(Linear) (Six-base) MAPSORT of: KIBB6465.seq Check: 3174 from: 1 to: 966 KIBB6465.orf With 182 enzymes: SgfI * April 24, 2009 13:05 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 332 966 Size: 332 634 AhdI GACnn_n'nnGTC Cuts at: 0 504 966 Size: 504 462 AseI AT'TA_AT Cuts at: 0 197 743 842 966 Size: 197 546 99 124 Fragments arranged by size: 546 197 124 99 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 113 146 966 Size: 113 33 820 Fragments arranged by size: 820 113 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 500 914 966 Size: 500 414 52 Fragments arranged by size: 500 414 52 BclI T'GATC_A Cuts at: 0 213 966 Size: 213 753 BglII A'GATC_T Cuts at: 0 151 966 Size: 151 815 BmrI ACTGGGnnnn_n' Cuts at: 0 134 966 Size: 134 832 BsaBI GATnn'nnATC Cuts at: 0 218 966 Size: 218 748 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 670 966 Size: 670 296 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 454 917 966 Size: 454 463 49 Fragments arranged by size: 463 454 49 BstAPI GCAn_nnn'nTGC Cuts at: 0 814 966 Size: 814 152 BstYI r'GATC_y Cuts at: 0 151 405 966 Size: 151 254 561 Fragments arranged by size: 561 254 151 Bsu36I CC'TnA_GG Cuts at: 0 605 966 Size: 605 361 BtgI C'CryG_G Cuts at: 0 631 966 Size: 631 335 ClaI AT'CG_AT Cuts at: 0 730 966 Size: 730 236 DraI TTT'AAA Cuts at: 0 431 966 Size: 431 535 EarI CTCTTCn'nnn_ Cuts at: 0 892 966 Size: 892 74 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 332 966 Size: 332 634 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 132 966 Size: 132 834 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 490 522 966 Size: 490 32 444 Fragments arranged by size: 490 444 32 HindIII A'AGCT_T Cuts at: 0 109 966 Size: 109 857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 97 966 Size: 97 869 NcoI C'CATG_G Cuts at: 0 631 966 Size: 631 335 StyI C'CwwG_G Cuts at: 0 178 631 966 Size: 178 453 335 Fragments arranged by size: 453 335 178 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 648 966 Size: 648 318 Enzymes that do cut: AcuI AhdI AseI BaeI BbsI BclI BglII BmrI BsaBI BseRI BsgI BstAPI BstYI Bsu36I BtgI ClaI DraI EarI Eco57MI FalI Hin4I HindIII MmeI NcoI StyI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI BtsI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI