Restriction map for
pF1KB6503
(Linear) (Six-base) MAPSORT of: KIBB6503.seq Check: 2965 from: 1 to: 1074
KIBB6503.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
Acc65I G'GTAC_C
Cuts at: 0 665 1074
Size: 665 409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 1074
Size: 20 1054
AflIII A'CryG_T
Cuts at: 0 857 1074
Size: 857 217
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 844 876 1074
Size: 844 32 198
Fragments arranged by size:
844 198 32
AlwNI CAG_nnn'CTG
Cuts at: 0 425 496 756 1074
Size: 425 71 260 318
Fragments arranged by size:
425 318 260 71
ApaLI G'TGCA_C
Cuts at: 0 308 1074
Size: 308 766
BanI G'GyrC_C
Cuts at: 0 665 1074
Size: 665 409
BanII G_rGCy'C
Cuts at: 0 809 1074
Size: 809 265
BbsI GAAGACnn'nnnn_
Cuts at: 0 639 901 1074
Size: 639 262 173
Fragments arranged by size:
639 262 173
BbvCI CC'TCA_GC
Cuts at: 0 5 1074
Size: 5 1069
BciVI GTATCCnnnnn_n'
Cuts at: 0 883 1074
Size: 883 191
BfrBI ATG'CAT
Cuts at: 0 281 1074
Size: 281 793
Bme1580I G_kGCm'C
Cuts at: 0 305 312 1074
Size: 305 7 762
Fragments arranged by size:
762 305 7
BmgBI CAC'GTC
Cuts at: 0 1067 1074
Size: 1067 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 365 673 823 886 1024 1074
Size: 35 330 308 150 63 138 50
Fragments arranged by size:
330 308 150 138 63 50 35
Bpu10I CC'TnA_GC
Cuts at: 0 5 1074
Size: 5 1069
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 1074
Size: 691 383
BsaI GGTCTCn'nnnn_
Cuts at: 0 979 1074
Size: 979 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 1074
Size: 193 30 851
Fragments arranged by size:
851 193 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 368 1074
Size: 55 313 706
Fragments arranged by size:
706 313 55
BsiHKAI G_wGCw'C
Cuts at: 0 312 620 917 1074
Size: 312 308 297 157
Fragments arranged by size:
312 308 297 157
BsmI GAATG_Cn'
Cuts at: 0 283 1074
Size: 283 791
Bsp1286I G_dGCh'C
Cuts at: 0 305 312 620 809 917 1074
Size: 305 7 308 189 108 157
Fragments arranged by size:
308 305 189 157 108 7
BspHI T'CATG_A
Cuts at: 0 231 1074
Size: 231 843
BsrBI CCG'CTC
Cuts at: 0 15 1074
Size: 15 1059
BsrFI r'CCGG_y
Cuts at: 0 715 1074
Size: 715 359
BstYI r'GATC_y
Cuts at: 0 86 1074
Size: 86 988
Bsu36I CC'TnA_GG
Cuts at: 0 503 1074
Size: 503 571
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 287 1074
Size: 287 787
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 531 566 1074
Size: 531 35 508
Fragments arranged by size:
531 508 35
DraIII CAC_nnn'GTG
Cuts at: 0 819 1074
Size: 819 255
EaeI y'GGCC_r
Cuts at: 0 464 1074
Size: 464 610
EarI CTCTTCn'nnn_
Cuts at: 0 347 1074
Size: 347 727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 35 365 673 823 886 1024 1074
Size: 20 15 330 308 150 63 138 50
Fragments arranged by size:
330 308 150 138 63 50 20 15
EcoO109I rG'GnC_Cy
Cuts at: 0 45 422 1074
Size: 45 377 652
Fragments arranged by size:
652 377 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 210 1074
Size: 210 864
HaeII r_GCGC'y
Cuts at: 0 944 1074
Size: 944 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 1074
Size: 379 32 663
Fragments arranged by size:
663 379 32
KpnI G_GTAC'C
Cuts at: 0 669 1074
Size: 669 405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1074
Size: 944 130
MscI TGG'CCA
Cuts at: 0 466 1074
Size: 466 608
MslI CAynn'nnrTG
Cuts at: 0 982 1074
Size: 982 92
MspA1I CmG'CkG
Cuts at: 0 493 783 927 1074
Size: 493 290 144 147
Fragments arranged by size:
493 290 147 144
NaeI GCC'GGC
Cuts at: 0 717 1074
Size: 717 357
NgoMIV G'CCGG_C
Cuts at: 0 715 1074
Size: 715 359
NsiI A_TGCA'T
Cuts at: 0 283 1074
Size: 283 791
NspI r_CATG'y
Cuts at: 0 861 987 1074
Size: 861 126 87
Fragments arranged by size:
861 126 87
PciI A'CATG_T
Cuts at: 0 857 1074
Size: 857 217
PfoI T'CCnGG_A
Cuts at: 0 863 1074
Size: 863 211
PpuMI rG'GwC_Cy
Cuts at: 0 422 1074
Size: 422 652
PvuII CAG'CTG
Cuts at: 0 493 783 927 1074
Size: 493 290 144 147
Fragments arranged by size:
493 290 147 144
SapI GCTCTTCn'nnn_
Cuts at: 0 347 1074
Size: 347 727
SexAI A'CCwGG_T
Cuts at: 0 424 1074
Size: 424 650
SmlI C'TyrA_G
Cuts at: 0 670 1074
Size: 670 404
SpeI A'CTAG_T
Cuts at: 0 333 1074
Size: 333 741
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 809 1074
Size: 809 265
TatI w'GTAC_w
Cuts at: 0 200 1074
Size: 200 874
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 816 1074
Size: 816 258
Enzymes that do cut:
Acc65I AcuI AflIII AloI AlwNI ApaLI BanI BanII
BbsI BbvCI BciVI BfrBI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspHI
BsrBI BsrFI BstYI Bsu36I BtgZI CspCI DraIII EaeI
EarI Eco57MI EcoO109I FalI HaeII Hin4I KpnI MmeI
MscI MslI MspA1I NaeI NgoMIV NsiI NspI PciI
PfoI PpuMI PvuII SapI SexAI SmlI SpeI TaqII
TatI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BclI BglI BglII
BlpI BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgI BtsI ClaI DraI DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI NarI NcoI NdeI NheI
NotI NruI PacI PasI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI
StuI StyI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI