(Linear) (Six-base) MAPSORT of: KIBB6465.seq  Check: 3174  from: 1  to: 966



KIBB6465.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     966 
   Size:        332     634
 
AhdI GACnn_n'nnGTC

Cuts at:      0     504     966 
   Size:        504     462
 
AseI AT'TA_AT

Cuts at:      0     197     743     842     966 
   Size:        197     546      99     124

  Fragments arranged by size: 

                 546     197     124      99
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     113     146     966 
   Size:        113      33     820

  Fragments arranged by size: 

                 820     113      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     500     914     966 
   Size:        500     414      52

  Fragments arranged by size: 

                 500     414      52
 
BclI T'GATC_A

Cuts at:      0     213     966 
   Size:        213     753
 
BglII A'GATC_T

Cuts at:      0     151     966 
   Size:        151     815
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     134     966 
   Size:        134     832
 
BsaBI GATnn'nnATC

Cuts at:      0     218     966 
   Size:        218     748
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     670     966 
   Size:        670     296
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454     917     966 
   Size:        454     463      49

  Fragments arranged by size: 

                 463     454      49
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     814     966 
   Size:        814     152
 
BstYI r'GATC_y

Cuts at:      0     151     405     966 
   Size:        151     254     561

  Fragments arranged by size: 

                 561     254     151
 
Bsu36I CC'TnA_GG

Cuts at:      0     605     966 
   Size:        605     361
 
BtgI C'CryG_G

Cuts at:      0     631     966 
   Size:        631     335
 
ClaI AT'CG_AT

Cuts at:      0     730     966 
   Size:        730     236
 
DraI TTT'AAA

Cuts at:      0     431     966 
   Size:        431     535
 
EarI CTCTTCn'nnn_

Cuts at:      0     892     966 
   Size:        892      74
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     966 
   Size:        332     634
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     132     966 
   Size:        132     834
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     490     522     966 
   Size:        490      32     444

  Fragments arranged by size: 

                 490     444      32
 
HindIII A'AGCT_T

Cuts at:      0     109     966 
   Size:        109     857
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     966 
   Size:         97     869
 
NcoI C'CATG_G

Cuts at:      0     631     966 
   Size:        631     335
 
StyI C'CwwG_G

Cuts at:      0     178     631     966 
   Size:        178     453     335

  Fragments arranged by size: 

                 453     335     178
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     648     966 
   Size:        648     318
 

 Enzymes that do cut:

     AcuI     AhdI     AseI     BaeI     BbsI     BclI    BglII     BmrI
    BsaBI    BseRI     BsgI   BstAPI    BstYI   Bsu36I     BtgI     ClaI
     DraI     EarI  Eco57MI     FalI    Hin4I  HindIII     MmeI     NcoI
     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI