(Linear) (Six-base) MAPSORT of: KIBB6460.seq  Check: 760  from: 1  to: 1053



KIBB6460.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     768    1053 
   Size:        523     245     285

  Fragments arranged by size: 

                 523     285     245
 
AfeI AGC'GCT

Cuts at:      0     841    1053 
   Size:        841     212
 
ApaI G_GGCC'C

Cuts at:      0     672    1053 
   Size:        672     381
 
ApoI r'AATT_y

Cuts at:      0      52    1053 
   Size:         52    1001
 
AvaI C'yCGr_G

Cuts at:      0     319    1053 
   Size:        319     734
 
BanI G'GyrC_C

Cuts at:      0     601    1053 
   Size:        601     452
 
BanII G_rGCy'C

Cuts at:      0     160     425     672    1053 
   Size:        160     265     247     381

  Fragments arranged by size: 

                 381     265     247     160
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     963    1053 
   Size:        963      90
 
BglII A'GATC_T

Cuts at:      0     737     828    1053 
   Size:        737      91     225

  Fragments arranged by size: 

                 737     225      91
 
Bme1580I G_kGCm'C

Cuts at:      0     374     672    1053 
   Size:        374     298     381

  Fragments arranged by size: 

                 381     374     298
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     405    1053 
   Size:        405     648
 
Bpu10I CC'TnA_GC

Cuts at:      0     634    1053 
   Size:        634     419
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838    1053 
   Size:        838     215
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     187    1017    1053 
   Size:        187     830      36

  Fragments arranged by size: 

                 830     187      36
 
BsaAI yAC'GTr

Cuts at:      0     369    1053 
   Size:        369     684
 
BsaBI GATnn'nnATC

Cuts at:      0     913    1053 
   Size:        913     140
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     457    1053 
   Size:        457     596
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     759    1053 
   Size:        759     294
 
Bsp1286I G_dGCh'C

Cuts at:      0     160     374     425     672    1053 
   Size:        160     214      51     247     381

  Fragments arranged by size: 

                 381     247     214     160      51
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     812     813    1053 
   Size:        812       1     240

  Fragments arranged by size: 

                 812     240       1
 
BsrBI CCG'CTC

Cuts at:      0     103    1053 
   Size:        103     950
 
BstYI r'GATC_y

Cuts at:      0     218     483     737     828    1053 
   Size:        218     265     254      91     225

  Fragments arranged by size: 

                 265     254     225     218      91
 
BtgI C'CryG_G

Cuts at:      0     656    1053 
   Size:        656     397
 
DraI TTT'AAA

Cuts at:      0     957    1053 
   Size:        957      96
 
EaeI y'GGCC_r

Cuts at:      0     695    1053 
   Size:        695     358
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     417    1053 
   Size:        417     636
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     768    1053 
   Size:        523     245     285

  Fragments arranged by size: 

                 523     285     245
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     820    1053 
   Size:        820     233
 
EcoO109I rG'GnC_Cy

Cuts at:      0     498     669     801     961    1053 
   Size:        498     171     132     160      92

  Fragments arranged by size: 

                 498     171     160     132      92
 
EcoRI G'AATT_C

Cuts at:      0      52    1053 
   Size:         52    1001
 
EcoRV GAT'ATC

Cuts at:      0     873    1053 
   Size:        873     180
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      97     786    1053 
   Size:         97     689     267

  Fragments arranged by size: 

                 689     267      97
 
HaeII r_GCGC'y

Cuts at:      0     843    1053 
   Size:        843     210
 
HincII GTy'rAC

Cuts at:      0     297     867    1053 
   Size:        297     570     186

  Fragments arranged by size: 

                 570     297     186
 
MslI CAynn'nnrTG

Cuts at:      0     358     544    1053 
   Size:        358     186     509

  Fragments arranged by size: 

                 509     358     186
 
MspA1I CmG'CkG

Cuts at:      0     700    1053 
   Size:        700     353
 
PasI CC'CwG_GG

Cuts at:      0     672     964    1053 
   Size:        672     292      89

  Fragments arranged by size: 

                 672     292      89
 
PpuMI rG'GwC_Cy

Cuts at:      0     498     801    1053 
   Size:        498     303     252

  Fragments arranged by size: 

                 498     303     252
 
PspOMI G'GGCC_C

Cuts at:      0     668    1053 
   Size:        668     385
 
PspXI vC'TCGA_Gb

Cuts at:      0     319    1053 
   Size:        319     734
 
PstI C_TGCA'G

Cuts at:      0     824    1053 
   Size:        824     229
 
SbfI CC_TGCA'GG

Cuts at:      0     824    1053 
   Size:        824     229
 
SfcI C'TryA_G

Cuts at:      0     492     820    1053 
   Size:        492     328     233

  Fragments arranged by size: 

                 492     328     233
 
SmlI C'TyrA_G

Cuts at:      0     319     853    1053 
   Size:        319     534     200

  Fragments arranged by size: 

                 534     319     200
 
XhoI C'TCGA_G

Cuts at:      0     319    1053 
   Size:        319     734
 
XmnI GAAnn'nnTTC

Cuts at:      0      52      77    1053 
   Size:         52      25     976

  Fragments arranged by size: 

                 976      52      25
 

 Enzymes that do cut:

     AcuI     AfeI     ApaI     ApoI     AvaI     BanI    BanII    BciVI
    BglII Bme1580I     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
     BsgI    BsmBI Bsp1286I    BspMI    BsrBI    BstYI     BtgI     DraI
     EaeI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
    HaeII   HincII     MslI   MspA1I     PasI    PpuMI   PspOMI    PspXI
     PstI     SbfI     SfcI     SmlI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BpmI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EarI  EcoICRI     FseI
     FspI    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI