Restriction map for
pF1KB6477
(Linear) (Six-base) MAPSORT of: KIBB6477.seq Check: 3274 from: 1 to: 978
KIBB6477.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 781 978
Size: 781 197
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 978
Size: 394 584
AhdI GACnn_n'nnGTC
Cuts at: 0 118 978
Size: 118 860
AlwNI CAG_nnn'CTG
Cuts at: 0 75 372 558 978
Size: 75 297 186 420
Fragments arranged by size:
420 297 186 75
ApoI r'AATT_y
Cuts at: 0 226 238 455 978
Size: 226 12 217 523
Fragments arranged by size:
523 226 217 12
BamHI G'GATC_C
Cuts at: 0 504 978
Size: 504 474
BanI G'GyrC_C
Cuts at: 0 131 725 978
Size: 131 594 253
Fragments arranged by size:
594 253 131
BanII G_rGCy'C
Cuts at: 0 143 403 978
Size: 143 260 575
Fragments arranged by size:
575 260 143
BbeI G_GCGC'C
Cuts at: 0 729 978
Size: 729 249
BglI GCCn_nnn'nGGC
Cuts at: 0 283 978
Size: 283 695
BlpI GC'TnA_GC
Cuts at: 0 46 936 978
Size: 46 890 42
Fragments arranged by size:
890 46 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 71 978
Size: 71 907
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 763 978
Size: 46 717 215
Fragments arranged by size:
717 215 46
BsaHI Gr'CG_yC
Cuts at: 0 726 978
Size: 726 252
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 481 488 978
Size: 478 3 7 490
Fragments arranged by size:
490 478 7 3
BseYI C'CCAG_C
Cuts at: 0 955 978
Size: 955 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 978
Size: 88 890
BsiHKAI G_wGCw'C
Cuts at: 0 143 978
Size: 143 835
BsmBI CGTCTCn'nnnn_
Cuts at: 0 126 978
Size: 126 852
Bsp1286I G_dGCh'C
Cuts at: 0 143 403 978
Size: 143 260 575
Fragments arranged by size:
575 260 143
BssSI C'ACGA_G
Cuts at: 0 851 978
Size: 851 127
BstAPI GCAn_nnn'nTGC
Cuts at: 0 66 978
Size: 66 912
BstYI r'GATC_y
Cuts at: 0 504 555 978
Size: 504 51 423
Fragments arranged by size:
504 423 51
BstZ17I GTA'TAC
Cuts at: 0 782 978
Size: 782 196
Bsu36I CC'TnA_GG
Cuts at: 0 500 978
Size: 500 478
BtsI GCAGTG_nn'
Cuts at: 0 910 978
Size: 910 68
EarI CTCTTCn'nnn_
Cuts at: 0 416 978
Size: 416 562
EcoICRI GAG'CTC
Cuts at: 0 141 978
Size: 141 837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 394 763 978
Size: 46 348 369 215
Fragments arranged by size:
369 348 215 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 512 978
Size: 512 466
EcoO109I rG'GnC_Cy
Cuts at: 0 112 220 517 960 978
Size: 112 108 297 443 18
Fragments arranged by size:
443 297 112 108 18
EcoRI G'AATT_C
Cuts at: 0 226 978
Size: 226 752
HaeII r_GCGC'y
Cuts at: 0 25 729 978
Size: 25 704 249
Fragments arranged by size:
704 249 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 104 136 415 447 790 822 978
Size: 104 32 279 32 343 32 156
Fragments arranged by size:
343 279 156 104 32 32 32
HindIII A'AGCT_T
Cuts at: 0 166 545 844 978
Size: 166 379 299 134
Fragments arranged by size:
379 299 166 134
KasI G'GCGC_C
Cuts at: 0 725 978
Size: 725 253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 788 978
Size: 615 173 190
Fragments arranged by size:
615 190 173
MspA1I CmG'CkG
Cuts at: 0 372 667 759 978
Size: 372 295 92 219
Fragments arranged by size:
372 295 219 92
NarI GG'CG_CC
Cuts at: 0 726 978
Size: 726 252
NspI r_CATG'y
Cuts at: 0 701 978
Size: 701 277
PpuMI rG'GwC_Cy
Cuts at: 0 112 220 517 960 978
Size: 112 108 297 443 18
Fragments arranged by size:
443 297 112 108 18
PstI C_TGCA'G
Cuts at: 0 516 978
Size: 516 462
PvuII CAG'CTG
Cuts at: 0 372 667 759 978
Size: 372 295 92 219
Fragments arranged by size:
372 295 219 92
SacI G_AGCT'C
Cuts at: 0 143 978
Size: 143 835
SanDI GG'GwC_CC
Cuts at: 0 112 978
Size: 112 866
SfcI C'TryA_G
Cuts at: 0 512 978
Size: 512 466
SfoI GGC'GCC
Cuts at: 0 727 978
Size: 727 251
Tth111I GACn'n_nGTC
Cuts at: 0 75 978
Size: 75 903
Enzymes that do cut:
AccI AcuI AhdI AlwNI ApoI BamHI BanI BanII
BbeI BglI BlpI BplI BpmI BsaHI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BssSI BstAPI BstYI BstZ17I
Bsu36I BtsI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I HindIII KasI MmeI MspA1I NarI NspI
PpuMI PstI PvuII SacI SanDI SfcI SfoI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstBI BstEII BstXI BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoRV FalI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI MscI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI XbaI XcmI
XhoI XmaI XmnI ZraI