Restriction map for
pF1KB6489
(Linear) (Six-base) MAPSORT of: KIBB6489.seq Check: 8029 from: 1 to: 987
KIBB6489.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 28 595 987
Size: 28 567 392
Fragments arranged by size:
567 392 28
Acc65I G'GTAC_C
Cuts at: 0 12 987
Size: 12 975
AclI AA'CG_TT
Cuts at: 0 978 987
Size: 978 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 561 987
Size: 358 203 426
Fragments arranged by size:
426 358 203
AflIII A'CryG_T
Cuts at: 0 366 403 987
Size: 366 37 584
Fragments arranged by size:
584 366 37
AlwNI CAG_nnn'CTG
Cuts at: 0 25 775 865 987
Size: 25 750 90 122
Fragments arranged by size:
750 122 90 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 168 201 987
Size: 168 33 786
Fragments arranged by size:
786 168 33
BanI G'GyrC_C
Cuts at: 0 12 987
Size: 12 975
BbsI GAAGACnn'nnnn_
Cuts at: 0 479 987
Size: 479 508
BciVI GTATCCnnnnn_n'
Cuts at: 0 169 241 987
Size: 169 72 746
Fragments arranged by size:
746 169 72
BglII A'GATC_T
Cuts at: 0 964 987
Size: 964 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 191 987
Size: 191 796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 987
Size: 646 341
BsaI GGTCTCn'nnnn_
Cuts at: 0 564 987
Size: 564 423
BsaWI w'CCGG_w
Cuts at: 0 242 827 987
Size: 242 585 160
Fragments arranged by size:
585 242 160
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 619 649 987
Size: 619 30 338
Fragments arranged by size:
619 338 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 77 987
Size: 77 910
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 705 987
Size: 652 53 282
Fragments arranged by size:
652 282 53
BsmI GAATG_Cn'
Cuts at: 0 501 987
Size: 501 486
BspEI T'CCGG_A
Cuts at: 0 827 987
Size: 827 160
BspHI T'CATG_A
Cuts at: 0 557 987
Size: 557 430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 28 595 987
Size: 28 567 392
Fragments arranged by size:
567 392 28
BsrDI GCAATG_nn'
Cuts at: 0 860 987
Size: 860 127
BsrGI T'GTAC_A
Cuts at: 0 695 987
Size: 695 292
BstXI CCAn_nnnn'nTGG
Cuts at: 0 223 293 987
Size: 223 70 694
Fragments arranged by size:
694 223 70
BstYI r'GATC_y
Cuts at: 0 69 964 987
Size: 69 895 23
Fragments arranged by size:
895 69 23
BtsI GCAGTG_nn'
Cuts at: 0 640 987
Size: 640 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 561 646 987
Size: 358 203 85 341
Fragments arranged by size:
358 341 203 85
EcoO109I rG'GnC_Cy
Cuts at: 0 118 515 987
Size: 118 397 472
Fragments arranged by size:
472 397 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 775 790 807 822 987
Size: 775 15 17 15 165
Fragments arranged by size:
775 165 17 15 15
KpnI G_GTAC'C
Cuts at: 0 16 987
Size: 16 971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 833 987
Size: 335 498 154
Fragments arranged by size:
498 335 154
MslI CAynn'nnrTG
Cuts at: 0 562 987
Size: 562 425
MspA1I CmG'CkG
Cuts at: 0 133 987
Size: 133 854
NspI r_CATG'y
Cuts at: 0 370 407 987
Size: 370 37 580
Fragments arranged by size:
580 370 37
PciI A'CATG_T
Cuts at: 0 366 403 987
Size: 366 37 584
Fragments arranged by size:
584 366 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 148 226 987
Size: 148 78 761
Fragments arranged by size:
761 148 78
PpuMI rG'GwC_Cy
Cuts at: 0 118 987
Size: 118 869
PstI C_TGCA'G
Cuts at: 0 39 987
Size: 39 948
PvuII CAG'CTG
Cuts at: 0 133 987
Size: 133 854
SbfI CC_TGCA'GG
Cuts at: 0 39 987
Size: 39 948
SfcI C'TryA_G
Cuts at: 0 35 180 987
Size: 35 145 807
Fragments arranged by size:
807 145 35
StuI AGG'CCT
Cuts at: 0 78 987
Size: 78 909
StyI C'CwwG_G
Cuts at: 0 73 987
Size: 73 914
TatI w'GTAC_w
Cuts at: 0 695 987
Size: 695 292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 619 651 987
Size: 619 32 336
Fragments arranged by size:
619 336 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 223 621 987
Size: 223 398 366
Fragments arranged by size:
398 366 223
Enzymes that do cut:
AarI Acc65I AclI AcuI AflIII AlwNI BaeI BanI
BbsI BciVI BglII BmrI BpmI BsaI BsaWI BsaXI
BseRI BsgI BsmI BspEI BspHI BspMI BsrDI BsrGI
BstXI BstYI BtsI Eco57MI EcoO109I Hin4I KpnI MmeI
MslI MspA1I NspI PciI PflMI PpuMI PstI PvuII
SbfI SfcI StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AatII AccI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BamHI BanII BbeI BbvCI BcgI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaHI BseYI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XhoI XmaI XmnI ZraI