1028 1113
Size: 35 993 85
Fragments arranged by size:
993 85 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 848 1113
Size: 848 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 207 239 1055 1087 1113
Size: 207 32 816 32 26
Fragments arranged by size:
816 207 32 32 26
Enzymes that do cut:
AccI Acc65I AleI AlwNI ApoI BaeI BanI BanII
BcgI BclI BmrI BpmI BsaI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BsrDI BsrFI BstXI Bsu36I BtgI BtgZI
BtsI CspCI DraI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII HpaI KpnI
MmeI MscI MslI MspA1I NcoI NdeI SacI StyI
TaqII TstI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BbeI BbsI BbvCI BciVI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I ClaI DraIII
DrdI EciI EcoRV FalI FseI FspI FspAI HindIII
KasI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI