Restriction map for
pF1KB6475
(Linear) (Six-base) MAPSORT of: KIBB6475.seq Check: 7798 from: 1 to: 978
KIBB6475.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 947 978
Size: 947 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 978
Size: 853 125
AflIII A'CryG_T
Cuts at: 0 297 978
Size: 297 681
AleI CACnn'nnGTG
Cuts at: 0 595 978
Size: 595 383
AlwNI CAG_nnn'CTG
Cuts at: 0 343 906 978
Size: 343 563 72
Fragments arranged by size:
563 343 72
ApaLI G'TGCA_C
Cuts at: 0 246 978
Size: 246 732
BanI G'GyrC_C
Cuts at: 0 64 197 978
Size: 64 133 781
Fragments arranged by size:
781 133 64
BbeI G_GCGC'C
Cuts at: 0 68 978
Size: 68 910
BbsI GAAGACnn'nnnn_
Cuts at: 0 728 978
Size: 728 250
BbvCI CC'TCA_GC
Cuts at: 0 193 978
Size: 193 785
BclI T'GATC_A
Cuts at: 0 675 978
Size: 675 303
Bme1580I G_kGCm'C
Cuts at: 0 250 353 882 978
Size: 250 103 529 96
Fragments arranged by size:
529 250 103 96
BmgBI CAC'GTC
Cuts at: 0 699 978
Size: 699 279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 365 559 627 978
Size: 223 142 194 68 351
Fragments arranged by size:
351 223 194 142 68
Bpu10I CC'TnA_GC
Cuts at: 0 193 978
Size: 193 785
BsaI GGTCTCn'nnnn_
Cuts at: 0 199 978
Size: 199 779
BsaBI GATnn'nnATC
Cuts at: 0 674 978
Size: 674 304
BsaHI Gr'CG_yC
Cuts at: 0 65 978
Size: 65 913
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 978
Size: 166 812
BseYI C'CCAG_C
Cuts at: 0 852 946 978
Size: 852 94 32
Fragments arranged by size:
852 94 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 978
Size: 160 818
BsiHKAI G_wGCw'C
Cuts at: 0 218 250 561 978
Size: 218 32 311 417
Fragments arranged by size:
417 311 218 32
BsmI GAATG_Cn'
Cuts at: 0 46 978
Size: 46 932
Bsp1286I G_dGCh'C
Cuts at: 0 218 250 353 561 882 978
Size: 218 32 103 208 321 96
Fragments arranged by size:
321 218 208 103 96 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 947 978
Size: 947 31
BsrBI CCG'CTC
Cuts at: 0 165 978
Size: 165 813
BsrFI r'CCGG_y
Cuts at: 0 145 978
Size: 145 833
BstEII G'GTnAC_C
Cuts at: 0 456 978
Size: 456 522
BstXI CCAn_nnnn'nTGG
Cuts at: 0 324 597 978
Size: 324 273 381
Fragments arranged by size:
381 324 273
BstYI r'GATC_y
Cuts at: 0 600 978
Size: 600 378
BtgI C'CryG_G
Cuts at: 0 596 978
Size: 596 382
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 412 978
Size: 412 566
ClaI AT'CG_AT
Cuts at: 0 806 978
Size: 806 172
DraIII CAC_nnn'GTG
Cuts at: 0 548 978
Size: 548 430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 978
Size: 64 914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 365 559 627 853 978
Size: 223 142 194 68 226 125
Fragments arranged by size:
226 223 194 142 125 68
EcoO109I rG'GnC_Cy
Cuts at: 0 903 978
Size: 903 75
EcoRV GAT'ATC
Cuts at: 0 804 978
Size: 804 174
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 844 978
Size: 844 134
HaeII r_GCGC'y
Cuts at: 0 68 978
Size: 68 910
KasI G'GCGC_C
Cuts at: 0 64 978
Size: 64 914
MfeI C'AATT_G
Cuts at: 0 782 978
Size: 782 196
MslI CAynn'nnrTG
Cuts at: 0 454 595 978
Size: 454 141 383
Fragments arranged by size:
454 383 141
MspA1I CmG'CkG
Cuts at: 0 467 978
Size: 467 511
NarI GG'CG_CC
Cuts at: 0 65 978
Size: 65 913
NruI TCG'CGA
Cuts at: 0 431 978
Size: 431 547
NspI r_CATG'y
Cuts at: 0 162 301 978
Size: 162 139 677
Fragments arranged by size:
677 162 139
PciI A'CATG_T
Cuts at: 0 297 978
Size: 297 681
PflMI CCAn_nnn'nTGG
Cuts at: 0 343 906 978
Size: 343 563 72
Fragments arranged by size:
563 343 72
PfoI T'CCnGG_A
Cuts at: 0 899 978
Size: 899 79
PpuMI rG'GwC_Cy
Cuts at: 0 903 978
Size: 903 75
SfcI C'TryA_G
Cuts at: 0 507 873 978
Size: 507 366 105
Fragments arranged by size:
507 366 105
SfoI GGC'GCC
Cuts at: 0 66 978
Size: 66 912
SphI G_CATG'C
Cuts at: 0 162 978
Size: 162 816
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 905 978
Size: 905 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1 33 978
Size: 1 32 945
Fragments arranged by size:
945 32 1
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 978
Size: 180 798
XmnI GAAnn'nnTTC
Cuts at: 0 79 635 978
Size: 79 556 343
Fragments arranged by size:
556 343 79
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaLI BanI BbeI
BbsI BbvCI BclI Bme1580I BmgBI BpmI Bpu10I BsaI
BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BstEII BstXI BstYI BtgI BtgZI
ClaI DraIII EciI Eco57MI EcoO109I EcoRV FalI HaeII
KasI MfeI MslI MspA1I NarI NruI NspI PciI
PflMI PfoI PpuMI SfcI SfoI SphI TaqII TstI
XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BcgI BciVI BfrBI BglI
BglII BlpI BmrI BmtI BplI BpuEI BsaAI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtsI CspCI
DraI DrdI EaeI EagI EarI EcoICRI EcoNI EcoRI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NsiI PacI PasI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI TatI Tth111I XbaI XhoI
XmaI ZraI