Restriction map for
pF1KB6490
(Linear) (Six-base) MAPSORT of: KIBB6490.seq Check: 7500 from: 1 to: 1071
KIBB6490.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 361 1071
Size: 361 710
AccI GT'mk_AC
Cuts at: 0 377 695 1071
Size: 377 318 376
Fragments arranged by size:
377 376 318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1071
Size: 142 929
AleI CACnn'nnGTG
Cuts at: 0 292 1071
Size: 292 779
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2 34 1071
Size: 2 32 1037
Fragments arranged by size:
1037 32 2
AlwNI CAG_nnn'CTG
Cuts at: 0 1044 1071
Size: 1044 27
BamHI G'GATC_C
Cuts at: 0 35 1071
Size: 35 1036
BanII G_rGCy'C
Cuts at: 0 346 1071
Size: 346 725
BbsI GAAGACnn'nnnn_
Cuts at: 0 954 1071
Size: 954 117
BbvCI CC'TCA_GC
Cuts at: 0 886 1071
Size: 886 185
BciVI GTATCCnnnnn_n'
Cuts at: 0 261 1071
Size: 261 810
BglI GCCn_nnn'nGGC
Cuts at: 0 350 1071
Size: 350 721
Bme1580I G_kGCm'C
Cuts at: 0 299 367 799 1001 1071
Size: 299 68 432 202 70
Fragments arranged by size:
432 299 202 70 68
BmgBI CAC'GTC
Cuts at: 0 921 1071
Size: 921 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 697 1071
Size: 697 374
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 226 1071
Size: 226 845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 569 733 813 1071
Size: 40 529 164 80 258
Fragments arranged by size:
529 258 164 80 40
Bpu10I CC'TnA_GC
Cuts at: 0 886 1071
Size: 886 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 664 769 1071
Size: 601 63 105 302
Fragments arranged by size:
601 302 105 63
BsaAI yAC'GTr
Cuts at: 0 384 1071
Size: 384 687
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 38 58 68 88 1071
Size: 38 20 10 20 983
Fragments arranged by size:
983 38 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 772 1071
Size: 772 299
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 901 1071
Size: 505 396 170
Fragments arranged by size:
505 396 170
BsmI GAATG_Cn'
Cuts at: 0 126 1071
Size: 126 945
Bsp1286I G_dGCh'C
Cuts at: 0 299 346 367 799 1001 1071
Size: 299 47 21 432 202 70
Fragments arranged by size:
432 299 202 70 47 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 361 1071
Size: 361 710
BsrDI GCAATG_nn'
Cuts at: 0 563 1071
Size: 563 508
BsrGI T'GTAC_A
Cuts at: 0 836 1071
Size: 836 235
BstXI CCAn_nnnn'nTGG
Cuts at: 0 31 257 1071
Size: 31 226 814
Fragments arranged by size:
814 226 31
BstYI r'GATC_y
Cuts at: 0 35 1071
Size: 35 1036
BstZ17I GTA'TAC
Cuts at: 0 378 1071
Size: 378 693
Bsu36I CC'TnA_GG
Cuts at: 0 115 1071
Size: 115 956
BtgI C'CryG_G
Cuts at: 0 359 1071
Size: 359 712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 753 788 1071
Size: 753 35 283
Fragments arranged by size:
753 283 35
EaeI y'GGCC_r
Cuts at: 0 351 1071
Size: 351 720
EarI CTCTTCn'nnn_
Cuts at: 0 142 1071
Size: 142 929
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 871 1071
Size: 871 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 142 569 733 813 1071
Size: 40 102 427 164 80 258
Fragments arranged by size:
427 258 164 102 80 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1036 1071
Size: 1036 35
EcoO109I rG'GnC_Cy
Cuts at: 0 1041 1071
Size: 1041 30
HincII GTy'rAC
Cuts at: 0 44 1071
Size: 44 1027
MscI TGG'CCA
Cuts at: 0 353 1071
Size: 353 718
MslI CAynn'nnrTG
Cuts at: 0 94 255 292 1071
Size: 94 161 37 779
Fragments arranged by size:
779 161 94 37
MspA1I CmG'CkG
Cuts at: 0 489 1071
Size: 489 582
NcoI C'CATG_G
Cuts at: 0 359 1071
Size: 359 712
NspI r_CATG'y
Cuts at: 0 837 1071
Size: 837 234
PflMI CCAn_nnn'nTGG
Cuts at: 0 360 1044 1071
Size: 360 684 27
Fragments arranged by size:
684 360 27
PpuMI rG'GwC_Cy
Cuts at: 0 1041 1071
Size: 1041 30
PvuII CAG'CTG
Cuts at: 0 489 1071
Size: 489 582
SfcI C'TryA_G
Cuts at: 0 279 435 1071
Size: 279 156 636
Fragments arranged by size:
636 279 156
SmlI C'TyrA_G
Cuts at: 0 616 679 784 1071
Size: 616 63 105 287
Fragments arranged by size:
616 287 105 63
SspI AAT'ATT
Cuts at: 0 625 1071
Size: 625 446
StyI C'CwwG_G
Cuts at: 0 359 1071
Size: 359 712
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 679 1071
Size: 679 392
TatI w'GTAC_w
Cuts at: 0 836 1071
Size: 836 235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 38 70 1071
Size: 38 32 1001
Fragments arranged by size:
1001 38 32
Tth111I GACn'n_nGTC
Cuts at: 0 245 1071
Size: 245 826
XmnI GAAnn'nnTTC
Cuts at: 0 124 1071
Size: 124 947
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI BamHI BanII
BbsI BbvCI BciVI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BsgI BsmI
Bsp1286I BspMI BsrDI BsrGI BstXI BstYI BstZ17I Bsu36I
BtgI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I
HincII MscI MslI MspA1I NcoI NspI PflMI PpuMI
PvuII SfcI SmlI SspI StyI TaqII TatI TstI
Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BcgI BclI BfrBI BglII
BlpI BmtI BsaI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BtgZI BtsI ClaI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI XbaI XcmI XhoI
XmaI ZraI